Gotaq green master

Manufactured by Promega

Sourced in United States

GoTaq® Green Master is a ready-to-use solution for PCR amplification. It contains all the necessary components, including GoTaq® DNA Polymerase, dNTPs, MgCl2, and a green dye, for direct loading onto an agarose gel after the PCR reaction.

Automatically generated - may contain errors

Lab products found in correlation

Revertaid rt reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Zymo quick rna miniprep kit, by Zymo Research (1 mentions) Lysostaphin, by Merck Group (1 mentions) Mastercycler gradient, by Eppendorf (1 mentions)

4 protocols using gotaq green master

RNA Extraction, Reverse Transcription, and PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA extraction, reverse transcription, and PCR or quantitative PCR (qPCR) were performed as previously described [29 ]. In brief, total RNA was extracted from mouse tissues by using TRIzol reagent (Life Technologies, Carlsbad, CA). Total RNA was pre-treated with an DNase Ι and 5 μg of treated RNA was used as template for first-strand complementary DNA (cDNA) synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies, Carlsbad, CA). Aliquots of the RT products (50 ng) were used for regular and quantitative RT-PCR. Quantitative RT-PCR (qPCR) was performed using Radiant™ SYBR Green Hi-ROX qPCR Kits (Alkali Scientific, Pompano Beach, FL) in StepOnePlus™ Real-Time PCR Systems (Life Technologies, Carlsbad, CA) and normalized to glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Regular RT-PCR was performed using GoTaq® Green Master (Promega, Madison, WI). The primers used in this study are listed in Additional file 1: Table S1.

Xu J., El Refaey M., Xu L., Zhao L., Gao Y., Floyd K., Karaze T., Janssen P.M, & Han R. (2015). Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy. Skeletal Muscle, 5, 43.

+ Open protocol

+ Expand

Genomic DNA and RNA Extraction for qPCR Analysis

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA was extracted from mouse tissues, C2C12, HEK293 and RAW 264.7 cells. Total RNA was extracted from C2C12 and HEK293 cells by using Zymo Quick-RNA™ MiniPrep kit (Zymo Research Corporation, CA, USA). Real-time PCR were performed as previously described (30 (link),34 (link),35 (link)). Briefly, 20 ng genomic DNA was used as template and RT-PCR products were analyzed for gene editing efficiency. Real-time PCR reactions were performed using Radiant™ SYBR Green Hi-ROX qPCR Kits (Alkali Scientific, Pompano Beach, FL) in LightCycler^® 480 System and normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Regular RT-PCR was performed using GoTaq^® Green Master (Promega, Madison, WI, USA).

Xu L., Zhao L., Gao Y., Xu J, & Han R. (2016). Empower multiplex cell and tissue-specific CRISPR-mediated gene manipulation with self-cleaving ribozymes and tRNA. Nucleic Acids Research, 45(5), e28.

+ Open protocol

+ Expand

Cloning and Characterizing OSC Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

PmLSS of Patiria miniata was cloned from embryo (2d old) cDNA. PpOSC1 and PpOSC2 were cloned from mixed cDNA of P. parvimensis early developmental stages (embryo 2d, larvae 4d). These were PCR-amplified using primers with attB1 and attB2 adapters and cloned into pDONOR207 (Gen^R, Invitrogen) through a BP recombination reaction. PCR conditions were as follows: initial denaturation (95°C, 1 min); 30 cycles of 95°C for 30 s; 58°C for 1 min; 72°C for 4 min; final elongation step of 7 min at 72°C. The PCR reaction mix contained: 2μl cDNA; 10 μM of each primer; 250 μM of each dNTP; 1x Phusion buffer; and 1U of Phusion DNA polymerase (NEB). After BP reaction and transformation colony PCR was performed using GoTaq^® Green Master (Promega) and positive clones were validated by sequencing. Positive clones were sequenced using three sets of primers covering entire length of the gene (~2.2 Kb). The bacterial OSC candidate was cloned from genomic DNA of Gemmata obscuriglobus DSM5831^T strain (GoOSC). Error free clones were then cloned into pYES2 (Amp^R) through yeast homologous recombination. All primers used are listed in Supplementary Table 9 (from Sigma-Aldrich).

Thimmappa R., Wang S., Zheng M., Misra R.C., Huang A.C., Saalbach G., Chang Y., Zhou Z., Hinman V., Bao Z, & Osbourn A. (2022). Biosynthesis of saponin defensive compounds in sea cucumbers. Nature Chemical Biology, 18(7), 774-781.

+ Open protocol

+ Expand

Detection of ESBL-Producing Bacterial Isolates

Check if the same lab product or an alternative is used in the 5 most similar protocols

From the sum total of isolates analysed (11 phenotypically confirmed ESBL producers only 6 randomly selected and were subjected to polymerase chain reaction (PCR) analysis. The PCR amplification of bla genes, including blaCTX-M bla AmpC were carried out with GoTaq® Green Master Mix using primers listed in (Table 1). DNA extraction: A single colony from overnight incubated pure culture was suspended into 1ml of distilled water, centrifuged at 14000xg for 2 min., then the supernatant discarded, after that 120μL of lysostaphin (10 mg/L; Sigma) was added. DNA extracted using mini DNA extraction kit (Promega) according to manufacture instructions. PCR were carried out using thermal cycler (Eppendorf Master cycler Gradient) depending on [14 & 15) . The PCR mix was prepared in a volume of 25 μl containing 4 μl DNA template, 12.5 μl Go Taq Green Master (Promega, CA) 2

Hamasaeed P.A., & Hyder S.J. (2020). Silver nanoparticle Bacteriostatic activity Assessment against Extended-Spectrum Beta-Lactamase Producing Uropathogenic Klebsiella pneumonia. Journal of Raparin University, 7(2), 288-304.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!