Log In Sign up
The largest database of trusted experimental protocols

Cm1032

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in France

The CM1032 is a laboratory centrifuge designed for general purpose applications. It features a robust construction and can accommodate a variety of sample tubes and microplates. The centrifuge provides consistent and reliable performance to meet the needs of various laboratory workflows.

Automatically generated - may contain errors

Lab products found in correlation

Cm0509, by Thermo Fisher Scientific (2 mentions) Bhi broth, by Thermo Fisher Scientific (1 mentions) Unimax 2010, by Heidolph (1 mentions) Rapid e coli 2 agar plates, by Bio-Rad (1 mentions) Lp0011, by Thermo Fisher Scientific (1 mentions)

4 protocols using cm1032

1

Bacterial Strains Preparation and Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two bacterial strains were used for this study, S. aureus ATCC 29213 and P. aeruginosa S0599 (internal reference). These bacteria were stored at −80 °C in glycerol-BHI (VWR 24388.295; Brain Heart Infusion, Oxoid CM1032). Bacterial strains were revived by inoculating 10 µl into BHI broth (Oxoid) and incubated at 37 °C overnight and under agitation (Unimax 2010; Heidolph) to obtain 108–109 CFU ml−1. Ten microlitres of the solution was spread on plate count agar (PCA, 3564475; Bio-Rad) to control the purity of the bacterial strains and the absence of contamination.
Martinez E., Crèvecoeur S., Dams L., Rabecki F., Habraken S., Haubruge E, & Daube G. (2022). Effect of five decontamination methods on face masks and filtering facepiece respirators contaminated with Staphylococcus aureus and Pseudomonas aeruginosa. Access Microbiology, 4(3), 000342.
+ Open protocol
+ Expand
2

Enrichment and Isolation of E. coli from Swab Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
On the same day, each swab sample was enriched with 10 mL of Buffered Peptone Water (BPW, Oxoid, CM0509, Basingstoke, Hampshire, England), homogenized by a Masticator (IUL instruments, S.A., Barcelona, Spain) and incubated for 24 h at 37 °C for the detection of E. coli. After incubation, 10 μL of the BPW fraction was plated on Rapid’E. coli 2 agar plates (Bio-Rad, 356–4024, Marnes-la-Coquette, France) and incubated at 44 °C for 24 h. From each positive Rapid’E. coli 2 plate one isolate was purified and stored at − 80 °C on brain heart infusion (BHI, Oxoid, CM1032) supplemented with 15% (v/v) glycerol. In total, 67 and 183 E. coli isolates were obtained during the longitudinal study at the broiler pilot farm and the experimental pig farm, respectively.
Maertens H., Van Coillie E., Millet S., Van Weyenberg S., Sleeckx N., Meyer E., Zoons J., Dewulf J, & De Reu K. (2020). Repeated disinfectant use in broiler houses and pig nursery units does not affect disinfectant and antibiotic susceptibility in Escherichia coli field isolates. BMC Veterinary Research, 16, 140.
+ Open protocol
+ Expand
3

Culturing Fusobacterium nucleatum

Check if the same lab product or an alternative is used in the 5 most similar protocols
F. nucleatum (ATCC 10953) was grown on heart infusion (HI) agar, containing HI broth (Oxoid, CM1032, Thebarton, SA, Australia), 0.5% haemin (Sigma-Aldrich, 51280, Bayswater, VIC, Australia) and 1% bacteriological agar (Oxoid, LP0011, Thebarton, SA, Australia). The culture was grown in anaerobic conditions using anaerobic generating sachets (AnaeroGenTM 2.5L, AN0025A, Oxoid, Thebarton, SA, Australia) at 37 °C for 48 h.
Binte Mohamed Yakob Adil S.S., Kabwe M., Cianciarulo C., Nguyen T.H., Irving H, & Tucci J. (2023). IRAK3 Knockout and Wildtype THP-1 Monocytes as Models for Endotoxin Detection Assays and Fusobacterium nucleatum Bacteriophage FNU1 Cytokine Induction. International Journal of Molecular Sciences, 24(20), 15108.
+ Open protocol
+ Expand
4

Rapid E. coli Isolation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
After sampling, swabs were transported to the lab in a cool box with ice packs. Upon their arrival in the lab (± 2 h after sampling), 10 mL of Buffered Peptone Water (BPW, Oxoid, CM0509, Basingstoke, Hampshire, England) was immediately added to each sample, homogenized by a Masticator (IUL instruments, S.A., Barcelona, Spain) and incubated for 24 h at 37 °C for enrichment of E. coli. After incubation, 10 μL of the enriched BPW fraction was plated on Rapid’E. coli 2 agar plates (Biorad, 356–4024, Marnes-la-Coquettes, France) and incubated at 44 °C for 24 h. From positive Rapid’E. coli 2 plates purified isolates were obtained and stored at − 80 °C on brain heart infusion (BHI, Oxoid, CM1032) supplemented with 15% (v/v) glycerol.
Maertens H., De Reu K., Meyer E., Van Coillie E, & Dewulf J. (2019). Limited association between disinfectant use and either antibiotic or disinfectant susceptibility of Escherichia coli in both poultry and pig husbandry. BMC Veterinary Research, 15, 310.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!