The following laboratory workup was performed on all participants: (a) Complete blood picture using fully automated hematology analyzer (HORIBA ABX Micros 60, France) with blood film examination; (b) Serum calcium, phosphorus, alkaline phosphatase, liver enzymes using commercially available kits (Cobas 6000 analyzer, USA); (c) Serum ferritin using electrochemiluminescence technology on immunoassay analyzer Cobas 411 (Roche Diagnostics, Japan); (d) Parathyroid hormone assay immunoassay analyzer Cobas 411 (Roche Diagnostics, Japan).
Cobas 411
Manufactured by Roche
Sourced in Japan, Germany
The Cobas 411 is a compact, fully automated benchtop analyzer designed for routine clinical chemistry and immunochemistry testing. It offers a wide range of analytical capabilities, including the measurement of various analytes, enzymes, and proteins. The Cobas 411 is intended to provide reliable and efficient laboratory results to support medical decision-making.
Automatically generated - may contain errors
8 protocols using cobas 411
1
Comprehensive Metabolic Workup Protocol
2
Blood Glucose, Insulin, and C-Peptide Assay
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Blood glucose and insulin were determined at baseline and at all times mentioned above, and c-peptide was measured at baseline and every thirty minutes up to three hours. All parameters were assayed by commercial kit Roche Diagnostics Italy with Cobas Integra 400 Plus and Cobas 411 (Roche diagnostic, Monza, Italy).
3
Blood Biomarkers and Cardiac Imaging
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Blood samples were collected at admission for NT-proBNP and other routine blood tests. NT-proBNP measurements were made with a COBAS 411 Roche device. Transthoracic echocardiography was performed for all patients within 24 h after hospitalization. Coronary angiography was performed with a General Electric Optima device.
4
Troponin Measurement in Myocardial Injury
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Blood samples were drawn from a peripheral vein and hsTnT was measured using the new quantitative electrochemiluminescence immunoassay (Cobas 411, Roche Diagnostics, Mannheim, Germany), as described previously [17 (link)]. A concentration of 14 ng/L has been identified as the 99th percentile of a healthy reference population with a coefficient of variability of <10% [18 (link)]. Chronic myocardial injury was defined as increased but stable troponin levels in terms of a rise/fall ≤20% in serial measurements, and acute myocardial injury was defined as the rise or fall of troponin >20% in serial measurements [19 (link),20 (link)].
5
Glucose, Insulin, and C-Peptide Dynamics
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We measured circulating levels of glucose, insulin, and C-peptide at baseline and every 10 min in the first half hour, and every 30 min thereafter. All parameters were assayed by a commercial kit (Roche Diagnostics) with Cobas Integra 400 Plus and Cobas 411 (Roche Diagnostic).
6
Metabolic Biomarkers Assessment
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Fasting blood samples were collected between 9 and 11 a.m. Plasma glucose, cholesterol, HDL cholesterol, and triglycerides were analysed using Vitro 4000 (Ortho Clinical Diagnostics). The concentration of LDL cholesterol was calculated. Insulin levels were measured using the Elecsys Insulin kit in combination with the Cobas 411 (Roche Diagnostics, Germany) equipment. The homeostasis model assessment-estimated insulin resistance (HOMA-IR) index was calculated according to the original formula [32 (link)].
7
Perioperative Blood Tests for Anemia and Iron Deficiency
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JID: JINJ [m5G; April 29, 2020; 22:40 ] Perioperative laboratory tests were performed in blood samples drawn preoperatively (upon hospital admission) and at postoperative days (POD) 7 and 30, and included: haemoglobin (DXH-800, Beckman Coulter, Brea, CA, USA), iron status and creatinine (AU-5800, Beckman Coulter), troponin I (Unicel DXI 800, Beckman Coulter), NT-proBNP (Cobas 411, Roche Diagnostic, Manheim, Germany). Additionally, Hb concentration was assessed at POD 1 and at any time during hospitalisation, should it be deemed necessary by the attending physician. Anaemia was defined by haemoglobin < 13 g/dL [28] (link)[29] (link)[30] (link) , irrespective of gender, and ID by TSAT < 20% and/or ferritin < 100 ng/mL [29] (link) .
8
Serum Vitamin D and Mineral Levels
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Serum calcium, phosphorus, parathormone were determined by standard methods. Vitamin D level was measured as serum 25(OH)-Vitamin D with COBAS 411 (Roche Diagnostics) using electrochemiluminescent immunoassay (ECLIA) method [8] . Vitamin D levels were stratified as a deficiency (<20 ng/L), insufficiency (21-29 ng/L), and adequate (!30 ng/L) [8] .
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