Thiazolyl blue tetrazlium bromide mtt

The RL95-2 (ATCC^®CRL-1671™) and HEC-1-A (ATCC^®HTB-112™) human endometrial carcinoma cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). RL95-2 cells were cultured in Dulbecco’s modified Eagle’s medium nutrient mixture F-12 (DMEM/F12) supplemented with 10% fetal bovine serum (FBS), 0.005 mg/mL insulin, and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). HEC-1-A cells were cultured in McCoy’s 5A medium supplemented with 10% FBS and 1% penicillin–streptomycin. Doxorubicin, cisplatin, sodium palmitate, 2′,7-dichlorofluorescein diacetate (DCFH-DA), propidium iodide (PI), and thiazolyl blue tetrazlium bromide (MTT) were obtained from Sigma Aldrich (St. Louis, MO, USA).

HeLa and HEK293 (human embryonic kidney) cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM). A549 (human pulmonary adenocarcinoma) and Beas-2B (human bronchial epithelial) cells were cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium. HEC-1-A (human endometrial adenocarcinoma) cells were cultivated in McCoy’s 5A medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Thermo Fisher Scientific, Waltham, MA, USA). N-acetyl cysteine (NAC), cobalt (II) chloride, copper (II) chloride, 2′,7-dichlorofluorescein diacetate (DCFH-DA), MG132, propidium iodide (PI), nickel sulfate, and thiazolyl blue tetrazlium bromide (MTT) were obtained from Sigma Aldrich (St. Louis, MO, USA). Copper (I) chloride was from Alfa Aesar (Ward Hill, MA, USA). Copper sulfate was from Serva (Heidelberg, Germany). LY294002, PD98059, and SB203580 were from Millipore (Burlington, MA, USA).