Head space gas chromatography ion mobility spectrometry (FlavourSpec 1H1-00206, G.A.S. Gesellschaft für analytische Sensorsysteme mbH) was described by Yijun et al.19 With some modification about the parameters. The green coffee samples (1.0 g each) was transferred to a 20 mL head vial and heated in an incubator at an incubator at an oscillation rate of 500 rpm, 60 °C for 20 min. Then, the samples were injected into a quartz capillary column (MXT-WAX, 30 m × 1 μm × 0.53 mm) by nitrogen at a programmed flow as follows: 2 mL min−1 for 2 min, 10 mL min−1 for 8 min, 100 mL min−1 for 30 min, and the syringe temperature was 85 °C and injection volume was 200 μL by a headspace automatic sampler (CTC CombiPAL, CTC Analytics AG). The compound were driven to the ionization chamber to be ionized in a positive ion mode by a 3H ionization source, and each spectrum was scanned 12 times on average. Then, the positive ions generated were separated in the drift tube for a second time, the length of the drift tube was 98 mm, the drift tube was operated at a constant temperature of 45 °C, and a voltage of 500 v cm−1. The drift gas (nitrogen) was set to 150 mL min−1. All analyses were performed in triplicate.
Ctc combipal
Manufactured by CTC Analytics
Sourced in Switzerland
The CTC CombiPAL is a versatile autosampler that can be integrated with a variety of analytical instruments. It is designed to handle a wide range of sample types and volumes, providing automated sample handling and preparation capabilities. The CombiPAL is capable of performing tasks such as liquid, headspace, and solid-phase microextraction (SPME) sampling.
Automatically generated - may contain errors
Lab products found in correlation
Trace gc ultra, by Thermo Fisher Scientific (5 mentions)
Stableflex, by Merck Group (4 mentions)
Tsq 7000, by Thermo Fisher Scientific (3 mentions)
Dvb car pdms spme fiber, by Merck Group (2 mentions)
Spme fibre, by Merck Group (1 mentions)
J w scientific, by Agilent Technologies (1 mentions)
Glc 462, by Nu-Chek Prep (1 mentions)
Gcms qp2010 ultra, by Shimadzu (1 mentions)
14 protocols using ctc combipal
1
Headspace GC-IMS for Green Coffee Aroma Analysis
2
Static Headspace SPME-GC/MS Analysis
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Samples were loaded into vials (20 mL headspace) sealed with a silicon/PTFE septum prior to SPME-GC/MS analysis. Sample preparation using the static headspace solid-phase microextraction (sHS-SPME) technique was carried out with a CTC Combi PAL (CTC Analytics AG, Zwingen, Switzerland) automatic multipurpose sampler using a 65 μM StableFlex polydimethyl siloxane/carboxene/divinyl benzene (CAR/PDMS/DVB) SPME fiber (Supelco, Bellefonte, PA, USA). After an incubation period of 5 min at 100 °C, extraction was performed by exposing the fiber to the headspace of a 20 mL vial containing the sample for 10 min at 100 °C. The fiber was then immediately transferred to the injector port of the GC/MS and desorbed for 1 min at 250 °C, in split mode, and the split ratio was 1:90. The SPME fiber was cleaned and conditioned in a Fiber Bakeout Station in a pure nitrogen atmosphere at 250 °C for 15 min.
3
Volatile Metabolite Profiling of Plants
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Plant material (pooled samples of 0.5–1 g fresh mass of either several fully developed, i.e., mature leaves, or young leaves and shoot tips or cotyledons as indicated) was put into vials (20 mL headspace) sealed with a silicon/polytetrafluoroethylene septum prior to the SPME gas chromatography/mass spectrometry (SPME-GC/MS) analysis. Sample preparation using the static headspace solid phase microextraction (sHS-SPME) technique was carried out with a CTC Combi PAL (CTC Analytics AG, Switzerland) automatic multipurpose sampler using a 65 μM StableFlex polydimethyl siloxane/carboxene/divinyl benzene (CAR/PDMS/DVB) SPME fiber (Supelco, United States). After an incubation period of 5 min at 100°C, extraction was performed by exposing the fiber to the headspace of a 20 mL vial containing the sample for 10 min at 100°C. The fiber was then immediately transferred to the injector port of the GC/MS, and desorbed for 1 min at 250°C. Injections were made in splitless mode. The SPME fiber was cleaned and conditioned in a Fiber Bakeout Station in pure nitrogen atmosphere at 250°C for 15 min.
4
Electron Ionization Mass Spectrometry for Chemical Identification
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The electron ionization (EI) mass spectrum was recorded using a Finnigan TSQ 7000 triple stage quadrupole mass spectrometer coupled to a gas chromatograph (Trace GC Ultra, Thermo Electron, Dreieich, Germany). Sample introduction was carried out using a CTC CombiPAL (CTC Analytics, Zwingen, Switzerland) autosampler. The emission current was 200 μA and the scan time was 1 s spanning a scan range of m/z 29–600. The ion source temperature was maintained at 175°C. Samples were introduced via gas chromatography (GC) with splitless injection using a fused silica capillary DB-1 column (30 m × 0.25 mm, film thickness 0.25 μm). The temperature program consisted of an initial temperature of 80°C, held for 1 min, followed by a ramp to 280°C at 15°C/min. The final temperature was held for 21 min and the injector temperature was 220°C. The transfer line temperature was maintained at 280°C and the carrier gas was helium in constant flow mode at a flow rate of 1.2 mL/min. Approximately 2 mg was dissolved in 1.5 mL chloroform. For analysis, 1 μL sample solution was injected into the GC–MS system. Retention indices (RI) are given as Kovats indices calculated from measurement of an n-alkane mixture analyzed with the above-mentioned temperature program.
5
Electron Ionization Mass Spectrometry for Chemical Identification
6
Characterization of Volatile Compounds in Fermented Sausages
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After the isolation of sulphur and nitrogen compounds from the fermented sausages, the SAFE extract was analysed by GC and multidimensional GC (MDGC) in combination with different detection systems (mass selective detector, flame photometric and nitrogen-phosphorous detectors). Different GC equipments were used and all of them were equipped with an autosampler CTC Combi Pal (CTC Analytics AG, Zwingen, Switzerland).
7
Volatile Compounds Analysis of Lemongrass Oil and TLC Spots
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The lemongrass EO sample and the white spots from the developed TLC-based bioautography plates were analysed with HS-SPME. In the case of lemongrass EO the liquid EO was analysed, while in the case of the TLC spot the cut-out silica plate was the analysed sample. The samples were put into vials (20 mL) and sealed with a silicon/PTFE septum prior to HS-SPME/GC-MS analysis. Sample preparation using the static headspace solid-phase microextraction (sHS-SPME) technique was carried out with a CTC Combi PAL (CTC Analytics AG, Zwingen, Switzerland) automatic multipurpose sampler using a 65 μM StableFlex carboxen/polydimethylsiloxane/divinylbenzene (CAR/PDMS/DVB) SPME fibre (Supelco, Bellefonte, PA, USA). After an incubation period of 5 min at 100 °C, extraction was performed by exposing the fibre to the headspace of a 20 mL vial containing the sample for 10 min at 100 °C. The fibre was then immediately transferred to the injector port of the GC/MS and desorbed for 1 min at 250 °C. Injections were made in splitless mode. The SPME fibre was cleaned and conditioned in a fibre bakeout station in a pure nitrogen atmosphere at 250 °C for 15 min.
8
GC-MS Analysis of Organic Compounds
9
GC-MS Analysis of Organic Compounds
10
Essential Oil Profiling via sHS-SPME GC/MS
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Pooled intact and fresh third and fourth leaf pairs (0.5–1 g fresh mass) were used for the essential oil analyses. Samples were put into vials (20 ml headspace) sealed with a silicon/polytetrafluoroethylene septum prior to the static headspace solid-phase microextraction (sHS-SPME). A CTC Combi PAL (CTC Analytics AG, Switzerland) automatic multipurpose sampler was used for the sample preparation by applying the static headspace solid-phase microextraction (sHS-SPME) technique using a 65 mM StableFlex carboxene/polydimethylsiloxane/divinylbenzene (CAR/PDMS/DVB) SPME fiber (Supelco, US). After 5 min incubation at 100°C, the fiber was exposed to the headspace of the 20-ml vial containing the sample for 10 min at 100°C in order to obtain extraction. After this, the fiber was immediately transferred to the injector port of the gas chromatograph/mass spectrometer (GC/MS) and desorbed for 1 min at 250°C. The splitless mode was used for injections. In all cases, the SPME fiber was cleaned and conditioned in a Fiber Bakeout Station in a pure nitrogen atmosphere at 250°C for 15 min. SPME gas chromatography/mass spectrometry (SPME-GC/MS) analysis was performed after this.
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