Total RNA was extracted by using miRNAeasy Mini Kit from QIAGEN (217004). cDNA was synthesized by using the cDNA supermix kit (101414–106) and was amplified by using power SYBR green RT-PCR reagents kit from Quantabio (101414–276) with the condition 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds, 60°C for 30 seconds, and 72°C for 30 seconds, on a qPCR Instrument (Quantabio). All RT-PCR was performed in quadruplicate and the average fold changes were calculated based on 18S in the threshold cycle (Cq). Primer sequences are in the Table 1 (Supplementary Table S1).
Qpcr instrument
Manufactured by Quantabio
Sourced in United States
The QPCR Instrument is a specialized laboratory equipment used for quantitative polymerase chain reaction (qPCR) analysis. It is designed to perform real-time detection and quantification of nucleic acid sequences during the amplification process.
Automatically generated - may contain errors
Lab products found in correlation
Mirnaeasy mini kit, by Qiagen (5 mentions)
Power sybr green rt pcr reagents kit, by Quantabio (3 mentions)
Cdna synthesis kit, by OriGene (2 mentions)
Power sybr green rt pcr reagents kit, by Thermo Fisher Scientific (1 mentions)
Sybr green rt pcr kit, by Thermo Fisher Scientific (1 mentions)
Cdna synthesis kit, by Quantabio (1 mentions)
5 protocols using qpcr instrument
1
Quantitative Real-Time PCR Analysis
2
Quantitative Real-Time PCR Analysis of Target mRNAs
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To analyze the expression of target mRNAs, total RNA was isolated using the miRNAeasy Mini Kit (QIAGEN, Germantown, MD, USA) and reverse-transcribed to cDNAs using cDNA synthesis kit (OriGene, Rockville, MD, USA). The generated cDNA was amplified using power SYBR green RT-PCR reagents kit (Applied Biosystems, Foster City, CA, USA). The reaction was run at 95 °C for 10 min, which was followed by 40 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s, on a qPCR Instrument (Quantabio, Beverly, MA, USA). The relative transcript expression levels were measured by quantitative real-time PCR using the SYBR Green-based method. The average fold changes were calculated based on 18S in the threshold cycle (Cq). All the primer sequences are in Table 1 .
3
Quantitative Real-Time PCR for miRNA
4
Quantitative Analysis of Gene Expression
5
Quantification of miRNA Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was isolated using miRNAeasy Mini Kit (QIAGEN 217004) and reverse-transcribed to cDNA using cDNA synthesis kit (Quantabio 101414-106). The cDNA was amplified using power SYBR green RT-PCR reagents kit (Quantabio 101414-276). The reaction was run at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s, 60 °C for 30 s, and 72 °C for 30 s, on a qPCR Instrument (Quantabio). All RT-PCR was performed in quadruplicate and the average fold changes were calculated based on 18S in the threshold cycle (Cq). Primer sequences are in the Supplementary Table 1 .
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