The TLR7 agonist gardiquimod (Sigma) was dissolved in DMSO (Sigma) at a concentration of 100 μg/ml and stored in aliquots at −20°C. gardiquimod was added to cell cultures at a working concentration of 0.5 μg/ml. Antibodies for western blot include primary antibodies: rabbit anti-phospho-p65 (Ser536) (3033, Cell Signaling Technology), rabbit anti-p65 (4764, Cell Signaling Technology), rabbit anti-TLR7 (ab24184, Abcam), mouse anti-Lamp1 (H4A3, Santa Cruz Biotechnology), rabbit anti-IFN-α (YT5170, Immunoway), rabbit anti-GAPDH (AF1186, Beyotime) and secondary antibodies: HRP conjugated goat anti-rabbit IgG (H+L) (A0208, Beyotime), Alexa Fluor 594 goat anti-rabbit IgG (H+L) (A-11012, Thermo Fisher Scientific) and Alexa Fluor 488 goat anti-mouse IgG (H+L) (A-11017, Thermo Fisher Scientific). Antibodies for flow cytometry analysis were listed in Supplementary Table 1
Mouse anti lamp1 h4a3
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
Mouse anti-Lamp1 (H4A3) is a primary antibody that recognizes the lysosome-associated membrane protein 1 (Lamp1) in mouse samples. Lamp1 is a glycoprotein that is expressed on the lysosomal membrane and is commonly used as a marker for lysosomes.
Automatically generated - may contain errors
Lab products found in correlation
Gardiquimod, by Merck Group (1 mentions)
Rabbit anti phospho p65 ser536, by Cell Signaling Technology (1 mentions)
Alexa fluor 594 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Alexa fluor 488 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated goat anti rabbit igg h l, by Beyotime (1 mentions)
Rabbit anti parp 9542, by Cell Signaling Technology (1 mentions)
Rabbit anti lc3b l7543, by Merck Group (1 mentions)
Mouse anti lamp2 sc 18822, by Santa Cruz Biotechnology (1 mentions)
2 protocols using mouse anti lamp1 h4a3
1
TLR7 Agonist Gardiquimod Activation Protocol
2
Protein Detection and Autophagy Assays
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Rabbit anti-LC3B (L7543) and mouse anti-betaActin (A5441) were from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Guinea pig anti-p62 (GP62-C) was from PROGEN Biotechnik GmbH (Heidelberg, Germany). Rabbit anti-mTOR (2972S), rabbit anti-p-mTOR (2971S), rabbit anti-p-Akt (4058S), rabbit anti-Akt (9272S) and rabbit anti-PARP (9542) were from Cell Signaling (Danvers, MA, USA). Rabbit-anti-p-PERK (sc-32577), rabbit anti-GRP78 (H-129), mouse anti-Lamp1 (H4A3) and mouse-anti-Lamp2 (sc-18822) were from Santa Cruz Biotechnology (Heidelberg, Germany).
ML-9 (105637) was from Enzo Life Sciences (Villeurbanne, France). Acridine orange (A-3568) was from Life Technologies (Grand Island, NY, USA). Bafilomycin A1 (1334) was from Tocris (Bristol, UK). Chloroquine (C6628), Wortmannin (W1628) and trypan blue solution (T8154) were from Sigma-Aldrich.
ML-9 (105637) was from Enzo Life Sciences (Villeurbanne, France). Acridine orange (A-3568) was from Life Technologies (Grand Island, NY, USA). Bafilomycin A1 (1334) was from Tocris (Bristol, UK). Chloroquine (C6628), Wortmannin (W1628) and trypan blue solution (T8154) were from Sigma-Aldrich.
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