Human neurons

Manufactured by ScienCell

Sourced in United States, France

Human neurons are primary cells isolated from human brain tissue. They exhibit the characteristic morphology and biochemical properties of neuronal cells. These cells are suitable for in vitro studies of neuronal function and disease modeling.

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Lab products found in correlation

Human astrocyte, by ScienCell (2 mentions) Hcmec d3, by ScienCell (2 mentions) Irdye secondary antibody, by LI COR (1 mentions) Low fluorescence pvdf transfer membranes, by Thermo Fisher Scientific (1 mentions) Lipoteichoic acid lta, by Merck Group (1 mentions) Fluorescent secondary antibody, by Thermo Fisher Scientific (1 mentions) Neuronal medium, by ScienCell (1 mentions) Anti 3 tubulin, by Abcam (1 mentions) Human beta ngf elisa kit, by Merck Group (1 mentions) Hcpepic, by ScienCell (1 mentions) X vivo 15 medium, by Lonza (1 mentions) Phosstop phosphatase inhibitor cocktail, by Roche (1 mentions) Ripa buffer, by Thermo Fisher Scientific (1 mentions) Complete protease inhibitor, by Roche (1 mentions)

Close spelling variants of this product

Primary human cortical neurons (2 citations) Human primary neurons (2 citations) Human primary neuron cells (2 citations)

4 protocols using human neurons

Modeling Neuroinflammation and Mitochondrial Stress

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Primary human CNS cells or cell lines: human neurons (ScienCell,
Carlsbad, CA), human astrocytes (ScienCell), human brain endothelial cell line
(HCMEC/D3; provided by Pierre-Olivier Couraud (Weksler et al., 2005 (link))) and human microglia cell line (CHME5;
provided by Nazira El-Hage, Florida International University, Miami, FL) were
plated (10⁵ cells/ml) according to
manufacturer’s/provider’s instructions. These cells were treated
with respective culture medium (Control) or inflammatory stimuli (supernatant
from LPS- and CD3/CD28 beads-stimulated PBMCs, 50% volume/volume) or
mitochondrial-stress stimulus (rotenone, 100 nM). Cell-culture supernatants were
collected at 0 and 24 hours after respective treatments and stored at
−80°C until further use. Oligodendrocytes were differentiated from
human pluripotent stem cells as described (Douvaras and Fossati, 2015 (link)); differentiated cultures had 70% of
O4+oligodendrocytes.

Masvekar R., Wu T., Kosa P., Barbour C., Fossati V, & Bielekova B. (2018). Cerebrospinal Fluid Biomarkers Link Toxic Astrogliosis and Microglial Activation to Multiple Sclerosis Severity. Multiple sclerosis and related disorders, 28, 34-43.

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Investigating C5a-Mediated Neuronal Signaling

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Cell culture materials and reagents were from Corning (Manassas, VA, USA). Recombinant C5a and TrkA specific antagonist (AG-879) were from R&D Systems (Minneapolis, MN, USA). Anti-ß III tubulin and anti-Fibroblast Surface Protein (FSP) were from Abcam (Cambridge, UK). Anti-C5aR and anti-β-actin antibodies were respectively from Proteintech (Chicago, IL, USA) and BioLegend (San Diego, CA, USA). Fluorescent secondary antibodies were from Life Technologies (Grand Island, NY, USA), HRP-conjugated secondary antibody was from KPL (Gaithersburg, MD, USA) and IRDye secondary antibodies were from LI-COR (Lincoln, NE, USA). Low-fluorescence PVDF transfer membranes were from Thermo Scientific (Waltham, MA, USA). The C5aR antagonist (W54011), and chambers to evaluate the neurite outgrowth (AXIS) were from EMD Millipore (Darmstadt, Germany). The human beta NGF ELISA kit and lipoteichoic acid (LTA) were from Sigma-Aldrich (St. Louis, MO, USA), and chemicals were from Fisher Chemical (Nazareth, PA, USA). Human neurons, neuronal medium and neuronal growth supplement were from Sciencell (Carlsbad, CA, USA). All experimental protocols used for this study were in accordance with the guidelines according to the Institutional Animal Care and Use Policy and approved by the IRB Protocol Committee at the University of Illinois at Chicago.

Chmilewsky F., Ayaz W., Appiah J., About I, & Chung S.H. (2016). Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth. Scientific Reports, 6, 31799.

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Isolation and Profiling of CNS Cell Types

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Fresh peripheral blood mononuclear cells (PBMC) of two HD were obtained from Ficoll gradient-treated lymphocytapheresis samples. Monocytes, B-cells, CD4+ T cells, CD8+ T cells, natural killer cells, innate lymphoid cells, and myeloid dendritic cells were Fluorescence-activated cell sorted (FACS). Each purified cell subtype was cultured (1×10⁶ cells/ml) in serum-free X-VIVO 15 medium (Lonza, Walkersville, MD) with or without 10 μg/mL phorbol 12-myristate 13-acetate (PMA) and 1μM Ionomycin. Supernatants were collected after 48 hours and frozen until use.
Isolated primary human CNS cells or cell lines: human neurons (ScienCell, Carlsbad, CA), human astrocytes (ScienCell), human brain endothelial cell line (HCMEC/D3; provided by Pierre-Olivier Couraud, PhD, INSERM, France (13 (link))), human microglia cell line (CHME5; provided by Nazira El-Hage, PhD, Florida International University, USA), and human choroid plexus epithelial cells (hCPEpiC; ScienCell) were plated (10⁵ cells/ml; 10 ml/flask). Cells were treated with PBMC culture media (control) and an inflammatory mediators (supernatant from lipopolysaccharide- and CD3/CD28 beads-stimulated human PBMCs; 50% v/v). Oligodendrocytes were differentiated from the NIH approved human embryonic stem cell line RUES1 using published protocol (14 (link)). Cell-culture supernatants were collected after 24-hour incubation and frozen until use.

Barbour C., Kosa P., Komori M., Tanigawa M., Masvekar R., Wu T., Johnson K., Douvaras P., Fossati V., Herbst R., Wang Y., Tan K., Greenwood M, & Bielekova B. (2017). Molecular-based diagnosis of Multiple Sclerosis and its progressive stage. Annals of neurology, 82(5), 795-812.

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Preparation of Human Neurons and Astrocytes

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Human neurons (Cat# 1520) and astrocytes (Cat #1801) were obtained from ScienCell Research. Briefly, neurons were plated in 6-well poly-L-lysine-coated culture plates (Thermo 152035) according to manufactures' protocol. After 14 days in culture, neurons were washed briefly with ice-cold PBS and lysed in RIPA buffer (Thermo 89900) supplemented with Complete protease inhibitor and PhosSTOP phosphatase inhibitor cocktails (Roche Applied Sciences). For human astrocytes, cells were cultured following manufacture instructions and 0.5×10⁶ cells were sub-cultured in 6-well poly-L-lysine-coated culture plates for 24 hrs. astrocytes were then washed and lysed in the same manner as Human neurons. Lysates were solubilized at 4°C for 1 hr and cleared by centrifugation at 16,000 g for 5 min. Protein concentration was determined by the BCA protein assay kit (Pierce).

Zhou Y., Hayashi I., Wong J., Tugusheva K., Renger J.J, & Zerbinatti C. (2014). Intracellular Clusterin Interacts with Brain Isoforms of the Bridging Integrator 1 and with the Microtubule-Associated Protein Tau in Alzheimer's Disease. PLoS ONE, 9(7), e103187.

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