Nasal swabs were obtained at research clinic visits. When participants were symptomatic, the parents were instructed to collect a swab at home (FLOQSwabsTM and UTMTM Viral Transport Media, COPAN Diagnostics, Murrieta, CA). Parents were trained in collection techniques by research personnel in clinic. Viral analysis was performed at the Special Projects Laboratory at Washington University. Respiratory virus polymerase chain reaction (PCR) was performed using the GenMark eSensor Respiratory Viral Panel using a research protocol that allowed identification of the following viruses: influenza A, influenza A 2009 H1N1, influenza B, respiratory syncytial virus A and B, parainfluenza viruses 1-4, human rhinovirus, human metapneumovirus, coronaviruses OC43, 229E, NL63, and HKU1, adenovirus B/E, adenovirus C (GenMark Diagnostics Inc. Carlsbad, CA).
Utmtm viral transport media
Manufactured by Copan
The UTMTM Viral Transport Media is a product designed to collect, preserve, and transport clinical specimens for laboratory testing. It provides a balanced salt solution that maintains the viability of viruses, bacteria, and other microorganisms during collection, storage, and transport to the laboratory.
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Lab products found in correlation
2 protocols using utmtm viral transport media
1
Respiratory Virus Detection Protocol
2
Respiratory Virus Detection in Children
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Samples were prospectively collected from 2-yr-old children (interquartile range 1.25–3.86) presenting with acute respiratory infection to the Princess Margaret Hospital Emergency Department as detailed in Table S1. Samples were obtained from previously healthy children as part of an influenza vaccine effectiveness study (Blyth et al., 2014 (link), 2016 (link)). Ethical approval for the study was obtained from the ethics committees of Princess Margaret Hospital for Children (1761EP), the South Metropolitan Area Health Service, and the Western Australian Aboriginal Health Information and Ethics Committee. Bilateral mid-turbinate nasal swabs were collected using flocked swabs (Copan Diagnostics). The swab was gently inserted into the nostril until resistance was felt. The swab was rotated several times against the nasal wall. On removal, swabs were inserted into a commercial viral transport media (UTMTM Viral Transport Media; Copan Diagnostics). All samples were frozen at −80°C. Nasopharyngeal samples were tested by PCR for respiratory viruses including RSV.
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