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Sypro orange protein gel stain 5 000x concentrate in dmso

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SYPRO® Orange Protein Gel Stain (5,000X Concentrate in DMSO) is a fluorescent dye that can be used to stain proteins in polyacrylamide gels. It is a 5,000X concentrated solution in dimethyl sulfoxide (DMSO).

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Hispur cobalt resin, by Thermo Fisher Scientific (1 mentions) Amicon ultra 15 centrifugal filter unit, by Merck Group (1 mentions) Lb broth, by Thermo Fisher Scientific (1 mentions) Envision 2104 multilabel reader, by PerkinElmer (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Biowest (1 mentions)

Spelling variants of this product

SYPRO Orange (691 citations) SYPRO Orange Protein Gel Stain (82 citations) SYPRO Orange protein stain (10 citations) SYPRO Orange stain (3 citations) Sypro Orange (5×) (3 citations)

3 protocols using sypro orange protein gel stain 5 000x concentrate in dmso

1

Synthesis and Characterization of Gold Nanoparticles

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Gold (III) chloride trihydrate (HAuCl4) (PubChem ID: 24895143; Product number: G4022), sodium citrate dihydrate (C6H5Na3O7) (PubChem ID: 24901436; Product number: W302600), L-ascorbic acid (L-AA) (PubChem ID: 24891246; Product number: A7506), silver nitrate (AgNO3) (PubChem ID: 24852543; Product number: S0139), hyaluronic acid (HA) sodium salt from Streptococcus equi (MW: 7,000–250,000 g.mol-1) (PubChem ID: 24878223; Product number: 53747), oleic acid (OA) (MW: 282.46 g.mol-1) (PubChem ID: 24886786; Product number: 75090), Lysozyme from hen egg white, in powder form, (LYZ, MW: 14.3 kDa) (Enzyme number:3.2.1.17; Product number: L6876) were all supplied by Sigma-Aldrich (Steinheim, Germany). SYPRO® Orange Protein Gel Stain (5,000X Concentrate in DMSO) was purchased from Life Technologies as the molecular probe for protein conformational studies. The water used for buffer preparation was purified through a Millipore system.
Oliveira Silva C., Petersen S.B., Pinto Reis C., Rijo P., Molpeceres J., Vorum H, & Neves-Petersen M.T. (2015). Lysozyme Photochemistry as a Function of Temperature. The Protective Effect of Nanoparticles on Lysozyme Photostability. PLoS ONE, 10(12), e0144454.
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2

Protein Purification and Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reagents were obtained from the following manufacturers: LB Broth, Ampicillin
Sodium Salt, HisPur Cobalt Resin (Fisher Scientific, Waltham, MA); IPTG (RPI
Corp., Mt. Prospect, IL); Complete EDTA-Free Protease Inhibitor Cocktail
Tablets, Lightcycler 480 II RT-PCR (Roche Applied Science, Indianapolis, IN);
SYPRO® Orange Protein Gel Stain 5000x Concentrate in DMSO (Life
Technologies, Grand Island, NY); 2-Mercaptoethanol, Electrophoresis ≥98%,
(Fisher BioReagents, Waltham, MA); DMSO (Sigma Aldrich, St. Louis, MO); Amicon
Ultra-15 Centrifugal Filter Units (EMD Millipore, Billerica, MA); MultiTron
Incubated Shaker (INFORS HT, Bottmingen, Switzerland); Ultrasonic Liquid
Processor (Misonix, Inc., Farmingdale, NY); ÄKTA purifier FPLC, Nanodrop
Spectrophotometer ND-1000 (GE Healthcare Life Sciences, Piscataway, NJ);
Envision 2104 multilabel reader (Perkin Elmer, Waltham, MA); Prestwick Chemical
Library® (Prestwick Chemical, Illkirch, France); NIH
Clinical Collection 1 and 2 (Evotec, San Francisco, CA); Custom Clinical
Collection (provided by Cliff Stephan at the GCC); National Cancer Institute
Diversity, Natural Products, Mechanistic, and Challenge Sets (NCI/NIH
Developmental Therapeutics Program, Bethesda, MD); BL21 Star™ (DE3),
Competent E. coli, PolarScreen™ ER-β Competitor
Assay, Green and PolarScreen™ ER-α Competitor Assay, Green (Life
Technologies, Carlsbad, CA).
Filgueira C.S., Benod C., Lou X., Gunamalai P.S., Villagomez R.A., Strom A., Gustafsson J.Å., Berkenstam A.L, & Webb P. (2014). A screening cascade to identify ERβ ligands. Nuclear Receptor Signaling, 12, e003.
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3

Gold Nanoparticle Synthesis and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gold (III) chloride trihydrate (HAuCl4) (PubChem ID: 24895143; Product number: G4022), L-ascorbic acid (L-AA) (PubChem ID: 24891246; Product number: A7506), silver nitrate (AgNO3) (PubChem ID: 24852543; Product number: S0139), hyaluronic acid (HA) sodium salt from Streptococcus equi (MW: 7,000–250,000 g.mol-1) (PubChem ID: 24878223; Product number: 53747), oleic acid (OA) (MW: 282.46 g.mol-1) (PubChem ID: 24886786; Product number: 75090) were all supplied by Sigma-Aldrich (Steinheim, Germany). Recombinant Human Epidermal Growth Factor (EGF) (PubChem ID: 62253638), Alexa Fluor® 647 and SYPRO® Orange Protein Gel Stain (5,000X Concentrate in DMSO) was purchased from Life Technologies as molecular probes for confocal microscopy and protein conformational studies. Primary mouse monoclonal antibody anti-EGFR neutralizer antibody LA1 was obtained from Millipore (05–101). The water used for buffer preparation was purified through a Millipore system. Thiazolyl Blue Tetrazolium Bromide (MTT), Fetal Bovine Serum (FBS), puromycin and penicillin/streptomycin were supplied by Sigma-Aldrich (Steinheim, Germany), as of cell culture grade. Dulbecco's Modified Eagle's medium (DMEM) was supplied by Biowest (Nuaillé, France) and DMSO was supplied by Merck (Darmstadt, Germany).
Silva C.O., Petersen S.B., Reis C.P., Rijo P., Molpeceres J., Fernandes A.S., Gonçalves O., Gomes A.C., Correia I., Vorum H, & Neves-Petersen M.T. (2016). EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy. PLoS ONE, 11(10), e0165419.
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