Ldh assay

Manufactured by Roche

Sourced in Germany, Switzerland, United States

The LDH assay is a laboratory test used to measure the activity of the enzyme lactate dehydrogenase (LDH) in a sample. LDH is present in various cells throughout the body and is released upon cell damage or death. The LDH assay provides a quantitative assessment of LDH levels, which can be used as an indicator of tissue or organ injury.

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Lab products found in correlation

Multiscan go, by Thermo Fisher Scientific (2 mentions) Microplate reader, by Agilent Technologies (2 mentions) Mts assay, by Promega (2 mentions) Alamarblue, by Bio-Rad (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Mouse il 1β ready set go kit, by Thermo Fisher Scientific (1 mentions) Multiskan spectrum, by Thermo Fisher Scientific (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Elisa reader, by Molecular Devices (1 mentions) Cell death detection elisa assay, by Roche (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Multimode reader, by Agilent Technologies (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts assay, by Promega (1 mentions) Live dead assay, by Thermo Fisher Scientific (1 mentions) Fluorescence microscope, by Leica (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

LDH-Cytotoxicity Assay Kit II LDH) cytotoxicity detection assay LDH) release assay LDH Cytotoxicity Assay Kit LDH assay kit Lactate dehydrogenase (LDH) release assay LDH activity assay Lactate dehydrogenase (LDH) cytotoxicity assay kit Cytotoxicity detection kit (LDH release assay) Cytotoxicity Detection Kit (LDH assay)

28 protocols using ldh assay

Plasma LDH Activity Quantification

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Blood samples were collected after anesthetization with isoflurane and centrifuged at 3500 rpm for 15 min at 4 °C. Plasma LDH activity, a marker for cytotoxicity, was measured using a commercial LDH assay according to the manufacturer’s instructions (Roche Molecular Biochemicals, Mannheim, Germany).

Kim J.R., Kang P., Lee H.S., Kim K.Y, & Seol G.H. (2017). Cardiovascular effects of linalyl acetate in acute nicotine exposure. Environmental Health and Preventive Medicine, 22, 42.

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Cytokine and Cell Death Assays

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Supernatants were harvested at various time points for cytokine measurement and in select experiments for the lactate dehydrogenase (LDH) assay (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol. The percentage of LDH release, as a measure of cell death, was calculated as follows: (LDH infected - LDH control)/(LDH total lysis - LDH control) × 100. The remaining supernatants were stored at -20 °C for use in an IL-1β enzyme-linked immunosorbent assay (ELISA) by a mouse IL-1β Ready-Set-Go Kit (eBioscience, San Diego, USA).

Wang X., Gong P., Zhang X., Wang J., Tai L., Wang X., Wei Z., Yang Y., Yang Z., Li J, & Zhang X. (2017). NLRP3 inflammasome activation in murine macrophages caused by Neospora caninum infection. Parasites & Vectors, 10, 266.

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Quantifying Pyroptosis via LDH Assay

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Pyroptosis was analyzed by detecting the activity of LDH released into the cell supernatants (collected from 3 × 10⁶ cells) at different treatment for 24 or 36 h using an LDH assay (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s protocol. The absorbance was measured at 490 nm. The percentage of LDH release was calculated as follows: (LDH_infected − LDH_control)/ (LDH_{total lysis} − LDH_control) × 100.

Li L., Wang X.C., Gong P.T., Zhang N., Zhang X., Li S., Li X., Liu S.X., Zhang X.X., Li W., Li J.H, & Zhang X.C. (2020). ROS-mediated NLRP3 inflammasome activation participates in the response against Neospora caninum infection. Parasites & Vectors, 13, 449.

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Necroptosis Inhibition in HepG2 Cells

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HepG2 cells were pretreated with 20 μM z-VAD-fmk or 50 μM Nec-1 for 30 min, and treated with 5 or 10 μg/ml Tan IIA for 1–36 h. LDH assay (Roche Co.) was performed as per manufacturer’s instructions. The absorbance was measured in a microplate reader (Thermo Scientific Multiskan Spectrum, New York, NY, USA) at 490 nm. Microsoft Excel 2010 was used for all data entry and analysis. Data were presented as mean±S.E. of the quadruplicate. Student's two-tailed t-test was used to analyze statistical significance between groups.

Lin C.Y., Chang T.W., Hsieh W.H., Hung M.C., Lin I.H., Lai S.C, & Tzeng Y.J. (2016). Simultaneous induction of apoptosis and necroptosis by Tanshinone IIA in human hepatocellular carcinoma HepG2 cells. Cell Death Discovery, 2, 16065-.

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Lactate Dehydrogenase Assay Protocol

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LDH assay (4744926001, Roche, Penzberg, Germany) was performed according to the manufacturer’s instructions and evaluated in the ELISA Reader (Multiscan GO, Thermo Fisher Scientific, Waltham, MA, USA).

Paddenberg E., Osterloh H., Jantsch J., Nogueira A., Proff P., Kirschneck C, & Schröder A. (2022). Impact of Leptin on the Expression Profile of Macrophages during Mechanical Strain In Vitro. International Journal of Molecular Sciences, 23(18), 10727.

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Cytotoxicity Assessment Using LDH Assay

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An LDH assay (04744926001, Roche, Penzberg, Germany) was performed according to the manufacturer’s instructions to assess cytotoxicity.

Paddenberg E., Forneck A., Widbiller M., Smeda M., Jantsch J., Proff P., Kirschneck C, & Schröder A. (2022). Impact of Melatonin on RAW264.7 Macrophages during Mechanical Strain. International Journal of Molecular Sciences, 23(21), 13397.

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Cytotoxicity Assays for Neuronal Cultures

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The MTS assay (Promega Corporation, Madison, WI) and LDH assay (Roche Life Science, Indianapolis, IN) were performed according to the manufacturer’s instructions. Neurons were grown in 96-well plates at a density of 10,000 cells/well for 7 days. Different treatments were then added to the culture medium for designated time periods. The culture medium was removed for the LDH assay and cells were left in the wells for the MTS assay. For both assays, the absorbance (optical density) value was read at a wavelength of 492 nm with a microplate reader (Biotek, Winooski, VT).

Zhang S., Eitan E., Wu T.Y, & Mattson M.P. (2017). Intercellular transfer of pathogenic α-synuclein by extracellular vesicles is induced by the lipid peroxidation product 4-hydroxynonenal. Neurobiology of aging, 61, 52-65.

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Murine C3a Effects on Cell Death

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Cell death was assessed by LDH assay according to the manufacturer’s instructions (Roche Products). Serial concentrations of murine C3a (R&D Systems, 0-100nM) were incubated with PCN and bEnd.3 cells subjected to OGD followed by 24h re-oxygenation. The reaction mixture (100 μl) was added to conditioned media (100μl) removed from culture wells. Sample absorbance was measured (Synergy H1, BioTek) at 490nm after 15 minutes of room temperature incubation. The same volume of blank medium was used as background control.

Zhao X.J., Larkin T.M., Lauver M.A., Ahmad S, & Ducruet A.F. (2017). Tissue plasminogen activator mediates deleterious complement cascade activation in stroke. PLoS ONE, 12(7), e0180822.

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Evaluating Cytotoxicity and Antiviral Effects of EBS

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Human microglial CHME3 cells [65 (link)] were grown in DMEM containing 10% fetal bovine serum. Cytotoxicity of EBS was determined by using lactate dehydrogenase (LDH) assay (Roche). Briefly, cells treated with EBS at the indicated doses for overnight underwent LDH assay. Sample absorbance was determined by using an ELISA reader (molecular device) at 490 nm. For antiviral study, cells in 12-well plates were adsorbed with ZIKV at 0.1 of multiplicity of infection for 2 hours with the indicated doses of EBS, washed thoroughly to remove unbound viruses, then incubated for another 24 hours in the presence or absence of EBS. The antiviral effect of EBS was evaluated by immunofluorescence and plaque-forming assays.

Simanjuntak Y., Liang J.J., Chen S.Y., Li J.K., Lee Y.L., Wu H.C, & Lin Y.L. (2018). Ebselen alleviates testicular pathology in mice with Zika virus infection and prevents its sexual transmission. PLoS Pathogens, 14(2), e1006854.

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Cell Viability and Cytotoxicity Assays

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Cells were cultured for 24 h, after which the medium (lactate dehydrogenase (LDH) assay, Roche) and cells (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MTT assay) were analyzed as described [16 (link)].

Sikkema A.H., Stoffels J.M., Wang P., Basedow F.J., Bulsink R., Bajramovic J.J, & Baron W. (2018). Fibronectin aggregates promote features of a classically and alternatively activated phenotype in macrophages. Journal of Neuroinflammation, 15, 218.

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