Skin tissue sections were incubated with two unconjugated primary antibodies, anti-CD31 (Cat#sc-1506, Santa Cruz Biotechnology, TX, USA) and anti-DA D2 receptor antibody (Cat#sc-9113, Santa Cruz Biotechnology) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-phospho-vascular endothelial growth factor receptor-2 (p-VEGFR-2) (Cat# ab5473, Abcam) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-nidogen 2 (NID2) (Cat# ab14513, Abcam) or anti-CD31 (Cat# sc-1506, Santa Cruz Biotechnology) and anti-Apelin (Cat# 11497–1-AP, Life Technologies, CA, USA) at 4°C overnight. All the antibodies were used at a dilution of 1:100). The corresponding fluorochrome-conjugated secondary antibodies were applied for 1 hour at room temperature on the next day, and DAPI was used to counterstain the nuclei. Slides were mounted and visualized under a confocal scanning microscope (FBV-1000; Olympus Corporation, Center Valley, PA) (6 (link), 13 (link)).
Anti apelin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-Apelin is a laboratory reagent used for the detection and quantification of the apelin protein in biological samples. Apelin is a peptide hormone involved in various physiological processes, and its measurement can provide insights into related pathologies. The Anti-Apelin product is designed to support research and analysis in fields such as cardiovascular biology, metabolic disorders, and neuroendocrinology.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti apelin
1
Multicolor Immunofluorescence Staining of Skin Tissue
2
Immunofluorescence Staining of Paraffin-Embedded Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin-embedded tissue was baked and deparaffinized before antigen retrieval. After 1 h of blocking with normal donkey serum, tissues were incubated with two unconjugated primary antibodies, anti-CD31 (2 μg/ml) (Cat# sc-1506, Santa Cruz Biotechnology,1:100) and anti-phospho-vascular endothelial growth factor receptor-2 (p-VEGFR-2) (7.5 μg/ml) (Cat# ab5473, Abcam, 1:100) or anti-endothelial cell-specific molecule 1 (ESM 1) (10 μg/ml) (Cat# ab103590, Abcam, 1:100) and anti-Apelin (1.3 μg/ml) (Cat# 11497-1-AP, Life Technologies, 1:100) at 4 °C overnight. The corresponding fluorochrome-conjugated secondary antibodies were applied for 1 h at room temperature on the next day, and DAPI was used to counterstain the nuclei. Slides were mounted and visualized under a confocal scanning microscope (FBV-1000; Olympus Corporation, Center Valley, PA) [22 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!