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Anti cd25 clone 7d4

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Anti-CD25 (clone 7D4) is a laboratory reagent. It is an antibody that specifically binds to the CD25 antigen.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd8 clone 53 6, by BD (2 mentions) Anti cd3 cd28 antibody coated beads, by Thermo Fisher Scientific (2 mentions) Miltenyi mouse pan t cell kits, by BD (2 mentions) Automacs, by Miltenyi Biotec (2 mentions)

Spelling variants of this product

CD25 (7D4, (5 citations) PE anti-CD25 (clone no. 7D4, (2 citations) Anti-CD25-biotinylated (clone 7D4, (2 citations) Anti-CD25-FITC (clone 7D4, (2 citations) FITC-conjugated anti-CD25 (clone 7d4), (2 citations)

2 protocols using anti cd25 clone 7d4

1

Isolation and Activation of Mouse CD4+CD25- T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse CD4+CD25- T cells were isolated from mouse spleens using Miltenyi mouse pan T-cell kits supplemented with biotinylated anti-CD8 (clone 53–6.7; BD Pharmingen, San Diego, CA), anti-CD25 (clone 7D4; BD Pharmingen), and an AutoMACS (Miltenyi Biotech, Auburn, CA). The isolated CD4+CD25− T cells were activated for 3 days in the presence of anti-CD3/CD28 antibody-coated beads (bead:cell = 1:1; Invitrogen, Carlsbad, CA) and Xcyte media with 10 U/ml of hIL-2.13 (link)
Choi J., Cooper M.L., Staser K., Ashami K., Vij K.R., Wang B., Marsala L., Niswonger J., Ritchey J., Alahmari B., Achilefu S., Tsunoda I., Schroeder M.A, & DiPersio J.F. (2018). Baricitinib-induced blockade of interferon gamma receptor and interleukin-6 receptor for the prevention and treatment of graft-versus-host disease. Leukemia, 32(11), 2483-2494.
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2

Isolation and Activation of Mouse CD4+CD25- T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse CD4+CD25- T cells were isolated from mouse spleens using Miltenyi mouse pan T-cell kits supplemented with biotinylated anti-CD8 (clone 53–6.7; BD Pharmingen, San Diego, CA), anti-CD25 (clone 7D4; BD Pharmingen), and an AutoMACS (Miltenyi Biotech, Auburn, CA). The isolated CD4+CD25− T cells were activated for 3 days in the presence of anti-CD3/CD28 antibody-coated beads (bead:cell = 1:1; Invitrogen, Carlsbad, CA) and Xcyte media with 10 U/ml of hIL-2.13 (link)
Choi J., Cooper M.L., Staser K., Ashami K., Vij K.R., Wang B., Marsala L., Niswonger J., Ritchey J., Alahmari B., Achilefu S., Tsunoda I., Schroeder M.A, & DiPersio J.F. (2018). Baricitinib-induced blockade of interferon gamma receptor and interleukin-6 receptor for the prevention and treatment of graft-versus-host disease. Leukemia, 32(11), 2483-2494.
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