The largest database of trusted experimental protocols

102 protocols using q150r es

1

Hydrogel Patch Characterization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tensile strength and percentage elongation break testThe hydrogel patch was cut in a rectangle shape (1 × 4 cm2) and evaluated by a tensile tester (TA.XT plus, Stable Micro Systems, UK). The cross-section of the hydrogel patch was fixed by adhesive tape between the cell grips. The test conditions were the test speed of 1 mm/s and the trigger force of 5 g. Force was gradually applied until the hydrogel patch broke.
ThicknessThe thickness at five different positions on the GM hydrogel patch was measured using an analog thickness gauge.
Physical characteristicsThe color of the GM hydrogel patch was measured using Chroma Meter CR-400 (Konica Minolta, Japan). The CIE L* a* b* (L, lightness; a, redness; and b, yellowness) were determined.
Surface morphologyThe surface morphology of the GM hydrogel patch and blank-hydrogel patch were studied. All samples were incubated at 40 °C for 72 h to remove the moisture before testing. The dried samples were coated with gold (QUORUM Q150R ES, UK) and evaluated with a field emission scanning electron microscope (FE-SEM; JEOL JSM7800F, Japan) and imaged at an acceleration voltage of 5 kV.
+ Open protocol
+ Expand
2

SEM Analysis of Material Topography

Check if the same lab product or an alternative is used in the 5 most similar protocols
Scanning electron microscope (SEM) (FEI, ISPECT S50) was used to examine the specimens’ surface characteristics (topography). To avoid the non-conductive property of the material, a gold coating was applied with a sputter coating machine (Quorum, Q150R ES, UK). Images were captured at different magnifications (500, 1000, 2000, 5000, and 10000×) to observe important characteristics of the surface changes with different ND concentrations.
+ Open protocol
+ Expand
3

Fiber Morphology and Composition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibre morphology was characterised using SEM and IMAGE J software (National Institutes of Health, Bethesda, MD, USA). The fibres were sputter-coated with gold (Q150R ES, Quorum Technologies, Lewes, UK) for 180 s prior to being imaged by SEM (JEOL JSM-6301F or FEI Inspect-F SEM). Average fibre diameter was calculated by measuring the width of 100 fibres inn IMAGE J.
Chemical composition of the fibres was assessed using energy-dispersive X-ray spectroscopy (EDX) and performed using INCA X-Sight (Oxford Instruments, Abingdon, UK). The voltage used was 20 kV and the working distance was 10 mm. INCA software (ETAS, Derby, UK) was used to analyse the EDX spectra.
+ Open protocol
+ Expand
4

Scanning Electron Microscopy of AXGL5 Films

Check if the same lab product or an alternative is used in the 5 most similar protocols
The surface morphologies of the AXGL5 and AXGL5D films were studied using a scanning electron microscope (SEM) (FEI, Inspect S50, Brno, Czech Republic). For this purpose, AX-GL film samples were fixed on the SEM metallic stub using double-sided adhesive tape. These samples were then coated with gold (Quorum, Q150R ES, Lewes, UK). The SEM was operated at an accelerating voltage of 20 KV, and micrographs were captured at different magnifications.
+ Open protocol
+ Expand
5

Electrospun Membrane Characterization by SEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
The morphology of the electrospun membranes was studied by scanning electron microscopy (SEM) in a JEOL JSM-7500F equipment. The dried samples were metallized in a Quorum Q150R ES by depositing a thin layer of gold/palladium (60/40 at%) on the membranes for 40 s for electrical conduction. The SEM images obtained at 10,000X and their further processing for obtaining the fiber and pore diameters were done by the free-software ImageJ by taking 50 fibers.
+ Open protocol
+ Expand
6

Morphological Analysis of Zein Nanofibers

Check if the same lab product or an alternative is used in the 5 most similar protocols
We have analyzed the surface morphology of zein and zein/Nano-CUR NFs through the employment of Field Emission-Scanning Electron Microscopy (FESEM) (MIRA3, TESCAN Co., Czech). The images have been taken at the accelerating voltage of 20 kV subsequent to performing the gold coating process (Q150R ES, Quorum Technologies, UK). We have measured the diameters of NF by the usage of Image-J software (https://imagej.net/Fiji) along with the sample size of 100 NF. In addition, the fibers that had contained fluorescent properties have been identified under the Olympus fluorescent microscope (IX53, Japan), which had been equipped with a digital camera (Olympus DP73, Japan).
+ Open protocol
+ Expand
7

Nanomaterial Characterization by SEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Scanning Electron Microscopy (SEM) was performed with an EVO25 microscope (Zeiss, Cambridge, UK). Before the analysis, the samples were coated with a 4-nm layer of 50 k gold alloy in a Quorum Q150R ES (Quorum, Laughton, UK). A total of 165 nanoparticles were measured in different areas of each sample. Then a non-linear regression of Gaussian type was performed to obtain the average size of the nanoparticles.
+ Open protocol
+ Expand
8

SEM Analysis of PLGA and CS-PLGA Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols
The surface morphology and actual sizes of uncoated PLGA and CS-PLGA particles were examined using Scanning Electron Microscopy (SEM) (JEOL JSM-7100F, Tokyo, Japan). Fine particles of each sample were mounted on EM metal stubs previously covered with double-sided carbon tapes and coated with a thin gold layer using sputter coater (Q150R ES, Quorum, Madrid, Spain). The particles were photographed at an acceleration voltage of 5 KV.
+ Open protocol
+ Expand
9

SEM Analysis of PMMA Dental Discs

Check if the same lab product or an alternative is used in the 5 most similar protocols
One randomly selected disc per group was used for the scanning electron microscope (SEM) procedure. The PMMA discs were initially fixed at room temperature in 2.5% glutaraldehyde solution. The discs were then dehydrated in ascending ethanol concentrations. Afterwards, the specimens were gold sputter-coated after mounting on metallic stubs (Quorum, Q150R ES, Lewes, UK). Samples were inspected under SEM instrument (Emcrafts, Gyeonggi-do, Korea) at 10kV at ×2000 magnification.
+ Open protocol
+ Expand
10

Characterization of Porous Monoliths

Check if the same lab product or an alternative is used in the 5 most similar protocols
The PCL/CS porous monoliths were then cut into slices and gold-coated (Q 150R ES, Quorum Technologies Ltd., Laughton, UK) to a depth of 15 nm before characterisation by scanning electron microscopy (SEM) (EVO LS15, Carl Zeiss AG, Jena, Germany) to determine their surface morphology.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!