The chaperone activity of HSP18 in the presence or absence of ATP and its non-hydrolysable analog (ATP-γS) was determined with two client proteins: lysozyme and citrate synthase (CS). All assays were performed with the aid of Perkin Elmer Lambda 35 UV spectrophotometer.
Lambda 35 uv spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Lambda 35 UV spectrophotometer is a compact and versatile instrument designed for a wide range of UV-Vis spectroscopy applications. It features a dual-beam optical system, a wavelength range of 190 to 1100 nm, and a variable slit width for optimal performance. The Lambda 35 provides accurate and reliable measurements with a high signal-to-noise ratio and a photometric range of -4 to 4 Abs.
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2 protocols using lambda 35 uv spectrophotometer
1
Chaperone Activity of HSP18 in ATP Presence
2
Quantifying Anthocyanin Content in Extracts
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Monomeric anthocyanin content (MAC) was determined by the differential pH method described by Wrolstad [32 ]. Briefly, two aliquots of 500 µL of appropriately diluted extracts were diluted 1/5. One aliquot was diluted with pH 1.0 buffer (0.025 M potassium chloride) and the other was diluted with pH 4.5 buffer (0.4 M sodium acetate). Absorbance of diluted samples was measured at 510 and 700 nm using a Perkin–Elmer Lambda 35 UV spectrophotometer (Boston, MA, USA). MAC in the extracts was calculated according to the formula:
where A is the absorbance, 449.2 is the molecular mass of cyanidin-3-O-glucoside, 26,900 is its molar absorptivity (ε) and DF is the dilution factor. The results were expressed as µg cyanidin-3-O-glucoside equivalent (C3G)/g. All extracts were analysed in triplicate.
where A is the absorbance, 449.2 is the molecular mass of cyanidin-3-O-glucoside, 26,900 is its molar absorptivity (ε) and DF is the dilution factor. The results were expressed as µg cyanidin-3-O-glucoside equivalent (C3G)/g. All extracts were analysed in triplicate.
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