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Nano zse

Manufactured by Malvern Panalytical
Sourced in United Kingdom

The Nano ZSE is a laboratory instrument designed for the analysis of particle size and zeta potential. It utilizes dynamic light scattering (DLS) and electrophoretic light scattering (ELS) techniques to measure the size and surface charge of particles suspended in liquid media. The instrument provides accurate and reliable data on the physical characteristics of nanoscale materials.

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11 protocols using nano zse

1

Functionally Modified Fly Ash Synthesis

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Raw FA was firstly modified by ball milling (FA-I) followed by the NaOH hydrothermal (130 °C) and H2SO4 etching processes (FA-II). Quaternary ammonium groups were then introduced by either physically covering the ash with cationic surfactants CTAB (FA-CSC) or silane coupling methods with TTPAC (FA-SCA). Various dosage of TTPAC were evaluated such as 30 wt% and 100 wt% of the modified FA (denoted as FA-SCA30 and FA-SCA100, respectively). Details of modification methods were included in SI section 1.
Surface morphology was characterized using a scanning electron microscope (SEM, ZEISS GeminiSEM 500). The introduced quaternary ammonium was validated using an attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR, PerkinElmer). The Brunauer–Emmett–Teller surface area (SBET) and pore volume (Vpore) were analyzed by a BET analyzer (MicrotracBEL, Belsorp max). The size distribution (via dynamic light scattering, DLS) and zeta potential were carried out using a Zetasizer (Malvern, Nano ZSE) with the refractive index of 1.45. The percentile values (D10, D50 and D90) were measured to indicate the cumulative 10%, 50% and 90% point of diameter in volume distribution, respectively. The volume mean diameter, as well as the span ((D90-D10)/D50), were calculated for a better description of particle size and distribution.
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2

Characterization of XBSD Nanoparticles

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One drop of XBSD and N-XBSD, was spread uniformly on a copper mesh coated with carbon film, respectively, dried, stained with 2.0% phosphotungstic acid for 2 min, dried again and the morphology of the nanoparticles was observed under transmission electron microscopy (TEM, Tokyo, Japan). A Zetasizer Nano ZSE (Malvern Zetasizer Nano-ZS ZEN 3600, Marvin, England) was used to determine the particle size distribution and surface potential of N-XBSD.
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3

Characterization of FK506 Liposomes

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The particle size, zeta potential, and polydispersity (PDI) of FK506 liposomes and blank liposomes were determined using a Malvern Nano-ZSE laser particle size analyzer. The liposomes were diluted 100 times, followed by gentle vibration before measurement.
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4

Comprehensive Multimodal Characterization

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Fluorescence spectra were measured by LS55 fluorescence spectrophotometer (PerkinElmer). Absorption spectra of Ultraviolet-visible (UV-Vis) were recorded using Lambda-35 UV-vis spectrophotometer (PerkinElmer). The transmission electron microscopy (TEM) and high-resolution TEM (HRTEM) images were analyzed using JEM-1400Plus transmission electron microscope (Japan Electron Optics Laboratory Co., Ltd). Zeta potentials for CDs were evaluated by zetasizer (Nano ZSE, Malvern Instruments, UK). VG Multilab 2000 X-ray photoelectron spectroscopy was gathered by surface analysis. Optical density (OD) in the cell was calculated using microplate reader (Thermo Scientific, England, UK). The circular dichroism spectra were gathered using Chirascan Plus spectropolarimeter (Applied Photophysics Ltd.). Scanning electron microscope (SEM) images for bacteria were conducted using Zeiss SIGMA scanning electron microscope (Carl Zeiss Jena). Fluorescence optical microscope (Nikon ECLIPSE Ti) was applied on images through laser-scanning confocal fluorescence.
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5

Comprehensive Materials Characterization

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All samples were characterized by X-ray diffraction (XRD, Rigaku DMX-2550/PC, operating with Cu Kα radiation (λ = 1.5418 Å) at a scanning rate of 10° min−1 in a 2θ range from 5° to 70°), Fourier transform infrared (FT-IR) spectrum (measured on a Tensor27 infrared spectrometer (Bruker) using KBr disks), and thermal analysis (TG-DSC, performing on a TGA/DSC3+, METTLER thermal analyzer under a nitrogen atmosphere with a heating rate of 10 °C min−1 over the range from 30 to 800 °C). The morphologies of the samples were observed by field emission scanning electron microscopy (FSEM, SU-8020, Hitachi). The porosity and the Brunauer–Emmett–Teller (BET) specific surface area of samples were measured by the nitrogen adsorption/desorption at 77.3 K by using surface analytical instrument (America Micromeritics ASAP 2460. The sample had been degassed at 120 °C for 6.0 h under vacuum of 10−5 bar), and the pore size distribution was evaluated from the N2 desorption isotherm using the Barrett–Joyner–Halenda (BJH) model. The zeta potentials were measured with a dynamic light scattering instrument (Nano-ZSE, Malvern) at 90°.
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6

Characterization of Self-Assembled Nanoparticles

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The size, particle size distribution and surface potential of the self-assembled nanoparticles were determined using a Zetasizer Nano ZSE (Malvern Zetasizer Nano-ZS ZEN 3600, Marvin, England). The infrared spectra of monomers, physical mixtures and nanoparticles in the range of 4000–400 cm−1 were determined by FTIR spectroscopy (Thermo, Florida, USA). HRMS (Agilent 6545 LC/Q-TOF, Agilent Technologies, Santa Clara, CA, USA) was used to analyze the basic units of two self-assembled nanoparticles.
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7

Characterization of CaCO3@Cur@QTX125@HA Nanoparticles

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CaCO3@Cur@QTX125@HA nanoparticles were suspended in 1.5 mL of deionized water and thoroughly vortexed. The size and zeta potential of the nanoparticles were measured using a Malvern Instruments Zetasizer (Nano ZSE, He–Ne laser, λ = 632 nm) at a scattering angle of 173° and a temperature of 25 ºC. All measurements were repeated at least three times.
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8

Characterization of Reduction-Responsive Nanoparticles

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1H nuclear magnetic resonance (1H NMR) spectra were measured on a NMR spectrometer (AVANCE II 500 ​MHz, Bruker) using dimethyl sulfoxide-d6 (DMSO-d6) as the solvent. The high-resolution mass spectrum (HRMS) was measured on a Q Exactive UHMR Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific). Fourier transform infrared (FT-IR) spectra were measured on a FT-IR spectrometer (iS50, Thermo) by a standard KBr disk method. Ultraviolet–visible (UV–Vis) spectra were measured on a microplate reader (Spark, Tecan). Thermal analysis was performed on a high sensitivity differential scanning calorimeter (DSC, DSC7020, Hitachi) with a closed aluminum pan system over the temperature of 30–300 ​°C. The DSC analysis was operated at a heating rate of 10 ​°C/min. The morphologies of the prepared nanoparticles were observed with transmission electron microscope (TEM, JEM-2100, JEOL) operated at an accelerating voltage of 200 ​KV. To investigate the reduction-responsiveness of the prepared nanoparticles, the nanoparticles were treated with 10 ​mmol/L of GSH for 24 ​h and the morphologies were observed with TEM. The samples were negatively stained with 2% phosphotungstic acid (w/w). The size and zeta potential of the prepared nanoparticles were measured by dynamic light scattering (DLS, Nano ZSE, Malvern).
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9

Characterization of Graphene Sulfur Particles

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The UV-visible absorption spectra were recorded on a UV-visible spectrophotometer (G10S, Thermo Fisher, Waltham, MA). The size distribution of GSP was measured on a dynamic light scattering (DLS) analyzer (Malvern Nano ZSE, London, UK). The morphology of GSP was characterized on a high-resolution
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10

Characterizing CN/BD Nanoparticle Size and Charge

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The particle size and zeta potential of CN/BD nanoparticles were estimated using Nano-ZSE (Malvern Instruments) (Liu et al., 2022) (link). The nanoparticle dispersions were diluted 10 times and the pH of the dispersions was adjusted to 5.5 using a 2% phytic acid solution.
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