The fluorescence spectra were recorded on a Hitachi F-7100 spectrophotometer. The absorption spectra were recorded on a Shimadzu UV-3101PC spectrophotometer. A stock solution of NAF (0.017 mg ml−1) was prepared in acetonitrile–water. Colistin stock solutions (0.21 mg ml−1) and LPS stock solutions (1.718 mg ml−1) of different concentrations were prepared in Millipore water. The experiments were carried out in acetonitrile : water (2 : 5, v/v), buffered with 1 mM phosphate buffer (pH 7.0). During the titration, each time 35 μl of a 0.017 mg per ml solution of NAF was placed in a Starna quartz optical cell (sub-micro) of 3 mm optical path length, z = 15 mm, and then colistin and LPS solutions were added incrementally into the quartz optical cell using a micropipette. For a simultaneous discharge, premixed LPS–colistin solution was gradually added and titrated against NAF. The spectral data were recorded immediately after the addition of the colistin–LPS solution.
Uv 3101pc spectrophotometer
Manufactured by Shimadzu
Sourced in Japan
The UV-3101PC spectrophotometer is a versatile laboratory instrument designed for accurate and reliable UV-Vis spectroscopy measurements. It features a high-performance optical system and a wide wavelength range, making it suitable for a variety of applications in research and analytical laboratories.
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Lab products found in correlation
Smart apex 2 x ray single crystal diffractometer, by Bruker (2 mentions)
F 7100 spectrophotometer, by Hitachi (1 mentions)
Multimode 8 atomic force microscope, by Bruker (1 mentions)
400 mhz instrument, by Bruker (1 mentions)
R3310 02, by Hamamatsu Photonics (1 mentions)
S 4800, by Hitachi (1 mentions)
Jem 2100f, by JEOL (1 mentions)
Q tof micro mass spectrometer, by Waters Corporation (1 mentions)
Spectrum gx ft ir spectrophotometer, by PerkinElmer (1 mentions)
Avance 2 500 mhz, by JEOL (1 mentions)
Smart apex ccd diffractometer, by Bruker (1 mentions)
2400 chns o analyzer, by PerkinElmer (1 mentions)
Scmos camera, by Leica (1 mentions)
Isr 3100, by Shimadzu (1 mentions)
Avance 3, by Bruker (1 mentions)
Sigma 500, by Merck Group (1 mentions)
Jem 1400, by JEOL (1 mentions)
Multimode 8, by Bruker (1 mentions)
Flir e50, by Teledyne (1 mentions)
Tecnai g2 f20, by Thermo Fisher Scientific (1 mentions)
30 protocols using uv 3101pc spectrophotometer
1
Fluorescence Titration of NAF with Colistin and LPS
2
Organic Photovoltaic Characterization
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Absorption spectra of the organic films on quartz substrates were measured with a Shimadzu UV-3101PC spectrophotometer. The current density–voltage (J–V) characteristics of the OPV cells were measured using a computer-controlled Keithley 2611 source meter under AM 1.5 G illumination from a calibrated Solar with an irradiation intensity of 100 mW·cm−2. The external quantum efficiency (EQE) measurements were performed with a lock-in amplifier at a chopping frequency of 20 Hz during illumination with monochromatic light from a xenon lamp and their intensities are calibrated with a Si-photodiode. The surface topographies were imaged with a Bruker MultiMode 8 atomic force microscope (AFM) in tapping mode. The UPS experiments were performed using a VG ESCA Lab system equipped with a He I (21.22 eV) gas discharge lamp. The ultrahigh vacuum (UHV) system consists of a spectrometer chamber and an evaporation chamber. The base pressures of the spectrometer chamber and the evaporation chamber are typically 1.1 × 10−8 Pa and 6.7 × 10−6, respectively. We recorded the UPS spectra with the samples biased at −4.0 V to observe the true low energy secondary cut-off. The UV light spot size on samples was about 1 mm in diameter. The instrumental resolution for UPS measurements was chosen to be 10 meV. All the measurements were carried out at room temperature.
3
Analytical Characterization of Chemical Compounds
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Microanalyses (C, H, and N) were performed using a PerkinElmer 240Q elemental analyzer. Electrospray ionization mass spectroscopic (ESI–MS) analyses were performed using an LCQ system (Finnigan MAT, Waltham, MA, USA), where the mass spectra were recorded in positive mode and acetonitrile was used as the mobile phase. The spray voltage, tube lens offset, capillary voltage, and capillary temperature were set at 4.50 kV, 30.00 V, 23.00 V, and 200 °C, respectively, and the quoted m/z values were for the major peaks in the isotope distribution. 1H NMR and 13C NMR spectra were acquired at room temperature on a Varian-500 spectrometer (500 MHz) with DMSO-d6 as the solvent and tetramethylsilane (TMS) as an internal standard. UV-visible spectra were obtained on a Shimadzu UV-3101PC spectrophotometer. Fluorescence measurements were carried out on a Shimadzu RF-4500 fluorescence/phosphorescence spectrophotometer at room temperature.
4
Methanol Spectroscopic Analysis
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UV–Vis spectrum was recorded on UV-3101PC spectrophotometer of Shimadzu, Japan with solvent as methanol.
5
Fluorescence Spectra Measurement Protocol
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For compounds 8 , 9 , 17 , 18 and 24 fluorescence spectra (after excitation 405 nm) were measured in ethanol in quartz cuvettes (10 mm × 10 mm) on a Shimadzu UV-3101 PC spectrophotometer (Shimadzu, Japan) and on a Panorama fluorometer (Russia).
6
Rhodamine B Synthesis and Characterization
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Rhodamine B, phosphorus oxychloride and 3-amino phenol were purchased from Sigma-Aldrich Pvt. Ltd. (India). Unless mentioned otherwise, materials were obtained from commercial suppliers and were used without further purification. Solvents were dried according to the standard procedures. Elix Millipore water was used throughout all experiments. 1H and 13C NMR spectra were recorded on a Bruker 400 MHz instrument. For NMR spectra, DMSO-d6 and for NMR titration DMSO-d6 and D2O were used as solvent using TMS as an internal standard. Chemical shifts are expressed in δ ppm units and 1H–1H and 1H–C coupling constants in Hz. The mass spectrum (HRMS) was carried out using a micromass Q-TOF MicroTM instrument by using methanol as a solvent. Fluorescence spectra were recorded on a Perkin Elmer Model LS 55 spectrophotometer. UV spectra were recorded on a SHIMADZU UV-3101PC spectrophotometer. The following abbreviations are used to describe spin multiplicities in 1H NMR spectra: s = singlet; d = doublet; t = triplet; m = multiplet.
7
Characterization of CuGaO2 Nanoplates/ZnO Heterojunctions
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Field-emission scanning electron microscopy (FESEM, Hitachi S-4800), and high-resolution transmission electron microscopic (TEM, JEOL JEM-2100F) were used to study the surface morphology and microstructure of the samples. The absorption spectra were carried out using a Shimadzu UV-3101 PC spectrophotometer. Photoluminescence (PL) measurement was carried out with a JY-630 micro-Raman spectrometer employing the 325 nm line of a He-Cd laser as the excitation source. Low temperature PL measurements were acquired using the 325 nm line of a He-Cd laser and analyzed using a one meter grating spectrometer (SPEX 1704) equipped with a photomultiplier tube (Hamamatsu model R3310-02) cooled to 80 K. The current-voltage characteristics of the p-type CuGaO2 nanoplates/n-type ZnO film device were measured using a Keithley 2611A measurement system. The device was fabricated by assembling n-ZnO film (synthesized by sol-gel method on ITO substrate) with CuGaO2 nanoplates film (thermal evaporation of Au electrode on Si substrate followed by dropping CuGaO2 nanoplates).
8
Synthesis and Characterization of Organometallic Complexes
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All the chemicals were
purchased from Aldrich & Co. IR spectra were recorded using KBr
pellets (1% w/w) on a Perkin-Elmer Spectrum GX FT-IR spectrophotometer.
Electronic spectra were recorded on a Shimadzu UV 3101PC spectrophotometer.
Mass analyses were performed using the positive and negative ion spray
ionization technique on a Waters Q Tof-micro mass spectrometer for
all these complexes upon dissolving in methanol–water solvents.
CHNS analyses were done using a Perkin-Elmer 2400 CHNS/O analyzer.
Single crystal structures were determined using a BRUKER SMART APEX
(CCD) diffractometer. 1H and 13C NMR spectra
were recorded on a Bruker Avance II 500 MHz or Jeol 600 MHz FT-NMR
spectrometer. Chemical shifts for proton resonances are reported in
ppm (δ) relative to tetramethylsilane, and 13C spectra
are calibrated with reference to DMSO-d6. All the catalytic
products were established based on 1H NMR spectra. The
100 mL high-pressure catalytic reactor was from AMAR equipment.
purchased from Aldrich & Co. IR spectra were recorded using KBr
pellets (1% w/w) on a Perkin-Elmer Spectrum GX FT-IR spectrophotometer.
Electronic spectra were recorded on a Shimadzu UV 3101PC spectrophotometer.
Mass analyses were performed using the positive and negative ion spray
ionization technique on a Waters Q Tof-micro mass spectrometer for
all these complexes upon dissolving in methanol–water solvents.
CHNS analyses were done using a Perkin-Elmer 2400 CHNS/O analyzer.
Single crystal structures were determined using a BRUKER SMART APEX
(CCD) diffractometer. 1H and 13C NMR spectra
were recorded on a Bruker Avance II 500 MHz or Jeol 600 MHz FT-NMR
spectrometer. Chemical shifts for proton resonances are reported in
ppm (δ) relative to tetramethylsilane, and 13C spectra
are calibrated with reference to DMSO-d6. All the catalytic
products were established based on 1H NMR spectra. The
100 mL high-pressure catalytic reactor was from AMAR equipment.
9
Absorption Spectroscopy of Samples
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Electronic absorption spectra were obtained using a Shimadzu UV-3101PC spectrophotometer in the range of 350–800 nm in a quartz cuvette (optical path length 1 cm). Molar extinction coefficients were used from [25 (link)].
10
Temperature-Controlled UV-VIS Spectroscopy
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UV–VIS spectra were recorded with a Shimadzu UV-3101 PC spectrophotometer (Shimadzu, Kyoto, Japan), and a LINKAM TP-93 heating stage (LINKAM, Tadworth, UK) was used for temperature control. Samples were deposited on standard microscope cover slides (0.17 mm thick). A Hamamatsu LC8n light source (Hamamatsu, Hamamatsu City, Japan) was used for the UV irradiation.
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