Deer mouse immune gene expression profiling has been previously described [21 (link)]. Briefly, primers (S2 Table ) for various immune genes were used to amplify cDNA collected from lungs using QuantiNova reverse transcription and SYBR Green I PCR kits (Qiagen). The ΔΔCq method was used with normalization within sample on GAPDH (ΔCq) and fold-change calculated for each gene against the means of the 3 sham inoculated control deer mice (ΔΔCq). Statistical assessment was performed using Kruskal-Wallis test (ANOVA) with Prism software (GraphPad, Inc.)
Sybr green 1 pcr kits
Manufactured by Qiagen
SYBR Green I PCR kits are a type of real-time PCR reagent used for the detection and quantification of DNA or RNA targets. The kits contain SYBR Green I, a fluorescent dye that binds to double-stranded DNA, allowing for the monitoring of the amplification process in real-time.
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Lab products found in correlation
2 protocols using sybr green 1 pcr kits
1
Deer Mouse Immune Gene Expression
2
Deer Moue Immune Gene Expression
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Deer moue immune gene expression profiling has been previously described (19 (link)). Briefly, primers (Table S2 ) for various immune genes were used to amplify cDNA collected from lungs using QuantiNova reverse transcription and SYBR Green I PCR kits (Qiagen). The ΔΔCq method was used with normalization within sample on GAPDH (ΔCq) and fold-change calculated for each gene against the means of the 3 sham inoculated control deer mice (ΔΔCq).
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