Rbbp5

Manufactured by Cell Signaling Technology

Sourced in United States

RBBP5 is a laboratory reagent produced by Cell Signaling Technology. It is a recombinant protein that can be used in various biochemical and cell-based assays. The core function of RBBP5 is to serve as a tool for researchers in the study of cellular processes and signaling pathways.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Glutamine, by Merck Group (1 mentions) Vegf165, by Thermo Fisher Scientific (1 mentions) Matrigel for tube assay, by Corning (1 mentions) H3k27me3, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) α mem, by Thermo Fisher Scientific (1 mentions) 2 7 dichlorofluorescin diacetate dcfda, by Merck Group (1 mentions) Histone h3, by Merck Group (1 mentions) Rna polymerase 2, by Abcam (1 mentions) Wdr82, by Abcam (1 mentions) H3k4me3, by Abcam (1 mentions) Ptbp1, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) H3k27ac, by Abcam (1 mentions) Protease inhibitor cocktail, by Santa Cruz Biotechnology (1 mentions) Glial fibrillary acidic protein (gfap), by Thermo Fisher Scientific (1 mentions) Laemmli buffer, by Bio-Rad (1 mentions) Nanog, by Cell Signaling Technology (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

4 protocols using rbbp5

Investigating Lymphangiogenesis Signaling Pathways

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Cell culture medium (α-MEM and DMEM) and FBS were purchased from GIBCO Life Technologies. Penicillin and streptomycin were obtained from HyClone. Glutamine was obtained from Sigma. Matrigel for tube assay and cell invasion assay was obtained from Corning. Transwell inserts (PIXP01250) were purchased from Merck Millipore. Anti-PROX1 (BA2390) for Western blot and anti-PROX1 (ab199359) for immunohistochemistry and immunofluorescence were purchased from Boster and Abcam, respectively. Anti-LANA (ab4103) antibody for immunohistochemistry and immunofluorescence was purchased from Abcam. Antibodies against H3K4me3 (07–473) and H3K27me3 (07–449) were from Merck Millipore, and methyltransferases CFP1 (CXXC1,40672), EZH2 (5246), MLL2 (63735), WDR5 (13105) and RBBP5 (13171) were from Cell Signaling Technology, Inc. Antibody against UTX (ab36938) was from Abcam. Antibody against MLL1 (61295) was purchased from Active Motif. Human IL-6 (hIL-6) (14-8069-62) was from eBioscience, and VEGF-165 (100–20) was obtained from PeproTech, Inc. Antibody against VEGF-A (YT5108) was from ImmunoWay. PDPN (11629-1-AP), CD31 (11265-1-AP), VEGFR2 (26415-1-AP), VCAM1 (11444-1-AP) were from ProteinTech. LYVE-1 (sc-19316) and VEGFR3 (a5605) were obtained from Santa Cruz and Abclonal, respectively. Neutralizing antibody VEGF-A (11066-R010) were purchased from Sino Biological.

Ding Y., Chen W., Lu Z., Wang Y, & Yuan Y. (2021). Kaposi’s sarcoma-associated herpesvirus promotes mesenchymal-to-endothelial transition by resolving the bivalent chromatin of PROX1 gene. PLoS Pathogens, 17(9), e1009847.

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Antibody Panel for Protein Analysis

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The following antibodies were used in this study: FLAG (F1804; 1:2,000 for Western blot [WB]) and β-actin (A1978; 1:10,000 for WB) from Sigma-Aldrich; histone H3 (ab1791; 1:10,000 for WB), RNA polymerase II (phospho S5; ab5408; 1:200 for ChIP), H3K4me3 (ab8580; 1:200 for ChIP and Cut&Tag), and H3K27ac (ab6002; 1:200 for Cut&Tag) from Abcam; Wdr82 (99715; 1:1,000 for WB; 1:100 for Cut&Tag), DDX5 (9877T; 1:1,000 for WB), and RBBP5 (13171S; 1:200 for ChIP) from Cell Signaling Technology; PCBP2 (NBP2-19715; 1:1,000 for WB) from Novusbio; PTBP1 (32-4800; 1:1,000 for WB) from Invitrogen; α-globin (14537-1-AP; 1:2,000 for WB) from Proteintech; EKLF (OM184222; 1:1,000 for WB) and β-globin (OM256195; 1:1,000 for WB) from OmnimAbs; and ASH2L (A11278; 1:200 for ChIP) from Abclonal. Anti-FLAG M2 affinity gel (A2220), 3× FLAG peptide (F4799), doxycycline (D9891), PHZ (P26252) and 2′,7′-dichlorofluorescin diacetate (DCF-DA; 35845) were purchased from Sigma-Aldrich. Guinea pig anti-rabbit IgG (heavy & light chain) antibody (ABIN101961; 1:100 for Cut&Tag) was from Antibodies Online. Tetramethylrhodamine methyl ester (TMRE; I34361) and 2-NDBG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose; N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03), murine IL-3 (213-13), and recombinant human EPO (100-64) were obtained from PeproTech.

Yang S., Sun G., Wu P., Chen C., Kuang Y., Liu L., Zheng Z., He Y., Gu Q., Lu T., Zhu C., Wang F., Gou F., Yang Z., Zhao X., Yuan S., Yang L., Lu S., Li Y., Lv X., Dong F., Ma Y., Yu J., Ng L.G., Shi L., Liu J., Shi L., Cheng T, & Cheng H. (2022). WDR82-binding long noncoding RNA lncEry controls mouse erythroid differentiation and maturation. The Journal of Experimental Medicine, 219(4), e20211688.

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Western Blot Analysis of Protein Expression

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Cell populations were lysed using RIPA lysis buffer (containing PMSF, protease inhibitor cocktail, and sodium orthovanadate; Santa Cruz Biotechnology, Dallas, TX, USA), and protein concentrations were calculated using a BCA protein assay (Pierce Biotechnology, Rockford, IL, USA). After denaturation with Laemmli buffer (BioRad Laboratories, Hercules, CA, USA), 10 μg of total protein was loaded on 10% polyacrylamide SDS-PAGE gels, transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Billerica, MA, USA), and probed using the following antibodies: TLR4 (Santa Cruz Biotechnology, 1:1000), SOX2 (R&D Systems, 1:1000), RBBP5 (Cell Signaling, Danvers, MA, USA, 1:2000), GFAP (Invitrogen, 1:1000), TBK1 (Cell Signaling, 1:500), phospho-TBK1 (Cell Signaling, 1:500), IRAK1 (Abcam, 1:1000), phospho-IRAK1 (Abcam, 1:1000), MLL1 (Bethyl Laboratories, 1:1000), OCT4 (Cell Signaling, 1:1000), and NANOG (Cell Signaling, 1:1000); β-Actin (Santa Cruz Biotechnology, 1:2000) was used as a loading control. Species-specific horseradish peroxidase (HRP)-conjugated secondary antibodies were used for detection (Invitrogen, 1:5000). Membranes were developed using ECL-2 reagent (Pierce Biotechnology). All western blots were performed at least three times.

Alvarado A.G., Thiagarajan P.S., Mulkearns-Hubert E.E., Silver D.J., Hale J.S., Alban T.J., Turaga S.M., Jarrar A., Reizes O., Longworth M.S., Vogelbaum M.A, & Lathia J.D. (2017). Glioblastoma Cancer Stem Cells Evade Innate Immune Suppression of Self-Renewal through Reduced TLR4 Expression. Cell stem cell, 20(4), 450-461.e4.

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Western Blot Analysis of iPSC-CMs

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Human iPSC-CMs grown in 6-wells plates were harvested and lysed in RIPA buffer with Complete Mini, EDTA-free Protease inhibitor cocktail tablets (Roche). The lysates were placed on ice for 30 minutes, followed by centrifuging at 14000 rpm for 20 min, the supernatants were then collected as proteins. BCA Protein Assay kit (Thermo Fisher Scientific) was used to measure the protein concentration. Western blot was performed according to the standard protocol. Briefly, Equal amounts of protein was treated by SDSPAGE electrophoresis and transferred to a nitrocellulose membrane. After nonfat milk blocking, the membrane was incubated with the following primary antibodies at 4°C overnight, respectively: TLK1 (Fisher Scientific, 720397; 1:500 dilution), TEAD2 (Abcam, ab273017; 1:500 dilution), RBBP5 (Cell Signaling Technology, 12766S; 1:1000 dilution), ASH2L (Bethyl Laboratories, A300-107A-M; 1:500 dilution) and GAPDH (Abcam, ab8245; 1:1000 dilution). Subsequently, the membrane was incubated in protein-specific HRP conjugated secondary antibody for 1 hr at room temperature. The blots were visualized using chemiluminescence (Thermo Fisher Scientific).

Jessie Wang Y., Zhang X., Keung Lam C., Guo H., Wang C., Zhang S., Wu J.C., Snyder M, & Li J. (2022). Systems Analysis of de novo Mutations in Congenital Heart Diseases Identified a Protein Network in Hypoplastic Left Heart Syndrome. Cell systems, 13(11), 895-910.e4.

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