Log In Sign up
The largest database of trusted experimental protocols

Py397 fak

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The PY397-FAK is a laboratory instrument designed for the detection and analysis of focal adhesion kinase (FAK) protein. It utilizes advanced spectroscopic techniques to measure the expression and activity of FAK, which is a key regulator of cell signaling pathways involved in cell adhesion, migration, and proliferation.

Automatically generated - may contain errors

Lab products found in correlation

P mlc2, by Cell Signaling Technology (4 mentions) Tenascin c, by Abcam (4 mentions) Total fak, by BD (4 mentions) Gapdh, by Cell Signaling Technology (4 mentions) Vimentin, by Merck Group (3 mentions) β actin, by Merck Group (3 mentions) Gapdh, by Merck Group (2 mentions) Fibronectin, by Abcam (2 mentions) Gli 1, by Thermo Fisher Scientific (2 mentions) Collagen 12a1, by Santa Cruz Biotechnology (2 mentions) Integrin β1, by Merck Group (2 mentions) Mabt409, by Thermo Fisher Scientific (2 mentions) P mypt1, by Merck Group (2 mentions) Collagen 3, by Abcam (2 mentions) Total stat3, by Cell Signaling Technology (2 mentions) Rock1, by Cell Signaling Technology (2 mentions) Rock2, by Cell Signaling Technology (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) P smad2 3, by Cell Signaling Technology (2 mentions) Vimentin, by Cell Signaling Technology (2 mentions)

Spelling variants of this product

Anti-pY397-FAK (11 citations) Phospho-FAK [pY397] (2 citations)

15 protocols using py397 fak

1

Oxidative Stress Response in HT-29 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HT-29 human colon carcinoma was obtained from ATCC and maintained in DMEM containing 10% FBS (Omega Scientific), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, and 100 units/ml penicillin and 100 μg/ml streptomycin. Diphenyliodonium (DPI), N-acetyl-L-cysteine (NAC), phorbol 12-myristate 13-acetate (PMA), horseradish peroxidase (HRP), luminol, H2O2, and 4-iodophenylboronic acid (4IPBA) were purchased from Sigma-Aldrich. Superoxide dismutase (SOD) was purchased from Abnova, and catalase (CAT) was purchased from MP Biomedicals.
FAK (Millipore), pY397 FAK (Invitrogen), pY402 Pyk2 (Cell Signaling), pY416 Src (Invitrogen), GAPDH (Millipore) antibody, and Alexa 488 phalloidin (Invitrogen) were obtained.
Kim J.S., Jeong K., Murphy J.M., Rodriguez Y.A, & Lim S.T. (2019). A Quantitative Method to Measure Low Levels of ROS in Nonphagocytic Cells by Using a Chemiluminescent Imaging System. Oxidative Medicine and Cellular Longevity, 2019, 1754593.
+ Open protocol
+ Expand
2

Immunohistochemistry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were as follows: a-SMA (Sigma-Aldrich C6198, 1:1000), GLi-1 (Thermo Scientific PA5-32206, 1:500), Tenascin C (Abcam AB108930, 1:500), Fibronectin (Abcam AB2413, 1:500), Collagen 12A1 (Santa Cruz Biotechnology E-15 sc-68449, 1:200), Sox2 (Abcam ab97959, 1:500), Vimentin (Cell Signaling 5741S, 1:200), FAP (Abcam AB53066, 1:500), Collagen III (Abcam AB7778, 1:500), YAP (Cell signaling 4912, 1:200), β1integrin (EMD Millipore MABT409, 1:500), pY397-FAK (Invitrogen 44625, 1:200), total FAK (BD Biosciences 610088, 1:1,000), ROCK1 (Cell Signaling C8F7, 1:1,000), ROCK2 (Cell Signaling D1B1, 1:1,000), p-MLC2 (Cell Signaling 3671, 1:200), p-MyPT1 (Millipore ABS45, 1:200), p-Stat3 (Cell Signaling 9145, 1:200), p-SMAD2/3 (Cell Signaling 8828, 1:200), total Stat3 (Cell Signaling 9132, 1:1,000), GAPDH (Cell Signaling 2118, 1:5,000), CD45 (BD Biosciences 550539, 1:200), CD68 (Thermo Scientific Ab-3, 1:200), Alexa Fluor-conjugated goat secondary anti–mouse IgG and anti–rabbit IgG antibodies (Invitrogen A11012, 1:1,000 and A11005, 1:1,000) and HRP-conjugated rabbit secondary antibody (GE health care life sciences NA934VS, 1:5,000).
Laklai H., Miroshnikova Y.A., Pickup M.W., Collisson E.A., Kim G.E., Barrett A.S., Hill R.C., Lakins J.N., Schlaepfer D.D., Mouw J.K., LeBleu V.S., Roy N., Novitskiy S.V., Johansen J.S., Poli V., Kalluri R., Iacobuzio-Donahue C.A., Wood L.D., Hebrok M., Hansen K., Moses H.L, & Weaver V.M. (2016). Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular-fibrosis and tumor progression. Nature medicine, 22(5), 497-505.
+ Open protocol
+ Expand
3

Antibody Validation for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were as follows (used at 1μg ml−1 for western blotting and chromatin immunoprecipitation (ChIP) and at indicated concentrations for immunofluorescence studies): tenascin C (Abcam ab108930; 1:1,000), pY397-FAK (Invitrogen 44625; 1:200), total FAK (BD Biosciences 610088), pMLC2 (Cell Signaling 3671; 1:200), total MLC2 (Abcam ab92721, clone EPR3741; 1:200), p-MyPT1 (Millipore ABS45; 1:200), hyaluronic acid binding protein (Calbiochem, 385911; 1:500), aggrecan (Abcam ab3778; 1:500), versican (Abcam ab19345; 1:500), collagen 1 (Abcam ab34710; 1:1,000), propidium iodide (AcrosOrganics 440300250; 1 μg ml−1), β-actin (Sigma-Aldrich a5441), HIF1α (Abcam ab-1, for immunofluorescence; 1:200; Abcam ab1, for ChIP; Novus 100-449, for western blotting), hypoxyprobe (Hypoxyprobe, HP1-100 Kit; per manual), CD31 (BD 550389; 1:500), laminin (Abcam ab11575; 1:500), RNA polymerase II (Millipore 05-623B), rabbit IgG isotype control (Cell Signaling 2729; 1:500), Alexa Fluor-conjugated goat secondary anti-mouse IgG and anti-rabbit IgG antibodies (Invitrogen A11012 and A11005; 1:500) and HRP-conjugated rabbit secondary antibody (GE Healthcare Life Sciences NA934VS; 1:5,000).
Miroshnikova Y.A., Mouw J.K., Barnes J.M., Pickup M.W., Lakins J.N., Kim Y., Lobo K., Persson A.I., Reis G.F., McKnight T.R., Holland E.C., Phillips J.J, & Weaver V.M. (2016). Tissue mechanics promote IDH1-dependent HIF1α–tenascin C feedback to regulate glioblastoma aggression. Nature cell biology, 18(12), 1336-1345.
+ Open protocol
+ Expand
4

Analyzing Focal Adhesion Kinase Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against pY397-FAK (Invitrogen), FAK (BD Biosciences), GAPDH (Cell Signalling), vimentin (Sigma), pY142-VAV2 (Full Moon Biosystems), pY172-VAV2 (Abcam), VAV2 (Full Moon Biosystems), GFP (Covance, Princeton, NJ), pY418-Src (Invitrogen) and FLAG (Sigma) were used for Western blotting, immunofluorescence and immunoprecipitation. HRP-conjugated secondary antibodies (Jackson ImmunoResearch) were used for Western blotting.
Havel L.S., Kline E.R., Salgueiro A.M, & Marcus A.I. (2014). Vimentin regulates lung cancer cell adhesion through a VAV2-Rac1 pathway to control focal adhesion kinase activity. Oncogene, 34(15), 1979-1990.
+ Open protocol
+ Expand
5

Analyzing Focal Adhesion Kinase Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against pY397-FAK (Invitrogen), FAK (BD Biosciences), GAPDH (Cell Signalling), vimentin (Sigma), pY142-VAV2 (Full Moon Biosystems), pY172-VAV2 (Abcam), VAV2 (Full Moon Biosystems), GFP (Covance, Princeton, NJ), pY418-Src (Invitrogen) and FLAG (Sigma) were used for Western blotting, immunofluorescence and immunoprecipitation. HRP-conjugated secondary antibodies (Jackson ImmunoResearch) were used for Western blotting.
Havel L.S., Kline E.R., Salgueiro A.M, & Marcus A.I. (2014). Vimentin regulates lung cancer cell adhesion through a VAV2-Rac1 pathway to control focal adhesion kinase activity. Oncogene, 34(15), 1979-1990.
+ Open protocol
+ Expand
6

Immunofluorescence Staining Antibodies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against ITGA6 (rat monoclonal GoH3), ITGB4 (mouse monoclonal 7/CD104), Cav1 (mouse monoclonal 2297/Caveolin1 and polyclonal rabbit), Rab11 (mouse monoclonal 47/Rab11), and EEA1 (mouse monoclonal 14/EEA1) were purchased from BD Transduction Laboratories; against CK14 (rabbit polyclonal) from Convance; against Plectin (polyclonal guinea pig) from Progen; against Arf6 (rabbit polyclonal) and Cavin1 (rabbit polyclonal) from AbCam; against pY397-FAK (polyclonal rabbit) from Invitrogen; and against α-tubulin (monoclonal mouse DM1A). EGF and all secondary antibodies used in immunofluorescence staining are coupled to Alexa Fluor 488, 594, or 647 dyes and were purchased from Molecular Probes.
Osmani N., Pontabry J., Comelles J., Fekonja N., Goetz J.G., Riveline D., Georges-Labouesse E, & Labouesse M. (2018). An Arf6- and caveolae-dependent pathway links hemidesmosome remodeling and mechanoresponse. Molecular Biology of the Cell, 29(4), 435-451.
+ Open protocol
+ Expand
7

Immunohistochemistry Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were as follows: a-SMA (Sigma-Aldrich C6198, 1:1000), GLi-1 (Thermo Scientific PA5-32206, 1:500), Tenascin C (Abcam AB108930, 1:500), Fibronectin (Abcam AB2413, 1:500), Collagen 12A1 (Santa Cruz Biotechnology E-15 sc-68449, 1:200), Sox2 (Abcam ab97959, 1:500), Vimentin (Cell Signaling 5741S, 1:200), FAP (Abcam AB53066, 1:500), Collagen III (Abcam AB7778, 1:500), YAP (Cell signaling 4912, 1:200), β1integrin (EMD Millipore MABT409, 1:500), pY397-FAK (Invitrogen 44625, 1:200), total FAK (BD Biosciences 610088, 1:1,000), ROCK1 (Cell Signaling C8F7, 1:1,000), ROCK2 (Cell Signaling D1B1, 1:1,000), p-MLC2 (Cell Signaling 3671, 1:200), p-MyPT1 (Millipore ABS45, 1:200), p-Stat3 (Cell Signaling 9145, 1:200), p-SMAD2/3 (Cell Signaling 8828, 1:200), total Stat3 (Cell Signaling 9132, 1:1,000), GAPDH (Cell Signaling 2118, 1:5,000), CD45 (BD Biosciences 550539, 1:200), CD68 (Thermo Scientific Ab-3, 1:200), Alexa Fluor-conjugated goat secondary anti–mouse IgG and anti–rabbit IgG antibodies (Invitrogen A11012, 1:1,000 and A11005, 1:1,000) and HRP-conjugated rabbit secondary antibody (GE health care life sciences NA934VS, 1:5,000).
Laklai H., Miroshnikova Y.A., Pickup M.W., Collisson E.A., Kim G.E., Barrett A.S., Hill R.C., Lakins J.N., Schlaepfer D.D., Mouw J.K., LeBleu V.S., Roy N., Novitskiy S.V., Johansen J.S., Poli V., Kalluri R., Iacobuzio-Donahue C.A., Wood L.D., Hebrok M., Hansen K., Moses H.L, & Weaver V.M. (2016). Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular-fibrosis and tumor progression. Nature medicine, 22(5), 497-505.
+ Open protocol
+ Expand
8

HaCaT Keratinocyte Culture and Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human skin keratinocytes, HaCaT cells, were obtained from the American Type Culture Collection (USA) and maintained Dulbecco’s modified essential medium containing 10% fetal bovine (Thermo Fisher Scientific, Waltham, MA, USA), 1 mM sodium pyruvate, 0.1 mM nonessential amino acids, 100 units/mL penicillin, and 100 μg/mL streptomycin. Diphenyliodonium (DPI), N-acetyl-L-cysteine (NAC), and z-DEVD-fmk were purchased from Sigma-Aldrich. FAK inhibitor (FAKi; PF-562,271) was obtained from MedKoo (Chapel Hill, NC, USA). Orange CM-H2TMRos and 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and MitoTracker Orange CM-H2TMRos were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies against FAK (Millipore, Burlington, MA, USA), pY397 FAK (Invitrogen), ERK, p-ERK, p-JNK, p-p38, p-IKKα, cleaved caspase-3, PARP (Cell Signaling, Danvers, MA, USA), and GAPDH (Millipore) antibody were obtained.
Kim J.S, & Lim S.T. (2022). LED Light-Induced ROS Differentially Regulates Focal Adhesion Kinase Activity in HaCaT Cell Viability. Current Issues in Molecular Biology, 44(3), 0.
+ Open protocol
+ Expand
9

Antibody Validation for Cellular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were as follows (used at 1μg ml−1 for western blotting and chromatin immunoprecipitation (ChIP) and at indicated concentrations for immunofluorescence studies): tenascin C (Abcam ab108930; 1:1,000), pY397-FAK (Invitrogen 44625; 1:200), total FAK (BD Biosciences 610088), pMLC2 (Cell Signaling 3671; 1:200), total MLC2 (Abcam ab92721, clone EPR3741; 1:200), p-MyPT1 (Millipore ABS45; 1:200), hyaluronic acid binding protein (Calbiochem, 385911; 1:500), aggrecan (Abcam ab3778; 1:500), versican (Abcam ab19345; 1:500), collagen 1 (Abcam ab34710; 1:1,000), propidium iodide (AcrosOrganics 440300250; 1 μg ml−1), β-actin (Sigma-Aldrich a5441), HIF1α (Abcam ab-1, for immunofluorescence; 1:200; Abcam ab1, for ChIP; Novus 100-449, for western blotting), hypoxyprobe (Hypoxyprobe, HP1-100 Kit; per manual), CD31 (BD 550389; 1:500), laminin (Abcam ab11575; 1:500), RNA polymerase II (Millipore 05-623B), rabbit IgG isotype control (Cell Signaling 2729; 1:500), Alexa Fluor-conjugated goat secondary anti-mouse IgG and anti-rabbit IgG antibodies (Invitrogen A11012 and A11005; 1:500) and HRP-conjugated rabbit secondary antibody (GE Healthcare Life Sciences NA934VS; 1:5,000).
Miroshnikova Y.A., Mouw J.K., Barnes J.M., Pickup M.W., Lakins J.N., Kim Y., Lobo K., Persson A.I., Reis G.F., McKnight T.R., Holland E.C., Phillips J.J, & Weaver V.M. (2016). Tissue mechanics promote IDH1-dependent HIF1α–tenascin C feedback to regulate glioblastoma aggression. Nature cell biology, 18(12), 1336-1345.
+ Open protocol
+ Expand
10

Quantifying Focal Adhesion Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies: pY410CAS (rabbit; Cell Signaling), pY165CAS (rabbit; Cell Signaling), pY118paxillin (rabbit; Sigma), paxillin (mouse, BD Transduction Laboratories), pY397FAK (rabbit; Invitrogen), pY861FAK (rabbit; Abgent), pY529Src (rabbit; Sigma), GFP (mouse; Invitrogen), actin (goat; Santa Cruz Biotechnology). Secondary antibodies (conjugated with horseradish peroxidase): goat anti-rabbit (Thermo Scientific), donkey anti-goat (Santa Cruz Biotechnology), rabbit anti-mouse (Abcam). AlexaFluor goat anti-mouse 594 (Molecular Probes) as a secondary antibody for immunofluorescence. For 3D STED imaging, Abberior STAR 635 (goat anti-rabbit) and Abberior STAR 580 (goat anti-mouse) were used as secondary antibodies.
Braniš J., Pataki C., Spörrer M., Gerum R.C., Mainka A., Cermak V., Goldmann W.H., Fabry B., Brabek J, & Rosel D. (2017). The role of focal adhesion anchoring domains of CAS in mechanotransduction. Scientific Reports, 7, 46233.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!