Rat interleukin (IL)-10, IL-1α, interferon-gamma (IFN-γ), and tumor necrosis factor α (TNFα) protein levels were quantitatively measured by cytometric bead array flex sets (CBA) (BD Biosciences, San Diego, CA, USA) according to the manufacturer’s protocol. Rat CXCL2, IL-1β, leptin and C-reactive protein (CRP) (R&D Systems, Minneapolis, MN, USA), IL-6 (BioLegend, London, UK), lipopolysaccharide (LPS) binding protein (LBP) (Hycult Biotech, Uden, The Netherlands), and insulin (Crystal Chem Zaandam, The Netherlands) were quantified by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol.
Lipopolysaccharide lps binding protein lbp
Manufactured by Hycult Biotech
Sourced in United Kingdom
Lipopolysaccharide (LPS) binding protein (LBP) is a laboratory product that functions as a carrier protein. It binds to the lipopolysaccharide component of the outer membrane of Gram-negative bacteria. This binding facilitates the transfer of LPS to CD14, a receptor that plays a role in the immune response to bacterial infections.
Automatically generated - may contain errors
Lab products found in correlation
Cxcl2, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by BioLegend (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Insulin, by Crystal Chem (1 mentions)
Salmonella typhimurium, by InvivoGen (1 mentions)
Endocab, by Hycult Biotech (1 mentions)
Soluble cd14 scd14, by R&D Systems (1 mentions)
Fabp2, by R&D Systems (1 mentions)
2 protocols using lipopolysaccharide lps binding protein lbp
1
Quantifying Inflammatory Biomarkers in Rats
2
Serological Markers of Celiac Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Established serological markers of coeliac disease, including IgA antibody to TG2 and IgG and IgA antibodies to deamidated gliadin, were measured as previously described.24 (link)
25 (link)
Serum IgG, IgA and IgM antibodies to native gliadin were measured separately by ELISA as previously described,24 (link)
26 (link) with the following modification: the secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-human IgG (GE Healthcare), IgA (MP Biomedicals) or IgM (MP Biomedicals). Serum IgG, IgA and IgM antibodies to bacterial flagellin were measured separately using a similar protocol for detecting antibodies to gliadin, with the following modification: plates were coated with a 2 μg/mL solution of highly purified flagellin from Salmonella typhimurium (InvivoGen).
Levels of serum IgG, IgA and IgM endotoxin-core antibodies (EndoCAb) (Hycult Biotech), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems) and fatty acid-binding protein 2 (FABP2) (R&D Systems) were determined by ELISA, according to the manufacturers' protocols.
25 (link)
Serum IgG, IgA and IgM antibodies to native gliadin were measured separately by ELISA as previously described,24 (link)
26 (link) with the following modification: the secondary antibodies were horseradish peroxidase (HRP)-conjugated anti-human IgG (GE Healthcare), IgA (MP Biomedicals) or IgM (MP Biomedicals). Serum IgG, IgA and IgM antibodies to bacterial flagellin were measured separately using a similar protocol for detecting antibodies to gliadin, with the following modification: plates were coated with a 2 μg/mL solution of highly purified flagellin from Salmonella typhimurium (InvivoGen).
Levels of serum IgG, IgA and IgM endotoxin-core antibodies (EndoCAb) (Hycult Biotech), lipopolysaccharide (LPS)-binding protein (LBP) (Hycult Biotech), soluble CD14 (sCD14) (R&D Systems) and fatty acid-binding protein 2 (FABP2) (R&D Systems) were determined by ELISA, according to the manufacturers' protocols.
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