Tagment dna enzyme from the nextera dna sample prep kit

ChIPmentation is compatible with various different protocols for ChIP, which makes it easy to apply ChIP-mentation to antibodies that work best with a certain ChIP protocol. The chosen ChIP protocol was followed until the beads carrying immunoprecipitated chromatin were washed with LiCl-containing wash buffer (WBIII for ChIP version 1, RIPA-LiCl for ChIP version 2, and TF-WBIII for ChIP version 3, as described in the Supplementary Note). Beads were then washed twice with 10 mM cold Tris-Cl pH 8.0 to remove detergent, salts, and EDTA. Subsequently, beads were resuspended in 30 μl of the tagmentation reaction buffer (10 mM Tris, pH 8.0, 5 mM MgCl₂) containing 1 μl Tagment DNA Enzyme from the Nextera DNA Sample Prep Kit (Illumina) and incubated at 37°C for 10 minutes in a thermocycler. Following tagmentation, the beads were washed twice with 150 μl cold WBI (ChIP version 1), RIPA (ChIP version 2), or TF-WBI (ChIP version 3). Afterward, the chosen ChIP protocol was resumed with the final bead wash, elution from beads, reverse-crosslinking, and DNA purification. A detailed protocol can be found at http://chipmentation.computational-epigenetics.org/.