Human oesophageal cancer cell lines OE19, OE21 and OE33 were obtained from the European Collection of Cell Cultures (96071721, 96062201 and 96070808). KYSE450 cells were from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, ACC-387). OE21, OE19 and OE33 cells were grown in RPMI 1640 medium (Sigma, R8758). KYSE450 cells were maintained in 50: 50 RPMI: F12 HAMS medium (Sigma, N6658), all supplemented with 10 % foetal calf serum (Sigma, F7524) and 1 % Penicillin/Streptomycin (Gibco-BRL, 15070-063) and grown at 37 °C, 5 % CO2. Chloroquine (C6628) and 5-FU (F6627) were purchased from Sigma.
F12 hams medium
Manufactured by Merck Group
Sourced in Japan
F12 HAMS medium is a cell culture medium formulated for the cultivation of various mammalian cell lines. It is composed of a balanced salt solution, amino acids, vitamins, and other nutrients required for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
Foetal calf serum, by Merck Group (1 mentions)
Chloroquine c6628, by Merck Group (1 mentions)
5 fu f6627, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Merck Group (1 mentions)
Minimum essential medium (mem), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions)
3 protocols using f12 hams medium
1
Culturing Human Oesophageal Cancer Cell Lines
2
Culturing SH-SY5Y Neuroblastoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neuroblastoma cell lines SH- SY5Y (obtained from the ATCC) has been cultured in the mixture of Minimum Essential Medium (MEM, Sigma) and F − 12 Ham’s medium (Sigma) in 1:1 ratio with the addition of 1% of glutamine, penicillin-streptomycin solution, NEAA – nonesencial aminoacids and 10% fetal bovine serum (Sigma). The cell culture was cultivated in a CO2 incubator at 37°С and 5% CO2. Cells were subcultured every 5–7 days. The medium was changed every 3 days.
3
Rat Gastric Carcinoma Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An MNNG-induced mutant of the rat murine RGM-1 gastric carcinoma mucosal cell line (RGK-1) was kindly provided by Dr. Hirofumi Matsui (Faculty of Medicine, University of Tsukuba, Ibaraki, Japan). RGK-1 cells were grown in a 1:1 mixture of Dulbecco’s modified Eagle’s medium (DMEM) and F12 Ham’s medium (Sigma-Aldrich Japan, Tokyo, Japan) supplemented with 10 vol% fetal calf serum (FCS; Hyclone Laboratories, Inc., Logan, UT, USA) and 1 vol% Antibiotic-Antimycotic (Life Technologies, Tokyo, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!