Gsk690693

Manufactured by Merck Group

Sourced in United States

GSK690693 is a laboratory research product distributed by Merck Group. It is a chemical compound used for research purposes. Further details on the core function or intended use of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

12 protocols using gsk690693

Evaluating CXCL16/CXCR6 Expression in ESCs, DSCs, and Trophoblast Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To evaluate the expression of CXCL16/CXCR6 in ESCs, DSCs or trophoblast cells, ESCs and DSCs were seeded into 24-well plates (2 × 10⁵ cells/well) and incubated in DMEM/F-12 medium containing 10% v/v FBS; trophoblast cells were in DMEM medium (Hyclone) containing 20% v/v FBS. The medium was changed every 3 days. The cells (1, 3, 6 and 9 days) were collected for flow cytometry. The culture supernatants (1, 3, 6, and 9 days) were also collected and centrifuged to remove cellular debris and stored at −80°C for IGFBP-1 and PRL measurements.
In addition, to clarify the effect of CXCL16 on ESCs decidualization, decidualised ESCs (ESCs incubated with cAMP plus MPA for 6 days) were then treated with 100 ng/mL recombinant human CXCL16 (rhCXCL16; Cell Signaling Technology), or 5 µg/mL CXCL16 antagonist (anti-CXCL16; Cell Signaling Technology) or the culture supernatants of trophoblast cells, or with culture media of trophoblast (high-glucose DMEM containing 20% v/v FBS), or with vehicle (0.1% v/v dimethyl sulfoxide; Sigma) as control for 3 days. Additionally, the PI3K-AKT signalling pathway inhibitor 10 µM GSK 690693 (Sigma) was added in the past 24 h.

Mei J., Yan Y., Li S.Y., Zhou W.J., Zhang Q., Li M.Q, & Sun H.X. (2019). CXCL16/CXCR6 interaction promotes endometrial decidualization via the PI3K/AKT pathway. Reproduction (Cambridge, England), 157(3), 273-282.

+ Open protocol

+ Expand

Investigating PDGF-BB Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

All pharmacological compounds were prepared as aqueous or dimethyl sulfoxide stock solutions of >1,000 times the concentration used during the experiment. Recombinant mouse PDGF-BB was purchased from BioLegend (San Diego, CA, USA). AG1296, AG1295, GSK690693, and forskolin were purchased from Sigma (St. Louis, MO, USA). Rp-8-CPT-cAMPs and SQ22536 were purchased from BIOLOG Life Science Institute (La Jolla, CA, USA).

Tang C., Wang D., Luo E., Yan G., Liu B., Hou J, & Qiao Y. (2020). Activation of Inward Rectifier K+ Channel 2.1 by PDGF-BB in Rat Vascular Smooth Muscle Cells through Protein Kinase A. BioMed Research International, 2020, 4370832.

+ Open protocol

+ Expand

FOXO3a Relocalisation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Leptomycin B was purchased from NEB. PI-103 and BEZ235 were purchased from Selleckchem. GSK690693 was purchased from Sigma. GSK2334470 was purchased from Stratech Scientific Ltd. LY294002 was purchased from Merck Millipore. All compounds were resuspended in DMSO with the exception of Leptomycin B which was supplied as a solution in ethanol. Compounds were serially diluted in medium using a 2-fold dilution series, DMSO was kept constant with a maximum final concentration of 0.2%. Cells were plated in 96-well plates and treated the following day in triplicate with diluted compounds for 3 hr. After 3 hr, cells were fixed and stained according to the FOXO3a Relocalisation Assay protocol.

Emery A., Hardwick B.S., Crooks A.T., Milech N., Watt P.M., Mithra C., Kumar V., Giridharan S., Sadasivam G., Mathivanan S., Sudhakar S., Bairy S., Bharatham K., Hurakadli M.A., Prasad T.K., Kamariah N., Muellner M., Coelho M., Torrance C.J., McKenzie G.J, & Venkitaraman A.R. (2021). Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library. Cell Chemical Biology, 28(11), 1602-1615.e9.

+ Open protocol

+ Expand

CX3CL1 and IL-6 Signaling Pathway Modulators

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human CX3CL1 was obtained from Peprotech. Recombinant human IL-6 protein was obtained from R&D systems. Acriflavine (HIF-1 inhibitor), KN-93 (CaMKII inhibitor), GSK690693 (pan-Akt inhibitor), BAY 11-7082 (NF-κB signaling inhibitor), U73122 hydrate (pan-PLC inhibitor), Stattic (STAT3 inhibitor), LY-294002 hydrochloride (PI3K inhibitor), Sodium oxamate (LDHA inhibitor), pertussis toxin (PTx,Gα_i/0 inhibitor), H-89 (PKA/ROCK inhibitor), Y-27632 (ROCK inhibitor) and CoCl₂ were all obtained from Sigma-Aldrich. Rapamycin (mTORC1 inhibitor) was purchased from Cayman Chemical. 17-AAG (HSP90 inhibitor) and P6 (pan-JAK inhibitor), Bisindolylmaleimide I (PKC inhibitor) were purchased from Calbiochem. The Gα_q-inhibitor UBO-QIC was purchased from the Institute of Pharmaceutical Biology, University of Bonn. Gallein (βγ-subunit inhibitor) was obtained from Tocris.

de Wit R.H., Mujić-Delić A., van Senten J.R., Fraile-Ramos A., Siderius M, & Smit M.J. (2016). Human cytomegalovirus encoded chemokine receptor US28 activates the HIF-1α/PKM2 axis in glioblastoma cells. Oncotarget, 7(42), 67966-67985.

+ Open protocol

+ Expand

Inhibition of Pancreatic Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mouse pancreatic cancer cell lines PanIN and IPMN were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Nacalai, Japan) supplemented with 10% heat-inactivated fetal bovine serum (HyClone, USA) and 100 IU penicillin–streptomycin/ml (Nacalai, Japan). The Akt inhibitor GSK690693 (Sigma, USA) and gemcitabine (Tokyo Chemical Industry Co., Japan) were added to the cultures on the second day after seeding. Briefly, the cells were cultured with a fixed concentration of GSK690693 or AZD4547 for the next 4 days.

Chen R., Sugiyama A., Kataoka N., Sugimoto M., Yokoyama S., Fukuda A., Takaishi S, & Seno H. (2020). Promoter-Level Transcriptome Identifies Stemness Associated With Relatively High Proliferation in Pancreatic Cancer Cells. Frontiers in Oncology, 10, 316.

+ Open protocol

+ Expand

Molecular Mechanisms of 20(S)-GRh2 in Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

20(S)-GRh2, with a purity of 99.48%, was obtained from Beijing North Carolina Chuanglian Biological Technology Research Institute (Beijing, China). Working stock solutions of 20(S)-GRh2 were prepared by dissolving compounds in dimethyl sulfoxide (DMSO; Sigma-Aldrich, Milan, Italy) before use. The final density of DMSO was < 0.1%. LY294002 (PI3K inhibitor), GSK690693 (Akt inhibitor), and Rapamycin (mTOR inhibitor) were obtained from Sigma (St. Louis, MO, USA).
The primary antibodies against cytochrome c, cleaved caspase-3, Beclin-1, Atg5, cyclin B1, and cyclin D1 and the secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). β-Actin, Bax, Bal-2, LC3, p-P70S6K, and p-4EBP1 were purchased from the Abcam company (Cambridge, MA, USA). PI3K (p85), Akt, p-Akt (Ser473), mTOR, and p-mTOR (Ser2448) were derived from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The primary antibody against human CD3 was purchased from eBioscience (San Diego, CA, USA).

Xia T., Zhang J., Zhou C., Li Y., Duan W., Zhang B., Wang M, & Fang J. (2019). 20(S)-Ginsenoside Rh2 displays efficacy against T-cell acute lymphoblastic leukemia through the PI3K/Akt/mTOR signal pathway. Journal of Ginseng Research, 44(5), 725-737.

+ Open protocol

+ Expand

Cell Culture and Pharmacological Inhibition

Check if the same lab product or an alternative is used in the 5 most similar protocols

5637, BIU87, T24 and EJ cells were obtained from the American Type Culture Collection (Manassas, VA, USA) and cultured at 37 °C in 5% CO₂ in DMEM-F12 (1:1) media (Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 1% penicillin (100 U/ml, Invitrogen Life Technologies), 1% streptomycin (100 μg/ml, Invitrogen Life Technologies), L-glutamine (292 μg/ml, Invitrogen Life Technologies) and 10% fetal bovine serum (HyClone Laboratories, Logan, UT, USA). GSK690693 (Sigma-Aldrich, St. Louis, MO, USA; 5 μM) or MK-2206 (Selleckchem, Houston, TX, USA; 5 μM) was added to the media at the indicated times. For transient gene transfection of cells, Lipofectamine 2000 (Invitrogen Life Technologies) was used.

Jiang L., Dong P., Zhang Z., Li C., Li Y., Liao Y., Li X., Wu Z., Guo S., Mai S., Xie D., Liu Z, & Zhou F. (2015). Akt phosphorylates Prohibitin 1 to mediate its mitochondrial localization and promote proliferation of bladder cancer cells. Cell Death & Disease, 6(2), e1660-.

+ Open protocol

+ Expand

Hedgehog Signaling Modulator Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Smoothened agonist SAG was purchased from Calbiochem. SANT (SANT-1); KU-0063794, GSK-690693, PF-4708671 and MG132 were from Sigma and AZ191 was from SelleckChem. Cycloheximide was purchased from Biomol.
The monoclonal Hedgehog neutralizing antibody (5E1, supernatant), developed/deposited by T.M. Jessell/S. Brenner-Morton was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242.

Singh R., Dhanyamraju P.K, & Lauth M. (2016). DYRK1B blocks canonical and promotes non-canonical Hedgehog signaling through activation of the mTOR/AKT pathway. Oncotarget, 8(1), 833-845.

+ Open protocol

+ Expand

Small Molecule Inhibitor Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dasatinib, Torin 1, and rapamycin were obtained from LC Laboratories (Boston, MA). PP242 and RAD001 were obtained from Selleckchem (Houston, TX). PP2, U0126, and GSK 690693 were obtained from Sigma-Aldrich (St. Louis, MO). GSK 650394 was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). LY294002 was obtained from Cell Signaling (Danvers, MA). CGP 57380 was obtained from Abcam (Cambridge, MA).

Broeckel R., Sarkar S., May N.A., Totonchy J., Kreklywich C.N., Smith P., Graves L., DeFilippis V.R., Heise M.T., Morrison T.E., Moorman N, & Streblow D.N. (2019). Src Family Kinase Inhibitors Block Translation of Alphavirus Subgenomic mRNAs. Antimicrobial Agents and Chemotherapy, 63(4), e02325-18.

+ Open protocol

+ Expand

Regulatory Signaling Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

K1-plasmid YAP1 cells were grown as described above until they became 40% confluent. Then, the media were replaced with RPMI1640 containing vehicle (0.1% (v/v) dimethyl sulphoxide), U0126 (10 μM) (Promega, Madison, WI) or GSK690693 (10 μM) (Sigma Chemical Co., St. Louis, MO). Samples were collected at different time intervals as indicated for each experiment.

Liao T., Wen D., Ma B., Hu J.Q., Qu N., Shi R.L., Liu L., Guan Q., Li D.S, & Ji Q.H. (2016). Yes-associated protein 1 promotes papillary thyroid cancer cell proliferation by activating the ERK/MAPK signaling pathway. Oncotarget, 8(7), 11719-11728.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!