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Purified active cdk1 cyclin b1

Manufactured by Merck Group

Purified active Cdk1/cyclin B1 is a laboratory product that consists of the Cdk1 protein and the cyclin B1 protein in their active form. The core function of this product is to provide a source of active Cdk1/cyclin B1 complex for use in various research applications.

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2 protocols using purified active cdk1 cyclin b1

1

Cdk1/cyclin B1 Kinase Assay

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Purified active Cdk1/cyclin B1 was purchased from Millipore. Phosphocellulose P81 paper discs were purchased from Whatman. [γ-32P]ATP (250 µCi at 10 µCi/µl) was obtained from Perkin-Elmer. Precast 16.5% acrylamide Tris/Tricine gels, in an 18-well format, and peptide molecular mass standards were purchased from Bio-Rad. Lambda protein phosphatase was obtained from New England BioLabs. Other chemicals were obtained from Sigma.
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2

Cdk1/cyclin B1 Kinase Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified active Cdk1/cyclin B1 was purchased from Millipore. Phosphocellulose P81 paper discs were purchased from Whatman. [γ-32P] ATP (250 μCi at 10 μCi/μl) was obtained from Perkin-Elmer. Precast 16.5% acrylamide Tris/Tricine gels, in an 18-well format, and peptide molecular mass standards were purchased from Bio-Rad. Lambda protein phosphatase was obtained from New England BioLabs. Other chemicals were obtained from Sigma.
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