Enhanced chemical luminescence

Manufactured by Sartorius

Sourced in Israel

Enhanced chemical luminescence is a laboratory equipment that produces light through a chemical reaction. It is a sensitive and precise tool used for various applications in scientific research and analysis.

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Lab products found in correlation

Lysis buffer, by Thermo Fisher Scientific (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Bis tris polyacrylamide gel, by Thermo Fisher Scientific (1 mentions) Primary antibodies against caspase 1, by Abcam (1 mentions) Hrp conjugated second antibodies, by Merck Group (1 mentions) Pdvf membrane, by Merck Group (1 mentions) Ripa buffer, by Santa Cruz Biotechnology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Sphk2, by Cell Signaling Technology (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Ripa buffer, by Beyotime (1 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)

3 protocols using enhanced chemical luminescence

Quantification of Caspase-1 in Liver Tissue

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Liver tissues were homogenized in chilled lysis buffer (Thermo Fisher Scientific, Waltham, MA, USA), followed by incubation for 20 minutes on ice. After centrifugation at 12000 g for 10 minutes at 4°C, the supernatants were collected as protein samples. Protein content was determined using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Approximately 40 μg of protein was loaded into a 12% bis-tris polyacrylamide gel (Thermo Fisher Scientific, Waltham, MA, USA) for separation. Afterward, the proteins were transferred to a polyvinyl difluoride membrane. After blocking, the membranes were blotted with primary antibodies against caspase-1 (Abcam, San Francisco, CA, USA), which was followed by blotting with a secondary antibody in a standard fashion. The membranes were developed using enhanced chemical luminescence (Biological Industries, Kibbutz Beit-Haemek, Israel) and exposed to X-ray films. β-Actin served as a protein loading control.

Chen Q., Yang Y., Pan Y., Shen L., Zhang Y., Zheng F., Shu Q, & Fang X. (2022). Human Neutrophil Defensins Disrupt Liver Interendothelial Junctions and Aggravate Sepsis. Mediators of Inflammation, 2022, 7659282.

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Western Blot Analysis of SphK1 and SphK2

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Samples were lysed in RIPA buffer (Santa Cruz Biotechnology, Santa Cruz, CA) and 15 μg of total lysate was resolved with 12% SDS-PAGE. Proteins were then transferred to PDVF membranes (Millipore, Billerica, MA). Membranes was blocked with 5% fat-free milk/PBS and blotted with antibodies against SphK1, Sphk2, or β-actin (Cell Signaling, Boston, MA). Membranes were subsequently washed and incubated in HRP-conjugated second antibodies (Millipore, Billerica, MA), washed, and detected with enhanced chemical luminescence (Biological Industries, Kibbutz Beit-Haemek, Israel) and exposed to X-ray films. The resulting protein bands were analyzed with Image J software.

Wu X., Hou J., Li H., Xie G., Zhang X., Zheng J., Wang J., Gao F., Yao Y., Liu H, & Fang X. (2019). Inverse Correlation Between Plasma Sphingosine-1-Phosphate and Ceramide Concentrations in Septic Patients and Their Utility in Predicting Mortality. Shock (Augusta, Ga.), 51(6), 718-724.

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Alveolar Macrophage Ferroportin Expression

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Alveolar macrophages were isolated as described above. Then, the samples were lysed in RIPA buffer (Beyotime Biotechnology, Shanghai, China), and 30 μg of total lysate was resolved with 12% SDS-PAGE. The proteins were then transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, Mass). The membrane was blocked with 5% non-fat milk and blotted with anti-mouse ferroportin antibody (Alpha Diagnostic Intl, San Antonio, Tex), followed by HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, Pa). The membranes were developed using enhanced chemical luminescence (Biological Industries, Kibbutz Beit-Haemek, Israel) and exposed to X-ray films. β-actin served as a protein loading control.

Yang Y., Zeng C., Yang S., Zhang Y., Song S., Liu S., Shu Q., Fang X, & Chen Q. (2020). Airway Epithelial Hepcidin Coordinates Lung Macrophages and Immunity Against Bacterial Pneumonia. Shock (Augusta, Ga.), 54(3).

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