Accu check 2 glucometer

To assess the effects of the WD and psychological stress on cardiometabolic risk factors, non‐fasted blood was collected at sacrifice to assess circulating levels glucose, triglycerides, insulin, and corticosterone. Glucose was determined immediately in whole blood using an Accu‐check II glucometer (Roche Diagnostic). Serum samples were stored at −80°C for subsequent metabolic analyses. Serum triglycerides were assayed using a triglyceride quantification colorimetric kit (Biovision Inc.), serum insulin using an ultra‐sensitive mouse insulin ELISA kit (Crystal Chem), and corticosterone using a Corticosterone ELISA kit (Enzo LifeScience), according to manufacturer's instructions. The balance between circulating insulin and glucose levels was determined using the homeostasis model assessment of insulin resistance (HOMA‐IR), however as non‐fasted blood samples were utilized, it can only be used to infer insulin resistance. $$\begin{array}{c}\text{HOMA}\text{IR}=\frac{[\text{mg}{\text{dl}}^{‐1}\text{glucose}\times \mathrm{\mu }\mathrm{U}{\text{ml}}^{‐1}\text{insulin}]}{405}\hfill \end{array}$$

Mice were fasted for 6 hours and then injected intraperitoneally with glucose (2 g/kg); blood glucose levels were monitored over time using an Accu-check II glucometer (Roche Diagnostics, Castle Hill, NSW, Australia). Fasting blood insulins as well as insulin levels during the GTT were measured using an Ultrasensitive Mouse Insulin Elisa Kit (Crystal Chem, Illinois, USA). For in vivo insulin stimulations, mice were fasted overnight and given an intraperitoneal injection of insulin at 2U/kg lean mass, with tissues rapidly excised and snap frozen 15 min after the injection.

Serum samples were obtained by the immediate centrifugation of blood collected from the orbital sinus into heparin or EDTA. Plasma for biochemical or hormonal analyses was stored at −70 °C until use. Insulin and glucagon were determined with the MO DIABETES INSULIN SET and MO DIABETES GLUCAGON SET (BIO-RAD), respectively, by immunoassay on a Luminex apparatus (Biorad). The kits for determining alanine aminotransferase (ALAT) and triglyceride (TG) levels were purchased from DiaSys. Capillary blood glucose level was determined with an Accu-Check II Glucometer (Roche Diagnostic).