Anti sumo2 3 ab81371

Primary antibodies specific for HBV proteins included anti-HBV core protein mouse monoclonal antibody (MAb) 8C9-11 (72 (link)), anti-HBV core protein mouse MAb sc-23945 (Santa Cruz), both recognizing linear epitopes within the HBV core protein, and HBV capsid/capsid-intermediate-specific rabbit polyclonal antibody (PAb) B0586 (Dako). Primary antibodies used for epitope tags and cellular targets included anti-HA rat MAb 3F10 (Core Facility for Monoclonal Antibodies, Helmholtz Zentrum München), mouse MAb against the His₆ tag (Clontech), anti-PML protein mouse MAb sc-966 (Santa Cruz), polyclonal rabbit anti-PML protein PAb NB100-59787 (Novus Biologicals), polyclonal rabbit anti-PML protein ab72137 (Abcam), monoclonal mouse anti-SUMO2/3 ab81371 (Abcam), and anti-beta-actin mouse MAb AC-15 (Sigma-Aldrich). Secondary antibodies conjugated to Alexa Fluor 488 were purchased from Thermo Scientific, and secondary antibodies coupled to horseradish peroxidase or Alexa Fluor 647 were anti-rabbit IgG, anti-mouse IgG, anti-mouse light chain IgG, and anti-rat IgG (all from Dianova).

Etoposide and 2′,3′,4′-trihydroxy-flavone (2-D08) were purchased from Sigma-Aldrich (Dorset, UK); PR-619 was obtained from Tocris Biosciences (Bristol UK); {(1R,2R,3S,4R)-2,3-dihydroxy-4-[(2-{3-[(trifluoromethyl)sulfanyl]phenyl}pyrazolo[1,5-a]pyrimidin-7-yl)amino]cyclopentyl}methyl sulfamate (MLN7243) was obtained from Active Biochem Ltd. (Hong Kong); and {(1R,2S,4R)-4-[(5-{[1-(3-bromobenzyl)-1H-pyrazol-3-yl]carbonyl}-4-pyrimidinyl)amino]-2-hydroxycyclopentyl}methyl sulfamate (ML-792) was obtained from Bioquote (York, UK). Rabbit anti-TOP2A (4566) and anti-TOP2B (4555) antibodies were raised in-house to the C-terminal domains of the respective proteins (Atwal et al., 2019 (link)). Anti-ubiquitin FK2 (anti-mono and poly-ubiquitinated conjugates, K²⁹, K⁴⁸, or K⁶³ linked) was obtained from Enzo (Exeter, UK), and anti-SUMO2/3 (Ab81371) was from Abcam (Cambridge UK).