Goat polyclonal anti gfap antibody

The nuclear (Hoechst 33342) and lipophilic dyes (Fluoromyelin-green and Fluoromyelin-red) were purchased from Invitrogen (Carlsbad, CA, USA). The following antibodies were used in our study: rabbit polyclonal anti-Kv3.1, anti-Kv3.3 and anti-AQP4 antibodies (Alomone Labs, Jerusalem, Israel); rabbit polyclonal anti-BDNF and anti-pan-Trk antibodies (Santa Cruz Biotechnology, Dallas, TX); rabbit polyclonal anti-P75 antibody (a kind gift from Dr. Sung-Ok Yoon, The Ohio State University, Columbus, OH); rabbit polyclonal anti-TrkB (phospho-Y816) antibody (AbCAM, Cambridge, MA, USA); rat monoclonal anti-MBP antibody (Millipore, Billerica, MA); goat polyclonal anti-GFAP antibody (AbCAM); mouse monoclonal anti-Kv1.2, anti-Kv2.1 and anti-Ankyrin-G antibodies (Neuromab, Davis, CA); chicken polyclonal anti-Vimentin antibody (Millipore); Alexa488-, Alexa 647-, Dylight 488-, Dylight 649-, Cy3-, and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA). Myelin oligodendrocyte glycoprotein (MOG) peptide 35-55 (MEVGWYRSPFSRVVHLYRNGK) was purchased from Pro-Spec (Rehovot, Israel). Recombinant BDNF peptide (mature form) was purchased from Sigma-Aldrich (St. Louis, MO). Ground inactivated mycobacteria tuberculosis H37Ra and Incomplete Freund's Adjuvant were from Difco Laboratories (Detroit, MI, USA).

Curcumin was purchased from Cayman Chemical (Ann Arbor, MI, USA). Phosphate‐buffered saline (PBS, pH 7.4), paraformaldehyde (PFA), Triton X‐100 and BCA assay kits were purchased from Thermo Fisher Scientific (Carlsbad, CA, USA). Thioflavin‐S was purchased from Sigma‐Aldrich (St. Louis, MO, USA). Cell lysis buffer and phenylmethylsulfonyl fluoride (PMSF) were purchased from Beyotime Biotechnology (Shanghai, China). Primary antibodies used for immunofluorescence staining and Western blot, including goat polyclonal anti‐GFAP antibody, rabbit polyclonal anti‐COX2 antibody, rabbit monoclonal anti‐HMGB1 antibody, rabbit polyclonal anti‐RAGE antibody, mouse monoclonal anti‐TLR4 antibody, rabbit monoclonal anti‐NF‐κB p65 antibody and mouse polyclonal anti‐β‐actin antibody were purchased from Abcam (Cambridge, UK), Cell Signaling Technology (CST, Danvers, MA, USA) or Bioworld (St. Louis Park, USA). Secondary antibodies, including DyLight 488 AffiniPure Donkey Anti‐Goat IgG, HRP‐labelled rabbit anti‐goat, HRP‐labelled goat anti‐rabbit and HRP‐labelled goat anti‐mouse, were obtained from EarthOx (Millipore, Burlington, MA, USA) or Fdbio Science (Hangzhou, China).