Anti human igg fc antibody coupled to hrp

The primary antibodies directed against FGFR1 (#9740), FGFR2 (#11835), FGFR3 (#4574), phospho-FGFR1 (pFGFR1; #3476), ERK1/2 (#9102), and phospho-ERK1/2 (pERK1/2; #9101) were from Cell Signaling (Danvers, MA, USA). The anti-tubulin primary antibody (#T6557) and the anti-GST antibody (#G1160) were from Sigma-Aldrich (St. Louis, MO, USA). Anti-human IgG (Fc) antibody coupled to HRP (#ab97225) was from Abcam (Cambridge, UK). The primary antibodies directed against FGFR4 (#sc-136988) and the anti-His-Tag primary antibodies (#sc-8036) were from Santa Cruz Biotechnology (Dallas, TX, USA). Secondary antibodies were obtained from Jackson Immuno-Research Laboratories (Cambridge, UK). Protein A Sepharose, Glutathione Sepharose, and Heparin Sepharose resins were from GE Healthcare (Chicago, IL, USA). Ni–NTA agarose and PrestoBlue™ Cell Viability Reagent (Thermo Fisher Scientific, Waltham, MA, USA). α-Lactose Separopore resin was from bioWORLD (Irving, TX, USA).

The primary antibodies: anti-phospho-FGFR (Tyr653/Tyr654) (p-FGFR) (#06-1433), anti-FGFR1 (FGFR1) (#9740), anti-phospho-p44/42 (Thr202/Tyr204) MAP kinase antibody (p-Erk1/2) (#9101), anti-p44/42 MAP kinase antibody (Erk1/2) (#9102), anti-caspase 3 (caspase 3) (#9662) and anti-poly [ADP-ribose] polymerase (PARP) (#9542) were from Cell Signaling Technology. The primary antibodies anti-FHF1 antibody (#PA5-67182) was from Thermo Fisher Scientific. Anti-tubulin primary antibody (#T6557) were from Sigma-Aldrich. Anti-human IgG (Fc) antibody coupled to HRP (#ab97225) was from Abcam. Horseradish peroxidase-conjugated secondary antibodies were from Jackson ImmunoResearch and chemiluminescent substrate was used for their visualization in the ChemiDoc station (BioRad). heparin Sepharose resin was from GE Healthcare. Geneticin (G-418) was from BioShop. PD173074 and heparin were from Sigma-Aldrich.