Anti ctbp2

Immunoblotting analyses were carried out as described previously^{24 (link)}. Digital images were captured using the LAS-1000 Plus (Fujifilm). The following primary antibodies were used: mouse anti-Flag M2 (Sigma), anti-Myc 9E10 (Santa Cruz), and anti-β-actin 2F3 (Wako); rabbit anti-Myc and anti-SP-B (Santa Cruz), anti-CtBP1, anti-CtBP2, anti-SP-C, anti-Muc1 and anti-Abca3 (Abcam), anti-Foxp1 (CST), anti-Foxp2 (Sigma), anti-phospho-ERK1/2 (CST), and anti-ERK2 (Santa Cruz). An affinity-purified anti-MCRIP1 antibody was made in-house as described previously^{24 (link)}. All antibodies were used at a dilution of 1:1000 for western blotting. Full size western blot images are shown in Supplementary Fig. 6.

Tissues from neonatal lungs were isolated and samples were prepared for paraffin sections. Sections were stained with the following primary rabbit antibodies: anti-MCRIP1 (Atlas; 1:400); anti-SP-B (Santa Cruz; 1:300); anti-SP-C (Abcam; 1:1000), anti-CtBP1 (Abcam; 1:500) and anti-CtBP2 (Abcam; 1:500); anti-Foxp1 (CST; 1:200) and anti-Foxp2 (CST; 1:2000).

After separation in SDS-PAGE (AA: 10%, AA/BisAA ratio: 36:1), the proteins were transferred onto PVDF membranes following overnight incubation with specific primary antibodies. The following primary antibodies were used: anti-actin (Sigma A5441, Sigma-Aldrich, St. Louis, MO, USA), anti-Zeb1 (Sigma AMAb90510, Sigma-Aldrich, St. Louis, MO, USA), anti-Nanog (Santa Cruz sc-293121, Dallas, TX, USA), anti-Oct4 (Cell Signaling #2890s, Danvers, MA, USA), anti-Sox2 (Cell Signaling #3579s, Danvers, MA, USA), anti-CTBP2 (Abcam ab128871, Cambridge, UK), anti-CTBP1 (Sigma HPA018987, Sigma-Aldrich, St. Louis, MO, USA), anti-LSD1 (Sigma ABE365, Sigma-Aldrich, St. Louis, MO, USA), anti-TRIM33 (Sigma HPA004345, Sigma-Aldrich, St. Louis, MO, USA), and anti-p53 (Cell Signaling #46565, Danvers, MA, USA). The secondary antibodies were from Sigma: anti-mouse (A9917) and anti-rabbit (A0545). Bound antibodies were visualized using the SuperSignal West Femto Maximum Sensitivity Substrate ECL kit (Thermo scientific, Waltham, MA, USA), chemiluminescence was detected using ChemiDoc (BioRad, Hercules, CA, USA).