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Ascend 500 mhz spectrometer

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The Ascend 500 MHz spectrometer is a nuclear magnetic resonance (NMR) spectrometer designed for high-resolution analysis of chemical samples. It operates at a frequency of 500 MHz and provides accurate and reliable data for researchers and scientists.

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Lab products found in correlation

Cary 100 spectrophotometer, by Agilent Technologies (1 mentions) 500 ms it, by Agilent Technologies (1 mentions) Model 920 liquid chromatograph, by Agilent Technologies (1 mentions) Avance 3 400 mhz ft nmr spectrometer, by Bruker (1 mentions) Silica gel 60, by Merck Group (1 mentions) Gc17agc, by Shimadzu (1 mentions) Gcms qp5050a mass spectrometer, by Shimadzu (1 mentions)

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500 MHz spectrometer (210 citations) Bruker spectrometers (71 citations) Ascend 500 (28 citations) Ascend 500 spectrometer (20 citations) Ascend 500 MHz NMR spectrometer (4 citations) Avance III HD Ascend 500 MHz spectrometer (4 citations) 500 Spectrometers (2 citations)

10 protocols using ascend 500 mhz spectrometer

1

Characterization of BSA-Antibody-AuNPs Conjugate

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The BSA, AFB1 antibody, and conjugated AuNPs were placed in a dialysis tube and then clipped at both ends. The samples were dialysed against 0.1 M sodium bicarbonate NaHCO3 for 12 h. A few drops of the conjugated AuNPs were added using a disposable pipette and dissolved in 1 mL of deuterated Dimethyl Sulfoxide (DMSO) and then transferred to an NMR tube for analysis. The samples were transferred to an NMR tube for analysis while using a Bruker Ascend 500 MHz spectrometer and deuterated DMSO-d6.
Sojinrin T., Liu K., Wang K., Cui D., J. Byrne H., Curtin J.F, & Tian F. (2019). Developing Gold Nanoparticles-Conjugated Aflatoxin B1 Antifungal Strips. International Journal of Molecular Sciences, 20(24), 6260.
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2

NMR Characterization of Compounds

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1H and 13C NMR spectra were collected on a Bruker Ascend 500 MHz spectrometer for the samples in CDCl3 or D2O with a TMS internal standard at 25 °C.
Heczko D., Hachuła B., Maksym P., Kamiński K., Zięba A., Orszulak L., Paluch M, & Kamińska E. (2022). The Effect of Various Poly (N-vinylpyrrolidone) (PVP) Polymers on the Crystallization of Flutamide. Pharmaceuticals, 15(8), 971.
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3

Stability of Benzotriazole Oxide (BO)

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The half-life of BO in 0.1 M phosphate buffer (pH 7.4) at 37 °C was measured on a Cary 100 spectrophotometer (Agilent Technologies) based on the absorbance at 315 nm (Supplemental Data S2). Additionally, BO stability was investigated by 1H NMR on an Ascend 500 MHz spectrometer (Bruker Corporation) in various organic solvents over time under ambient light. BO was stored neat (20 °C, < 1 day), in MeOH (< 1 day, 20 °C), in Et2O (4 °C, > 60 days), in CDCl3 (−20 °C, > 75 days), or in dioxane (4 to 20 °C, > 90 days, as 1% or 10% BO solutions).
Zarth A.T., Murphy S.E, & Hecht S.S. (2015). Benzene Oxide is a Substrate for Glutathione S-Transferases. Chemico-biological interactions, 242, 390-395.
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4

Synthetic Characterization of Novel Compounds

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All of the reagents and solvents were purchased from ACROS Organics, Asta-Tech, Maybridge, Santa Cruz Biotechnology and Sigma-Aldrich. The melting points were measured in an OptiMelt—Automated Melting Point System (MPA 100, Stanford Research Systems). The 1H and 13C NMR spectra were recorded on a Bruker Ascend 500 MHz spectrometer at frequencies of 500 MHz and 126 MHz and a Bruker Avance III 400 MHz FT-NMR spectrometer at frequencies of 400 MHz and 101 MHz using CDCl3-d6 or DMSO-d6 as the solvent and TMS as the internal standard. The NMR solvents were purchased from ACROS Organics. The chemical shifts (δ) are given in ppm and the values of the coupling constants (J) are reported in hertz (Hz). The purity of all of the compounds was tested using the HPLC/MS method. The HPLC-MS analyses were performed on a Varian model 920 liquid chromatograph equipped with a Varian 900-LC model autosampler, a gradient pump, a Varian Pro Star 510 model column oven and a Varian 380-LC model evaporative light scattering detection (ELSD) detector. This was coupled with a Varian 500-MS IT. The HRMS were determined using a Waters LCT Premier XE high resolution mass spectrometer with electrospray ionization (ESI).
Bak A., Kozik V., Kozakiewicz D., Gajcy K., Strub D.J., Swietlicka A., Stepankova S., Imramovsky A., Polanski J., Smolinski A, & Jampilek J. (2019). Novel Benzene-Based Carbamates for AChE/BChE Inhibition: Synthesis and Ligand/Structure-Oriented SAR Study. International Journal of Molecular Sciences, 20(7), 1524.
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5

Kinetics of hOGG1-Mediated Repair of oxoG

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A total of 40 μM of the protein, WT hOGG1 or hOGG1(D268N) mutant, was mixed with 0.5 mM oxoG-containing DNA duplex in a buffer of 50 mM Tris(-D11)–HCl (pH 7.5), 100 mM NaCl and 1 mM dithiothreitol. The DNA solution in the absence of protein contained 0.5 mM oxoG-containing DNA duplex in a buffer of 50 mM Tris(-D11)–HCl (pH 7.5), 100 mM NaCl and 1 mM dithiothreitol. The 1H NMR monitoring of catalytic reaction was carried out with Bruker Ascend 500 MHz spectrometer at 293 K. For each spectrum, 1024 scans were accumulated, resulting in experimental time of 1 h. The measurement was repeated 20 times to obtain the time course of hOGG1 catalysis.
Šebera J., Hattori Y., Sato D., Řeha D., Nencka R., Kohno T., Kojima C., Tanaka Y, & Sychrovský V. (2017). The mechanism of the glycosylase reaction with hOGG1 base-excision repair enzyme: concerted effect of Lys249 and Asp268 during excision of 8-oxoguanine. Nucleic Acids Research, 45(9), 5231-5242.
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6

Characterization of Organic Compounds

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Melting points were determined with a Büchi apparatus and are uncorrected. 1H NMR spectra were obtained with a Bruker Ascend 500 MHz spectrometer; δ values are in ppm, J values are in Hz. All exchangeable protons were confirmed by the addition of D2O. Mass spectra were recorded on an HP 1100-MSD series instrument. Thin-layer chromatography (TLC) was carried out on pre-coated TLC plates with silica gel 60 F254 (Fluka). For column chromatography, silica gel 60 (Merck) was used. Elemental analyses were determined on Fisons Instruments Model EA 1108 CHNS-O model analyzer and are within 0.4% of theoretical values. Purity of the compounds is ≥98%, according to elemental analysis data.
Spinaci A., Buccioni M., Dal Ben D., Maggi F., Marucci G., Francucci B., Santoni G., Lambertucci C, & Volpini R. (2022). A3 Adenosine Receptor Antagonists with Nucleoside Structures and Their Anticancer Activity. Pharmaceuticals, 15(2), 164.
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7

Synthesis and Characterization of Dibenzocyclooctadiyne

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Anhydrous and anaerobic solvents were obtained from purification columns (Pure Process Technology, Nashua, NH). All reagents were obtained from commercial sources and were used as received unless mentioned otherwise. All reactions were run under a nitrogen atmosphere and monitored by TLC using silica gel 60 F254 precoated plates (Silicycle). Column chromatography was performed on a CombiFlash® Rf 200 system using RediSep™ normal phase silica columns (ISCO, Inc., Lincoln, NE). Microwave experiments were conducted in a CEM Discover model 909150 using 10 mL microwave tubes (CEM Corporation, Matthews, NC). 1H NMR (500 MHz) and 13C NMR (125 Mhz) spectra were recorded on a Bruker Ascend™ 500 Mhz spectrometer at room temperature and peaks were calibrated against an internal TMS standard. High-resolution mass spectra were recorded on a Thermo Fisher Scientific LTQ Orbitrap Discovery™. 3-Dimensional images were generated using Crystalmaker®: a crystal and molecular structures program for Mac and Windows. Crystalmaker Software Ltd, Oxford, England (www.crystalmaker.com). POAV angles were measured using mol2mol (www.gunda.hu/mol2mol/index.html). Dibenzocyclooctadiyne (3) was synthesized according to a literature procedure.[14 (link)]
Miller R.W., Duncan A.K., Schneebeli S.T., Gray D.L, & Whalley A.C. (2014). Synthesis and Structural Data of Tetrabenzo[8]circulene. Chemistry (Weinheim an der Bergstrasse, Germany), 20(13), 3705-3711.
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8

NMR Spectroscopic Analysis of SMP-3a

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About 50 mg of SMP-3a was dissolved in 0.5 mL of D2O in an NMR tube, and then the 1H NMR, 13C NMR, NOESY, COSY, HSQC, and HMBC spectra were recorded on a Bruker Ascend 500 MHz spectrometer at room temperature (25 °C).
Yue H., Xu Q., Li X., Elango J., Wu W, & Xu J. (2019). Physicochemical Characterization and Immunomodulatory Activity of a Novel Acid Polysaccharide from Solanum muricatum. Polymers, 11(12), 1972.
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9

NMR Spectroscopic Characterization of Polymers

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The 1H and 13C NMR spectra of the intermediates were recorded on a Bruker Ascend 500 MHz spectrometer by using deuterochloroform (CDCl3) as the solvent at room temperature. The 1H spectra of the polymers were recorded on a Bruker Ascend 500 MHz spectrometer by using dideutero-1,1,2,2-tetrachloroethane (C2D2Cl4) at 100 °C, which was also used to estimate the molecular weight. The purity of the polymers was verified by elemental analysis carried out at Midwest Microlabs Inc.
Huang W., Chen J., Yao Y., Zheng D., Ji X., Feng L.W., Moore D., Glavin N.R., Xie M., Chen Y., Pankow R.M., Surendran A., Wang Z., Xia Y., Bai L., Rivnay J., Ping J., Guo X., Cheng Y., Marks T.J, & Facchetti A. (2023). Vertical organic electrochemical transistors for complementary circuits. Nature, 613(7944), 496-502.
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10

Analysis of Tcmucins and GIPL Structures

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All solvents were purchased from Tedia (Fairfield, OH, USA). Resins and columns were acquired from BioRad (Richmond, CA, USA), and Restek (Bellefonte, PA, USA). Other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gas chromatography coupled with mass spectrometry (GC-MS) experiments were run in a Shimadzu GC-17A GC coupled with a Shimadzu GCMS-QP5050A mass spectrometer.
NMR experiments for the analysis of Tcmucins and GIPL structures were carried out on a Bruker Ascend 500 MHz spectrometer equipped with a 5 mm BBI gradient probe at the Centro Nacional de Ressonância Magnética Nuclear, UFRJ, Brazil.
Fonseca L.M., Garcez T.C., Penha L., Freire-DE-Lima L., Maes E., Costa K.M., Mendonça-Previato L, & Previato J.O. (2016). Expanding the knowledge of the chemical structure of glycoconjugates from Trypanosoma cruzi TcI genotype. Contribution to taxonomic studies. Anais da Academia Brasileira de Ciencias, 88(3).
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