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4 protocols using 3 carene

1

Characterization of B. thermosphacta and P. fluorescens

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The Gram-positive bacterium B. thermosphacta ACCC 03,870 was purchased from the Agricultural Culture Collection of China, and the Gram-negative bacterium P. fluorescens ATCC 13,525 was obtained from the Guangdong Culture Collection Center. The strains were stored in sterile slants of nutrient agar (NA) at 4 °C. The (+)-3-Carene was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The SDH, MDH, PK, and ATP assay kits were purchased from Nanjing JianCheng Bioengineering Institute (Nanjing, China). Rhodamine 123 was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals were of analytical grade unless otherwise mentioned.
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2

Antifungal Compounds Screening Protocol

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Miconazole, diflucan (fluconazole), and 5-fluorocytosine were purchased from Combi-Blocks Inc. (San Diego, CA, USA). Itraconazole and voriconazole were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). 2-(4-Thiazolyl) benzimidazole (thiabendazole, TBZ) was obtained from San-ai Oil Co., Ltd. (Tokyo, Japan). Amphotericin B and terpinen-4-ol were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan), and α-pinene, camphene, 3-carene, limonene, γ-terpinene, 1,8-cineole, and p-cymene were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
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3

Characterization of P. lundensis Strain

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P. lundensis ATCC 49,968 was purchased from the BeNa Culture Collection (Beijing, China). Before all experiments, the strain was revitalized at 28 °C in nutrient agar.
The (+)-3-Carene (≥90.0%) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Rhodamine 123 was purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Coomassie Brilliant Blue G250 was purchased from Aladdin Co., Ltd. (Shanghai, China). The ATPase, adenosine triphosphate (ATP), bicinchoninic acid (BCA), and alkaline phosphatase (AKP) assay kits were purchased from Nanjing JianCheng Bioengineering Institute (Nanjing, China). All other chemicals were analytical grade.
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4

Sausage Aroma Compound Analysis

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Three sausage types with different fat content (regular (32%) , half-added (16%) , no added fat) were prepared.
Aromatic compounds, including β-pinene, 3-carene, D-limonene, octanal, nonanal, caryophyllene, methyl eugenol, hexanoic acid, guaiacol, 4-ethyl-2-methoxyphenol, 1, 2, 4-trimethylbenzene m-cresol, and eugenol, and lecithin (from eggs) were sourced from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan) . Triolein and cholesterol (purity> 99%) were purchased from Merck KGaA (Darmstadt, Germany) .
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