In all experiments, terbium chelate was the donor fluorophore with the acceptor fluorophores being Alexa Fluor 555 (Sigma-Aldrich) or Ni(NTA)2Cy3. Ni(NTA)2Cy3 was prepared as described previously (Kapanidis et al., 2001 (link)) using bis-reactive Cy3 purchased from GE Healthcare. DL-2-amino-5-phosphonopentanoic acid (DL-APV) and 5,7-dichlorokynurenic acid (DCKA), competitive antagonists of the NMDA receptor, were purchased from Abcam. Zinc and ifenprodil tartrate were purchased from Sigma-Aldrich.
Ifenprodil tartrate
Manufactured by Merck Group
Sourced in United States, France
Ifenprodil tartrate is a chemical compound used in laboratory research. It functions as an antagonist of the NMDA receptor, a type of glutamate receptor in the central nervous system. This compound is primarily utilized for its pharmacological properties in experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 555, by Merck Group (1 mentions)
Dl 2 amino 5 phosphonopentanoic acid dl apv, by Abcam (1 mentions)
Ro 25 6981, by Abcam (1 mentions)
Ketamine hydrochloride, by Jiangsu Hengrui Medicine (1 mentions)
Haloperidol, by Merck Group (1 mentions)
Pentazocine, by Merck Group (1 mentions)
Gbr 12909 dihydrochloride, by Merck Group (1 mentions)
3h pentazocine, by PerkinElmer (1 mentions)
3h dtg, by PerkinElmer (1 mentions)
Prazosin hydrochloride, by Merck Group (1 mentions)
Cyproheptadine hydrochloride, by Merck Group (1 mentions)
D amphetamine sulfate, by Merck Group (1 mentions)
5 protocols using ifenprodil tartrate
1
FRET Assay for NMDA Receptor
2
Preparation of Pharmacological Agents
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ifenprodil tartrate (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 5% dimethylsulfoxide and 9% Tween 80/saline before use.29 (link) Dihydrokainate (DHK; 6.25 nmol μl−1; Sigma-Aldrich) was dissolved in 0.01 M phosphate-buffered saline (pH 7.4).30 (link) TC-DAPK 6 (290 nM ) was dissolved in 2% dimethylsulfoxide and phosphate-buffered saline (pH 7.4).31 (link) Both Tat-GluN2BCT and Tat-sGluN2BCT were dissolved in saline.19 (link) Ketamine hydrochloride (10 mg kg−1) was obtained from Jiangsu Hengrui Medicine (Lianyungang, Jiangsu, China) and dissolved in saline. PEAQX (5 mg kg−1) and Ro 25–6981 (10 mg kg−1) were purchased from Abcam (Cambridge, UK) and dissolved in saline.
3
Compound Activity Testing in Oocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The compounds 1 , 2 , and 4 –7 were previously reported33 (link),34 (link),37 (link)–39 (link). Synthesis of compound 3 is described in the Supplementary Information (Supplementary Fig. 2 and Supplementary Methods). Ifenprodil (+)-tartrate was commercially available by Sigma Aldrich. Test compounds were used as 10 mM solutions in DMSO, which were diluted with agonist solution to obtain the desired concentration of compound and constant concentration of agonists. All test solutions were adjusted to DMSO concentrations of 0.1%. Compound activity was tested by two-electrode voltage clamp (TEVC) in Xenopus laevis oocytes at room temperature as described before34 (link). For measurements, oocytes were constantly superfused with Ba2+ Ringer (pH 7.4) containing (mmol L−1): 10 HEPES, 90 NaCl, 1 KCl, 1.5 BaCl2. Agonist solution containing 10 µM glycine and 10 µM (S)-glutamate was prepared from 100 mM stock solutions in Ba2+ Ringer for each measurement. Measurements were performed at a holding potential of −70 mV and recording pipettes (0.5–1.5 MΩ) were backfilled with 3 M KCl. Each compound concentration was tested at three different oocytes resulting in 12–24 oocytes for every dose-response curve depending on tested concentration levels
4
Radioligand Binding Assay Protocols
5
Behavioral Pharmacology in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Male C57BL/6J and DBA2 mice from Janvier (Le Genest St Isle, France) were used for the experiments. Animals arrived at 4 to 6 weeks of age and were 9 weeks-old at the beginning of the experiments. They were housed 4 per cage in a regulated environment (temperature: 22 ± 1°C, 12:12 h light/dark cycle, light on at 08:00 h, light/dark cycle is inversed for alcohol preference studies where) with food and water freely available.
The experiments were conducted in agreement with the institutional guidelines for use of animals and their care, in compliance with national and international laws and policies (council directives no. 87–848, October 19, 1987, Ministere de l’Agriculture et de la Foret, Service Veterinaire de la Sante et de la Protection Animale—Key-Obs’ agreement n° A 45-234-8, permission no. 45–15 J.C.B.). Mice were sacrificed by cervical dislocation. The experimental protocols and euthanasia have been approved by the Ethics Committee of Animal experimentation, registered number 27 at the French Ministry of Research. All efforts were made to minimize the number of animals used and their suffering.
All drugs (ifenprodil tartrate, cyproheptadine hydrochloride, d-amphetamine sulfate, prazosin hydrochloride), have been purchased at Sigma (France) and were injected intraperitoneally, doses being indicated as salts.
The experiments were conducted in agreement with the institutional guidelines for use of animals and their care, in compliance with national and international laws and policies (council directives no. 87–848, October 19, 1987, Ministere de l’Agriculture et de la Foret, Service Veterinaire de la Sante et de la Protection Animale—Key-Obs’ agreement n° A 45-234-8, permission no. 45–15 J.C.B.). Mice were sacrificed by cervical dislocation. The experimental protocols and euthanasia have been approved by the Ethics Committee of Animal experimentation, registered number 27 at the French Ministry of Research. All efforts were made to minimize the number of animals used and their suffering.
All drugs (ifenprodil tartrate, cyproheptadine hydrochloride, d-amphetamine sulfate, prazosin hydrochloride), have been purchased at Sigma (France) and were injected intraperitoneally, doses being indicated as salts.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!