KCl (Sigma-Aldrich, Saint Louis, MO, USA) and ultrapure water obtained using a Milli-Q Simplicity® Water Purification System (Merk, Darmstadt, Germany) were used to prepare 0.1 M KCl solution. This was used as reference solution for checking the sensor signal before recording the signals in the water samples to be analyzed.
Potassium chloride (kcl)
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, India, France, Spain, China, Belgium, Sao Tome and Principe, Canada, Denmark, Poland, Australia, Ireland, Israel, Singapore, Macao, Switzerland, Brazil, Mexico, Hungary, Netherlands, Egypt, Japan, Sweden, Indonesia, Czechia, Chile
Potassium chloride (KCl) is an inorganic compound that is commonly used as a laboratory reagent. It is a colorless, crystalline solid with a high melting point. KCl is a popular electrolyte and is used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (781 mentions)
Sodium hydroxide (naoh), by Merck Group (256 mentions)
Hydrochloric acid (hcl), by Merck Group (253 mentions)
Mgcl2, by Merck Group (220 mentions)
Cacl2, by Merck Group (206 mentions)
Kh2po4, by Merck Group (201 mentions)
Nahco3, by Merck Group (170 mentions)
Hepes, by Merck Group (145 mentions)
Methanol, by Merck Group (135 mentions)
Calcium chloride, by Merck Group (121 mentions)
Na2hpo4, by Merck Group (118 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (110 mentions)
Potassium dihydrogen phosphate, by Merck Group (109 mentions)
Sucrose, by Merck Group (109 mentions)
Bovine serum albumin (bsa), by Merck Group (109 mentions)
Ethanol, by Merck Group (103 mentions)
Acetic acid, by Merck Group (90 mentions)
Sodium bicarbonate, by Merck Group (84 mentions)
Sodium acetate, by Merck Group (80 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (80 mentions)
1 988 protocols using potassium chloride (kcl)
1
Preparation of 0.1 M KCl Reference Solution
2
Synthesis of Ferrocenyl Ammonium Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ferrocenylmethyl trimethylammonium hexafluorophosphate, [FcTMA + ][PF 6 -] was synthesized in-house via an exchange reaction of FcTMA + I -(Strem Chemicals, Ltd.) with AgPF 6 (Strem Chemicals, Ltd.). KCl (99 %) was purchased from Sigma-Aldrich and used without further purification. All solutions were prepared using high purity water (Millipore Corp. purification system), with a resistivity ca. 18.2 MΩ cm at 25 o C. 1 M KCl was added as the supporting electrolyte in all solutions.
3
Synthesis of Hafnium Boride and Calcium Hexaboride
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synthesis of the nanoscaled precursors. Before heating, all powders were handled into an argon-filled glovebox. Anhydrous HfCl4 (Alfa Aesar, 99.9 %), CaCl2 (Alfa Aesar, 99.9 %) and NaBH4 (Alfa Aesar, 98 %) were used as received. Prior to synthesis, LiCl and KCl (Aldrich) were mixed at the eutectic composition LiCl:KCl = 45:55 wt% and finely ground in a mortar. The resulting mixture was evacuated at 200 °C for 4 days and transferred into the glovebox. Before heating, anhydrous metal chlorides, sodium borohydride and the eutectic salt mixture LiCl:KCl (2.5 g) were finely ground together with a Retsch MM400 ballmiller (airtight vials of 50 mL, one steel ball of 62.3 g and a diameter of 23 mm) for 2 min at 20 Hz. The mixture was transferred into a glassy carbon crucible which was then heated under argon flow in a tube oven at 10 °C min -1 . For HfB2, 1 mmol of hafnium (IV) chloride and 4 mmol of sodium borohydride were used. The reaction medium was heated at 900 °C for 4 h. For CaB6, 1 mmol of calcium (II) chloride and 8 mmol of sodium borohydride were used. The reaction medium was heated at 800 °C for 4 h. After cooling, the metal boride powders were recovered by dissolution of the frozen eutectic in deionized water and four washingcentrifugation cycles (in 10 mL polycarbonate centrifugation tubes, at 16500 rpm for 20 min), then dried under vacuum at 60 °C overnight.
4
Pulp Purification and Chemical Reagent Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Never dried NBSK pulp was supplied by Canfor Pulp Products. The NBSK pulp was washed with deionized water until the UV–vis absorption of filtrate was less than 0.005 abs at 200 nm wavelength before use. LBG with purity greater than 90% was purchased from Sigma Aldrich. Sodium chloride (> 99%), potassium chloride (> 99%), hydrochloric acid (37%), sodium acetate (> 99%), acetic acid (> 99%), sodium bicarbonate (> 99%), sodium carbonate (> 99%), potassium chloride (> 99%) and sodium hydroxide (> 98%) were purchased from Sigma Aldrich. Sodium phosphate monobasic (> 98%) and dibasic (> 99%) were purchased from Fisher Scientific. Sulfuric acid (98 wt%) was purchased from Sigma Aldrich and diluted to desired concentration. The carbohydrates kit (CAR10-1KT) used to calibrate the high-performance liquid chromatography (HPLC) was purchased from Sigma Aldrich and contained mannose, glucose, galactose, xylose and arabinose. The purity of the standards was greater than 98%.
5
Molecular Beacon-Based Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MBs and miRNA-21 oligonucleotide were purchased from Sigma-Aldrich, UK. KCl, MgCl2 and Tris–HCl were also purchased from Sigma-Aldrich, UK. The MBs have a 5′ 6-FAM fluorophore and 3′ Black Hole Quencher-1 (BHQ-1),32 which quenches 6-FAM fluorescence. The structure of the designed MBs is depicted in Fig. 2A .
The two engineered MB constructs (Cy3 labelled), their complementary and control sequences and the peptide KAMHAWGCGGGC-NH2 have been purchased from Biosynthesis (Texas, USA). The salts used to make PBS (sodium chloride, potassium chloride, sodium phosphate dibasic heptahydrate and potassium phosphate monobasic) were purchased from Sigma-Aldrich (Missouri, USA).
The two engineered MB constructs (Cy3 labelled), their complementary and control sequences and the peptide KAMHAWGCGGGC-NH2 have been purchased from Biosynthesis (Texas, USA). The salts used to make PBS (sodium chloride, potassium chloride, sodium phosphate dibasic heptahydrate and potassium phosphate monobasic) were purchased from Sigma-Aldrich (Missouri, USA).
6
Measuring Intracellular Calcium Dynamics in Cardiospheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In one set of experiments, after holo-f-aequorin reconstitution, the background level of luminescence was recorded for a period of 160 min. Data acquisition was then paused briefly while 200 μL PBS containing 5% Triton X-100 was added to the tube containing the cardiospheres and luminescence recording was resumed. The Triton X-100 was used to permeabilize the cardiosphere cells and thus expose any unspent holo-f-aequorin to extracellular Ca 2+ . This confirmed that the low level of aequorin-generated luminescence (and thus the [Ca 2+ ]) observed was real and not due to the Ca 2+ reporter being used up (Leung et al., 2009) .
In another series of experiments, after holo-f-aequorin reconstitution, the background level of luminescence was recorded for ~1 h, after which data acquisition was paused briefly while 200 μL KCl (Sigma-Aldrich; at 20 mmol L 1 in bathing medium) was added to the luminometer tube containing the cardiospheres and recording was resumed. The effect of KCl on the aequorin-generated luminescence was measured for ~5 min, after which in some cases the cardiospheres were treated with a second dose of 20 mmol L 1 KCl. Cardiospheres were also treated with 200 μL CaCl 2 (Sigma-Aldrich; at 1 mmol L 1 in bathing medium), using a similar protocol to that described for the KCl.
In another series of experiments, after holo-f-aequorin reconstitution, the background level of luminescence was recorded for ~1 h, after which data acquisition was paused briefly while 200 μL KCl (Sigma-Aldrich; at 20 mmol L 1 in bathing medium) was added to the luminometer tube containing the cardiospheres and recording was resumed. The effect of KCl on the aequorin-generated luminescence was measured for ~5 min, after which in some cases the cardiospheres were treated with a second dose of 20 mmol L 1 KCl. Cardiospheres were also treated with 200 μL CaCl 2 (Sigma-Aldrich; at 1 mmol L 1 in bathing medium), using a similar protocol to that described for the KCl.
7
Synthesis of Gel-Type Internal Electrolyte
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The synthesis of the gel-type internal electrolyte is described as follows. First, 3.5 mol/L potassium chloride (KCl; 99.0%, Sigma-Aldrich, USA) solution was prepared and heated at 60°C. Then, 8 ml of glycerol (>99.5%, Sigma-Aldrich, USA) was poured into the KCl solution, which played an important role in preventing the high-density gelation of internal electrolyte. Finally, 5 wt.% of hydroxyethyl cellulose (HEC, 200–400 mPa·s, DaeJung Co., Ltd., South Korea) was slowly added into the heated KCl solution (100 ml KCl solution with 5 g HEC) with constant stirring at 150 rpm, affording the HEC-gelled electrolyte. The conductivity of the gel-type electrolyte was 226 mS/cm at 5 wt.% HEC and that of 3.5 M KCl solution was 294.8 mS/cm (Kim et al., 2017 (link)). This indicated that the gel-type electrolyte could decrease the migrating rate of ions, which further decreased the rates of electrolyte loss and exterior ions entering into the internal electrolyte. Furthermore, HEC showed good water absorptivity. Using the HEC-based gel-type electrolyte decreased the rate of moisture loss, thus prolonging the life time of RE in the RC structure.
8
Hypothalamic Slice Preparation for Neuron Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The brain slices of hypothalamic arc region were obtained from POMC-EGFP, AgRP-Cre-Ai14, and DAT-Cre-Ai14 mice (postnatal age: 4–6 weeks). Mice were deeply anesthetized with isoflurane shortly before brain slicing. The brains were quickly shift into cold (4 °C), oxygenated (5% CO2, 95% O2) slicing medium containing 110 mM of choline chloride (Sigma-Aldrich), 2.5 mM of KCl (Sigma-Aldrich), 1.2 mM of NaH2PO4 (Sigma-Aldrich), 25 mM of NaHCO3 (Sigma-Aldrich), 20 mM of glucose (Sigma-Aldrich), 7 mM of MgCl2 (Sigma-Aldrich), and 0.5 mM of CaCl2 (Sigma-Aldrich). Coronal slices (150 μm) were cut using a vibratome. After slicing, the mice brain slices were transferred to a holding chamber filled with oxygenated (5% CO2, 95% O2) artificial CSF (ACSF) solution containing 124 mM of NaCl (Sigma-Aldrich), 2.5 mM of KCl, 1 mM of NaH2PO4, 26.2 mM of NaHCO3, 20 mM of glucose, 1.3 mM of MgCl2, and 2.5 mM of CaCl2. After at least one hour of recovery, individual slices were transferred to a recording chamber. Oxygenated ACSF continuously flew at a rate of 5 mL/min at 30–32 °C temperature.
9
Heavy Metal Analysis in Environmental Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents and chemicals used in this study were analytical grade, unless otherwise stated. Double distilled water was used for all preparation and dilution purposes of solutions throughout the experimental procedures. Chemicals such as HNO3 (69%), ammonium acetate (≥98%), sodium acetate (≥99%), KCl (≥99%), HAc (≥99%), MgCl2 (≥99%), NH2OH.HCl (98%), H2SO4 (98%) and H2O2 (30%) and HCl (37%) (all from Sigma Aldrich, USA) were used during sample digestion procedures. Stock standard solutions of 1000 ppm were prepared from their corresponding salts for the selected heavy metals (Cu, Zn, As, Cr, Fe, Mn, Ni, Pb, Cd, Hg and Co). Standard buffer solutions of pH = 4, 7 and 9 (from Macron Fine Chemicals™) were used for pH meter calibration and KCl (from Sigma Aldrich, USA) was used for conductivity meter calibration.
10
Synthesis of Eutectic AgCl-KCl Infiltration
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Preparation of the eutectic AgCl–KCl involved mixing as-received AgCl (99.999%; Sigma Aldrich) and KCl (99.99%; Sigma-Aldrich) as per the eutectic composition (70 mol % AgCl and 30 mol % KCl), followed by melting in a glass vial at 470 °C for 2 h in a tube furnace. The air-cooled eutectic was broken into small pieces using agate mortar and pestle. A small piece (∼80 mg) was placed on top of the 3D mesostructure. The setup was heated to 450 °C on a Linkam THMS600 hot stage and held at that temperature for 5 min. The eutectic melted and flowed down along the ribbons of the 3D mesostructure. Deactivating the hot stage resulted in cooling at a rate of ∼140 °C/min, allowing the solidification to occur from top to bottom.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!