Antibodies: anti-HA tag (clone 3F10) (Roche Applied Science), anti-β-actin and anti-alpha-tubulin (Sigma-Aldrich), anti-Smac (Upstate Cell Signaling Solutions, Lake Placid, NY), anti-Hsp60 (Enzo Life Sciences, Plymouth Meeting, PA), anti-VDAC1 (Calbiochem, Darmstadt, Germany), anti-GAPDH, anti-PDK4 and anti-Vinculin (Santa Cruz), anti-PGC-1α (a gift from Dr. Daniel P. Kelly, Sanford-Burnham Medical Research Institute, FL), anti-pAMPK, anti-pCREB and anti-CREB (Cell Signaling Technologies, Boston, MA), anti-CHCHD4 (Protein-Tech Group, IL), anti-Tim23 (BD Biosciences, San Diego, CA). The peroxidase-conjugated goat anti-rat, goat anti-rabbit, goat anti-mouse and horse anti-goat antibodies were from Vector Laboratories (Burlingame, CA). Rabbit polyclonal antibodies specific for human CHTM1 were generated in our laboratory through ProSci Inc. (Poway, CA) using full-length recombinant human CHTM1 protein purified from Escherichia coli. For cell transfections, Mirus (Madison, WI) and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) were used. Restriction endonucleases were from New England BioLabs (Ipswich, MA). PKA inhibitor-H89, p38 inhibitor-SB203580, PI3K inhibitor-LY294002 and PKC inhibitor-GO6983 were from Sigma-Aldrich (St. Louis, MO). Other chemical reagents were from Thermo Fisher Scientific and Sigma-Aldrich.
Anti ha tag clone 3f10
Manufactured by Roche
The Anti-HA tag (clone 3F10) is a monoclonal antibody that specifically binds to the HA (Hemagglutinin) tag, a commonly used epitope tag for protein expression and detection. The antibody can be used in various immunological techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to identify and track proteins carrying the HA tag.
Automatically generated - may contain errors
Lab products found in correlation
Anti chchd4, by Proteintech (3 mentions)
Anti β actin and anti alpha tubulin, by Merck Group (3 mentions)
P38 inhibitor sb203580, by Merck Group (3 mentions)
Restriction endonuclease, by New England Biolabs (3 mentions)
Anti tim23, by BD (3 mentions)
Anti pgc 1α, by Santa Cruz Biotechnology (2 mentions)
Pka inhibitor h89, by Merck Group (2 mentions)
Anti hsp60, by Enzo Life Sciences (2 mentions)
Pkc inhibitor go6983, by Merck Group (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Pi3k inhibitor ly294002, by Merck Group (2 mentions)
Anti gfp b 2, by Santa Cruz Biotechnology (1 mentions)
Anti hdac1, by Proteintech (1 mentions)
Anti gr, by Proteintech (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Anti flag dykddddk tag, by Cell Signaling Technology (1 mentions)
Anti myc clone 9e10, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Lipojet, by SignaGen (1 mentions)
Cleaved parp, by Santa Cruz Biotechnology (1 mentions)
4 protocols using anti ha tag clone 3f10
1
Antibody Reagents for Western Blot Analysis
2
Antibody Reagents for Western Blot Analysis
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Antibodies: anti-HA tag (clone 3F10) (Roche Applied Science), anti-β-actin and anti-alpha-tubulin (Sigma-Aldrich), anti-Smac (Upstate Cell Signaling Solutions, Lake Placid, NY), anti-Hsp60 (Enzo Life Sciences, Plymouth Meeting, PA), anti-VDAC1 (Calbiochem, Darmstadt, Germany), anti-GAPDH, anti-PDK4 and anti-Vinculin (Santa Cruz), anti-PGC-1α (a gift from Dr. Daniel P. Kelly, Sanford-Burnham Medical Research Institute, FL), anti-pAMPK, anti-pCREB and anti-CREB (Cell Signaling Technologies, Boston, MA), anti-CHCHD4 (Protein-Tech Group, IL), anti-Tim23 (BD Biosciences, San Diego, CA). The peroxidase-conjugated goat anti-rat, goat anti-rabbit, goat anti-mouse and horse anti-goat antibodies were from Vector Laboratories (Burlingame, CA). Rabbit polyclonal antibodies specific for human CHTM1 were generated in our laboratory through ProSci Inc. (Poway, CA) using full-length recombinant human CHTM1 protein purified from Escherichia coli. For cell transfections, Mirus (Madison, WI) and Lipofectamine 2000 (Invitrogen, Carlsbad, CA) were used. Restriction endonucleases were from New England BioLabs (Ipswich, MA). PKA inhibitor-H89, p38 inhibitor-SB203580, PI3K inhibitor-LY294002 and PKC inhibitor-GO6983 were from Sigma-Aldrich (St. Louis, MO). Other chemical reagents were from Thermo Fisher Scientific and Sigma-Aldrich.
3
Antibody Detection and Quantification
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The following antibodies were used for Western blots (WB), immunofluorescence (IF), and Filter trap (FT): anti‐DYKDDDDK(FLAG) Tag (Cell Signaling #2368S) WB 1/1,000, anti‐myc clone 9E10 (Santa Cruz) WB 1/1,000, IF 1/100, anti‐HA Tag clone 3F10 (Roche) WB 1/1,000, IF 1/100, anti‐GFP(B‐2) (Santa Cruz) WB 1/500, FT 1/500, anti‐beta‐actin (Sigma‐Aldrich) WB 1/1,000, FT 1/1,000, anti‐EXOSC10 (ATLAS ANTIBODIES) WB 1/500, IF 1/2,000, anti‐DIS3 (Proteintech) WB1/2,000, anti‐DIS3L (Santa Cruz) WB 1/500, anti‐GAPDH (Proteintech) WB 1/20,000, anti‐HDAC1 (Proteintech) 1/1,000, anti‐PR (Proteintech) WB 1/500, FT 1/500, anti‐GR (Proteintech) WB 1/1,000, FT 1/1,000.
4
Characterization of Protein Expression and Apoptosis
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We used the following antibodies: anti-HA tag (clone 3F10) (Roche Applied Science), anti-β-actin and anti-alpha-tubulin (Sigma-Aldrich), anti-GAPDH and anti-Vinculin (Santa Cruz), cleaved PARP, pro-caspase 3 and pro-caspase 8, phospho-H2AX, AIF1, p38, phospho-p38, phospho- Hsp27, phospho-MAPKAP2 (Cell Signaling Technologies, Boston, MA), anti-CHCHD4 (Protein Tech, IL), anti-Tim23 (BD Biosciences, San Diego, CA), cytochrome c (Thermofisher Scientific, MA), Smac (Upstate cell signaling, NY). The peroxidase-conjugated anti-rat, anti-rabbit, anti-mouse and anti-goat antibodies were from Vector Laboratories (Burlingame, CA). Rabbit polyclonal antibodies specific for human CHTM1 and CHCM1/Mic25 were produced via ProSci Inc. (Poway, CA) against full-length recombinant protein. For cell transfections, Polyjet and Lipojet (Signagen Laboratories, Rockville, MD) were used. Expression construct sub-cloning was performed using restriction endonucleases from New England BioLabs (Ipswich, MA). p38 inhibitor-SB203580 was from Sigma-Aldrich (St. Louis, MO) and pan-caspase inhibitor- Z-VAD-FMK was from BD Biosciences (San Jose, CA, USA). Other chemical reagents were obtained from Sigma-Aldrich and Thermo Fisher Scientific.
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