Cardiac rhythm was monitored for about an hour in each mouse using surface electrodes while the mouse was kept under light anesthesia, as published[35 (link),36 (link)]. The rhythm was recorded using a Power Lab 4/30 data acquisition system and data were analyzed using Lab Chart7 software (ADInstruments, Colorado Springs, CO, USA).
Power lab 4 30 data
Manufactured by ADInstruments
Sourced in United States
The PowerLab 4/30 data acquisition system is a versatile laboratory equipment designed for recording and analyzing physiological signals. It features four analog input channels, a sampling rate of up to 200 kHz, and a resolution of 16 bits. The device is capable of connecting to a variety of sensors and transducers, allowing for the capture of various physiological measurements.
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Lab products found in correlation
5 protocols using power lab 4 30 data
1
Cardiac Rhythm Monitoring in Mice
2
Measuring Adipocyte Oxygen Consumption
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Isolated adipocyte OCR was measured using a Clark-type electrode digital model 10 controller with a constant stir bar (Rank Brothers) and was water jacketed to maintain adipocytes at 37 °C, and data was recorded by a PowerLab 4/30 data acquisition system (AD Instruments) as previously described with modifications [31] (link), [32] (link), [33] (link). The electrode was pre-calibrated with oxygenated water at 37 °C and then with 500 μL oxygenated KRB without cells to detect electrode drift. After the third wash with KRB as described above, isolated adipocytes were diluted 1:3 in KRB, and a 500 μL aliquot of adipocytes were transferred to the electrode chamber and air sealed. Basal respiration was recorded for 15 min and then NaCN was injected for a final concentration of 1 mM and measured for 10 min to inhibit oxygen consumption. Results were calculated based on the assumption that oxygenated water contains 225 μmol/l O2, and data were corrected for electrode drift and normalized for fat cell number calculated as described above. Isolated adipocytes from one ACSL4floxed and one Ad-KO littermate were isolated after 12 weeks on diet and oxygen consumption measured per day to minimize variability for a total of 4 subsequent days (n = 4 mice/genotype).
3
Cardiac Rhythm Monitoring in Mice
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Cardiac rhythm was monitored for about an hour in each mouse using surface electrodes while the mouse was kept under light anesthesia, as published[35 (link),36 (link)]. The rhythm was recorded using a Power Lab 4/30 data acquisition system and data were analyzed using Lab Chart7 software (ADInstruments, Colorado Springs, CO, USA).
4
Penile Cavernosal Tissue Isolation and Evaluation
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The animals were rendered unconscious by inhalation of CO2 and then decapitated. The penises were surgically removed and immediately placed in chilled Krebs solution (NaCl 118 mmol/L, NaHCO3 25 mmol/L, glucose 5.6 mmol/L, KCl 4.7 mmol/L, KH2PO4 1.2 mmol/L, MgSO4 1.17 mmol/L, and CaCl2 2.5 mmol/L). Cavernosal strips were obtained by dissection of the tunica albuginea and surrounding connective tissues. Each penis resulted in two strips, one for each CC, that were vertically suspended between two metal hooks in 10-mL organ baths containing Krebs solution at 37°C continuously bubbled with a mixture of 95% O2 and 5% CO2 (pH = 7.4). One hook was connected to a force transducer (ADInstruments, Colorado Springs, CO, USA) and the other acted as a fixed attachment point. The resting tension was adjusted to 4 mN and CC strips were allowed to equilibrate for 60 minutes.25 (link), 26 (link), 27 (link) Changes in isometric force were recorded using a PowerLab 4/30 data acquisition system (Software Chart, version 7.0; ADInstruments).
5
Sciatic Nerve Injury and Repair Evaluation
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