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Ph strips

Manufactured by Thermo Fisher Scientific
Sourced in Germany

pH strips are a simple and affordable tool used to measure the pH level of a solution. They provide a quick and easy way to determine the acidity or basicity of a substance. The strips are dipped into the solution, and the resulting color change is compared to a provided pH scale to determine the exact pH value.

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4 protocols using ph strips

1

Milk-Based Cell Culture Protocol

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Milk products including whole milk, 2% skim, and fat-free half-pint (236 ml) cartons were obtained from a local retail food market (Prairie Farms, Springfield, IL). A total of 11 boxes of each kind of milk, with expiration dates on and up to 10 days prior to the day of experiment, were used. Distilled water (1 gallon bottle) was obtained from Walmart (Bentonville, AR). Agarose (30 g bottle) was obtained from Biomedicals (Santa Ana, CA). Fetal bovine serum (FBS; 100 ml bottle) was obtained from HyClone (Logan, UT). Petri dishes (60 mm diameter), glass beaker, pH strips, hotplate stirrer and magnetic stir bar pipette, and pipette tips were obtained from Fisher Scientific (Pittsburgh, PA). Kitchen scale was obtained from Taylor (Oak Brooks, IL). The M150 biological microscope was obtained from Am Scope (Irvine, CA). The computer was obtained from Lenovo (Quarry Bay, Hong Kong), Image J software was obtained from NIH (Bethesda, Maryland), and Excel software was obtained from Microsoft (Redmond, Washington).
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2

Platelet pH Measurement Protocol

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The pH in the stored platelets was determined by the Fisher Scientific pH strips.10 (link)
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3

Lactic acid and pH analysis

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Scaffolds were incubated in MRS media and the materials in suspension were collected at the corresponding time points (0, 24, 48, 72, 96, 120, 144, and 168 hr, and 2, 3, and 4 wk) were evaluated for lactic acid levels and pH. Briefly, 1 mL of suspension was centrifuged at 2500×g for 5 min to separate any bacteria from the media supernatant. The solution was then subjected to 10-fold serial dilutions. Concentrations of L- and D-lactic acid were determined with a D-Lactic acid/L-lactic acid detection kit (R-biopharma; Darmstadt, Germany) and the corresponding pH of supernatants at each time point was estimated using pH strips (Fisher Scientific) with a pH range of 1 to 6 and an accuracy of 0.5 pH units.
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4

Characterization of Irradiated Mouth Rinses

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The color, sedimentation, precipitation of control, and irradiated mouth rinses were recorded immediately after E-beam irradiation. The pH of mouth rinses was recorded using pH strips (Fisher Scientific) of range 2-10.
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