Mouse monoclonal antibodies against CDH1 (#610181) and STAT3 (#610189) were obtained from BD Transduction Laboratories. Rabbit monoclonal anti-MMP-9 (#13667) and rabbit polyclonal anti-phosphor-STAT3 (#9131) antibodies were obtained from Cell Signaling Technology. Mouse anti-β-actin monoclonal antibody (#TA-09) and the rabbit monoclonal anti-CDH1 (#ZA-0565) and anti-MMP-9 (#ZA-0562) antibodies used in the IHC assay were purchased from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd. Purified AG490 powder (#T3434) was purchased from Sigma Aldrich.
Mouse anti β actin monoclonal antibody
Manufactured by Zhongshan Golden Bridge Biotechnology
Sourced in China
The Mouse anti-β-actin monoclonal antibody (#) is a laboratory tool used to detect and quantify the expression of the β-actin protein, a commonly used reference protein in various biological assays.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit monoclonal anti mmp9, by Cell Signaling Technology (1 mentions)
Rabbit anti np polyclonal antibody, by GeneTex (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Irak4, by Cell Signaling Technology (1 mentions)
First strand cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
3 protocols using mouse anti β actin monoclonal antibody
1
Antibody Detection in Mouse Samples
2
Antibody Generation and siRNA Knockdown
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Mouse anti-M1 monoclonal antibody and rabbit anti-NP polyclonal antibody were generated as previously described.40 (link) Rabbit anti-PB1 polyclonal antibody and rabbit anti-ATP6V1A polyclonal antibody were purchased from GeneTex (USA). Mouse anti-β-actin monoclonal antibody and secondary antibodies conjugated with horseradish peroxidase were from Zhongshan Goldenbridge Biotechnology (Beijing, China).
siRNA for ATP6V1A was synthesized by RiboBio (Guangzhou, China). The ATP6V1A siRNA sequences7 (link) were as follows: 5′-GAGCUUGAAUUUGAAGGUGUAdTdT-3′ (sense) and 5′-UACACCUUCAAAUUCAAGCUCdTdT-3′ (antisense).
siRNA for ATP6V1A was synthesized by RiboBio (Guangzhou, China). The ATP6V1A siRNA sequences7 (link) were as follows: 5′-GAGCUUGAAUUUGAAGGUGUAdTdT-3′ (sense) and 5′-UACACCUUCAAAUUCAAGCUCdTdT-3′ (antisense).
3
Molecular Mechanisms of LPS-Induced Inflammation
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Both 5-ethynyl-2′-deoxyuridine staining (EdU) and the Cell-Light TM EdU Kit were purchased from Rui Bo Guangzhou Biotechnology Limited Company (China). The elastic-van Gieson staining (EVG) staining reagent was purchased from Baso Zhuhai Biotechnology CO. LTD. (China). The streptavidin-peroxidase (SP) immunehistochemical assay kit, 3, 3′-diaminobenzidine (DAB) kit and mouse anti-β-actin monoclonal antibody were manufactured by Zhongshan Goldenbridge Biotechnology CO. LTD (China). Antibodies against proliferating cell nuclear antigen (PCNA), TLR4 and IL-1 receptor associated kinase (IRAK4) came from Cell Signaling Technology (USA). Antibodies against Inhibitors α of NFκB (IκBα), NFκBp65, Human antigen R (HuR) and EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) were purchased from Santa Cruz Biotechnology (USA). Lipopolysaccharides (LPS) (Escherichia coli 055:B5) was obtained from Sigma-Aldrich. Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM) and 0.25% trypsin were purchased from Gibco. Interleukin-6 (IL-6) Enzyme linked immunosorbent assay (ELISA) kit was purchased from Western Tang. TRIzol reagent, the first-strand cDNA synthesis kit and SYBR Green Master Mix were purchased from Invitrogen.
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