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Cd27 fitcor apc

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CD27 FITC or APC is a lab equipment product that detects the presence and expression level of the CD27 cell surface marker. CD27 is a member of the tumor necrosis factor receptor superfamily and is involved in the regulation of immune responses.

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Lab products found in correlation

Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (2 mentions) Zombie aqua, by BioLegend (2 mentions) Cd38 fitc, by BD (2 mentions) Cd19 pe cy7, by BD (2 mentions) 7 aad, by Thermo Fisher Scientific (2 mentions) Pd 1 pe, by Thermo Fisher Scientific (2 mentions) Fortessa cytometer, by BD (2 mentions) Cd4 apccy7 rpa t4, by BD (2 mentions) Cd25 biotin, by Thermo Fisher Scientific (2 mentions) Bcl6 alexa 647, by BD (2 mentions) Icosl apc, by BioLegend (2 mentions) Fas pe cf594, by BD (2 mentions) Cd40 apccy7, by BioLegend (2 mentions) Baffr pecy7, by BioLegend (2 mentions) Il21r bv421, by BioLegend (2 mentions) Humantrustain fcx, by BD (2 mentions) Cd127 fitc, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

CD27-APC (34 citations)

2 protocols using cd27 fitcor apc

1

Multiparameter Analysis of Tonsillar Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tonsillar lymphocytes were stained with the following anti-human
antibodies – CD4 APCCy7 (RPA-T4, BD Biosciences), CXCR5 Alexa 488 or
Alexa 647 (RF8B2, BD Biosciences), PD-1 PE (MIH4, eBioscience) or BV605 or BV421
(EH12.2H7, BioLegend), CD127 FITC (11-1278, eBioscience) or BV 421 (A019D5,
BioLegend), CD25 biotin (BC96, eBioscience or BioLegend) or PE-Cy7 (BC96, BD
Biosciences or BioLegend), BCL6 Alexa 647 or PE-Cy7 (K112-91, BD Biosciences),
CD3 APC (HIT3a, BD Biosciences) or Alexa 700 (UCHT1, BD Biosciences), CD27 FITC
or APC (M-T271, BD Biosciences), CD38 FITC (HIT2, BD Biosciences) or PE (HB7, BD
Biosciences), ICOSL APC (2D3, BioLegend), FAS PE-CF594 (DX2, BD Bioscience),
CD40 APCCy7 (5C3, BioLegend), BAFFR PECy7 (11C1, BioLegend), CD19 PECy7 or BV605
(SJ25C1, BD Bioscience), IL21R BV421 (17A12, BioLegend), CD86 BV421 (2331/FUN-1,
BD Bioscience). All surface stains were performed in the presence of Human
TruStain FcX (cat. 422302, BD Bioscience). Intracellular staining was performed
using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience) according
to the manufacturer’s instructions. Cells were stained with primary
antibodies followed by secondary reagents for 30 min at 4 °C. Data were
collected on a LSRII or Fortessa cytometer (BD) and analysed with FlowJo
software (TreeStar). 7-AAD (Invitrogen) or Zombie Aqua (BioLegend) staining was
used to exclude dead cells from analysis.
Papa I., Saliba D., Ponzoni M., Bustamante S., Canete P.F., Gonzalez-Figueroa P., McNamara H.A., Valvo S., Grimbaldeston M., Sweet R.A., Vohra H., Cockburn I.A., Meyer-Hermann M., Dustin M.L., Doglioni C, & Vinuesa C.G. (2017). TFH-derived dopamine accelerates productive synapses in germinal centres. Nature, 547(7663), 318-323.
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2

Multiparameter Analysis of Tonsillar Lymphocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tonsillar lymphocytes were stained with the following anti-human
antibodies – CD4 APCCy7 (RPA-T4, BD Biosciences), CXCR5 Alexa 488 or
Alexa 647 (RF8B2, BD Biosciences), PD-1 PE (MIH4, eBioscience) or BV605 or BV421
(EH12.2H7, BioLegend), CD127 FITC (11-1278, eBioscience) or BV 421 (A019D5,
BioLegend), CD25 biotin (BC96, eBioscience or BioLegend) or PE-Cy7 (BC96, BD
Biosciences or BioLegend), BCL6 Alexa 647 or PE-Cy7 (K112-91, BD Biosciences),
CD3 APC (HIT3a, BD Biosciences) or Alexa 700 (UCHT1, BD Biosciences), CD27 FITC
or APC (M-T271, BD Biosciences), CD38 FITC (HIT2, BD Biosciences) or PE (HB7, BD
Biosciences), ICOSL APC (2D3, BioLegend), FAS PE-CF594 (DX2, BD Bioscience),
CD40 APCCy7 (5C3, BioLegend), BAFFR PECy7 (11C1, BioLegend), CD19 PECy7 or BV605
(SJ25C1, BD Bioscience), IL21R BV421 (17A12, BioLegend), CD86 BV421 (2331/FUN-1,
BD Bioscience). All surface stains were performed in the presence of Human
TruStain FcX (cat. 422302, BD Bioscience). Intracellular staining was performed
using the FOXP3/Transcription Factor Staining Buffer Set (eBioscience) according
to the manufacturer’s instructions. Cells were stained with primary
antibodies followed by secondary reagents for 30 min at 4 °C. Data were
collected on a LSRII or Fortessa cytometer (BD) and analysed with FlowJo
software (TreeStar). 7-AAD (Invitrogen) or Zombie Aqua (BioLegend) staining was
used to exclude dead cells from analysis.
Papa I., Saliba D., Ponzoni M., Bustamante S., Canete P.F., Gonzalez-Figueroa P., McNamara H.A., Valvo S., Grimbaldeston M., Sweet R.A., Vohra H., Cockburn I.A., Meyer-Hermann M., Dustin M.L., Doglioni C, & Vinuesa C.G. (2017). TFH-derived dopamine accelerates productive synapses in germinal centres. Nature, 547(7663), 318-323.
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