Caludin 1

Antibodies for p65, p-p65, BDNF, claudin-5, occludin, caludin-1, iNOS, COX-2, and β-actin were purchased from Cell Signaling Technology (Beverly, MA). Enzyme-linked immunosorbent assay (ELISA) kits for cytokines were purchased from Ebioscience (San Diego, CA). 4′,6-Diamidino-2-phenylindole dilactate (DAPI) was purchased from Sigma (St. Louis, MO). QIAamp Fast DNA stool mini kit was purchased from Qiagen (Hilden, Germany). Limulus amoebocyte lysate (LAL) assays was purchased from Cape Cod Inc. (East Falmouth, MA). General anaerobic medium (GAM), BL, and DHL media were from Nissui Pharmaceutical Co. (Tokyo, Japan). MRS medium was purchased from BD (Radnor, PA).

The cell lysates preparation using the methods as previously described (15 (link), 16 (link)). The cell extracts were separated by 8 or 10% polyacrylamide gel and transferred onto a polyvinylidene fluoride (PVDF) membrane. The membrane was blocked in TBS-T buffer (20 mM Tris, 137 mM NaCl, pH 7.4) with 5% nonfat milk for 1 h, and the blots were probed with the indicated primary antibodies for 24 h at 4°C. After washing steps with TBS-T (10 min each) for three times, the blots were incubated with horseradish peroxidase anti-mouse IgG or goat anti-rabbit for 1 h at room temperature. Last, the blots were visualized by ECL reagent (Millipore), and the protein expression was detected by chemiluminescence. The relative photographic density was quantified by ImageQuant LAS 4000 mini Biomolecular Imager (GE Healthcare Bio-Sciences AB, Björkgatan 30 751 84 Uppsala, Sweden). EMT antibodies for E-cadherin [(24E10) Rabbit mAb #3195], Vimentin [(D21H3) XP® Rabbit mAb #5741], TCF8/ZEB1 [(D80D3) Rabbit mAb #3396], Snail-1 [(Rabbit mAb #3879), Slug [(C19G7) Rabbit mAb #9585], Caludin-1 [(D5H1D) XP® Rabbit mAb #13255], β-catenin [(D10A8) XP^® Rabbit mAb #8480] were obtained from Cell Signaling; antibodies for EMT were obtained from Cell Signaling EMT antibody sampler kit #9782. Twist (GTX127310) antibody was obtained from GeneTex (Hsinchu City, Taiwan).

Enzyme-linked immunosorbent assay (ELISA) kits for corticosterone and cytokines were purchased from Ebioscience (San Diego, CA). 4′,6-Diamidino-2-phenylindole dilactate (DAPI) was purchased from Sigma (St. Louis, MO). Antibodies for p65, p-p65, BDNF, claudin-5, occludin, caludin-1, iNOS, COX-2, and β-actin were purchased from Cell Signaling Technology (Beverly, MA). QIAamp Fast DNA stool mini kit was purchased from Qiagen (Hilden, Germany). Limulus amoebocyte lysate (LAL) assays was purchased from Cape Cod Inc. (East Falmouth, MA). Hydrogen sulfate lactose medium (DHL) was purchased from Eiken Chem (Tokyo, Japan) and general anaerobic medium (GAM) and Blood liver medium (BL) were from Nissui Pharmaceutical Co. (Tokyo, Japan). MRS medium was purchased from BD (Radnor, PA).