MS and MS/MS analysis of the spots on the MALDI plate were performed using a AB SCIEX TOF/TOFTM 5800 system. After data acquisition on each LC/MALDI sample spot in the MS mode, fractionation of automatically selected precursors (precursor selection criterion: S/N > 20; 250 m/z to 850 m/z; CCA matrix and adduct ions excluded with a tolerance of ±0.01 m/z units; maximum of 100 precursor ions from each spot) was performed in a negative 1 kV mode with air (~ 1 × 10−6 Torr) as the collision gas. The original MS data, stored in the TOF/TOF data base, was processed with Peak Explorer (AB Sciex).
Tof toftm 5800 system
Manufactured by AB Sciex
The TOF/TOFTM 5800 system is a high-performance mass spectrometer that utilizes time-of-flight (TOF) technology. It is designed to provide accurate and reliable mass analysis for a wide range of applications. The system features a dual-source configuration, allowing for both MALDI and ESI ionization techniques, which enables the analysis of a diverse range of sample types. The TOF/TOFTM 5800 system delivers high mass resolution and mass accuracy, making it a versatile tool for various analytical tasks.
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Lab products found in correlation
4 protocols using tof toftm 5800 system
1
MALDI-TOF/TOF Data Acquisition Protocol
2
Self-Assembled Peptide Nanostructures Characterization
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MALDI-TOF mass spectrometry at the Proteomics facility, Institute of Life Sciences, Bhubaneswar (AB SCIEX TOF/TOFTM 5800 System) was used to determine the mass of the peptides of the purified peptides and the matrix CHCA was used, and the matrix mixed peptides were dropped in the MALDI plate and given time to dry. The self-assembled nanostructure of 2 mM WR and WH series of peptides was prepared in an aqueous solution and kept for 2 days at 25°C–30°C. Ten-microliter samples were placed into the 300-mesh formvar-coated carbon TEM grid and incubated for 2 min. The peptides were stained with 20 μL of 2% uranyl acetate applied to the TEM grid and incubated for 2 min at room temperature. The additional stain was removed using filter paper, and TEM grids were kept at room temperature for 24 h to completely dry. The images were taken with a field emission gun transmission electron microscope 300 kV (using the facility at IISER, Mohali).
3
MALDI-TOF Mass Spectrometry of Lipids
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Mass spectrometric measurements were performed using a MALDI-TOF mass spectrometer (ABSCIEX TOF/TOF TM5800 system) in reflector mode. The lipid sample (0.2 L) was applied to the metal sample plate, which was then inserted into the MALDI-TOF MS analyzer. The following parameters were used for analysis: negative ion mode, 1600 shots / spectrum and 3800 4600 absorption laser intensity. In addition, measurements were made in the MS/MS mode at 5600 absorption laser intensity.
4
Mass Spectrometry Imaging of Rat Tissue
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MS imaging analyses were performed using a TOF/TOF TM 5800 System (AB SCIEX) . Analyses were performed in positive-ion mode for masses in the range of m/z 450-1000, and in negative-ion mode for masses in the range of m/z 200-1800. The matrices for ionization used 50 mg/mL 2,5-dihydroxybenzoic acid in methanol/water (8:2, v/v) for positive ion mode and 20 mg/mL 9-aminoacrine in ethanol/ water (8:2, v/v) for negative ion mode. The ion images were constructed using Datacube Explorer software 6) . The MS parameters were set to obtain the highest sensitivity. Cryosections for MS imaging were prepared from three independent rats (n＝3) .
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