2-Cobaltocenium amidoethyl methacrylate hexafluorophosphate (CoAEMAPF6) was synthesized according to our earlier report.51 (link) 2-Aminoethyl methacrylate hydrochloride (90%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC-HCl, 98%), 4-(dimethylamino) pyridine and tetrabutylammonium chloride (TBACl) were purchased from Aldrich and used as received. Tris buffer solution was prepared from tris(hydroxymethyl)aminomethane (Tris base) (Sigma-Aldrich) and concentrated hydrochloric acid (Sigma-Aldrich). Poly(sodium styrene sulfonate) (Mn = 70,000 g/mol) was purchased from Sigma as an anionic polyelectrolyte. The fluorescent probe 5(6)-carboxyfluorescein (as a mixture of isomers) was purchased from Sigma-Aldrich. Displacer anion solutions were prepared from succinic acid (Sigma), tricarballylic acid and 1,4-cyclohexanedicarboxylic acid (Acros), 1,2,3,4-butanetetracarboxylic acid and pimelic acid (Alfa Aesar), trimesic acid and 1,3,5-cyclohexanetricarboxylic acid (TCI America). Water was from Thermo Scientific with ion conductivity at 18.20 MΩ. Azobisisobutyronitrile (AIBN) was recrystallized from methanol before use. All other chemicals were from commercial sources and used as received.
Tris hydroxymethyl aminomethane tris base
Manufactured by Merck Group
Sourced in United States
Tris(hydroxymethyl)aminomethane, also known as Tris base, is a chemical compound commonly used as a buffer in various laboratory applications. It is a white, crystalline solid that is highly soluble in water and has a slightly basic pH. Tris base is primarily used to maintain a stable pH in biological and biochemical experiments, facilitating the optimal functioning of enzymes and other biomolecules.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (2 mentions)
Succinic acid, by Merck Group (1 mentions)
Concentrated hydrochloric acid, by Merck Group (1 mentions)
2 aminoethyl methacrylate hydrochloride, by Merck Group (1 mentions)
Tetrabutylammonium chloride tbacl, by Merck Group (1 mentions)
Tricarballylic acid, by Merck Group (1 mentions)
Linoleic acid, by Merck Group (1 mentions)
Oleanolic acid, by Merck Group (1 mentions)
Hyaluronic acid, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by RCI Labscan (1 mentions)
Trolox, by Merck Group (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by RCI Labscan (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Merck Group (1 mentions)
Potassium persulphate, by Merck Group (1 mentions)
Ferrous sulfate, by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl s triazine, by Merck Group (1 mentions)
N hexane, by RCI Labscan (1 mentions)
Glacial acetic acid, by RCI Labscan (1 mentions)
7 protocols using tris hydroxymethyl aminomethane tris base
1
Synthesis of Cobaltocenium Methacrylate Monomer
2
Antioxidant, Anti-Enzymatic, and Anti-Tyrosinase Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
L - Ascorbic acid, EGCG, oleanolic acid, Trolox, DPPH, ABTS, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), potassium persulphate (K2S2O8), ferrous sulfate (FeSO4), linoleic acid, collagenase from Clostridium histolyticum (EC 3.4.24.3), elastase from porcine pancreas (EC 3.4.21.36), hyaluronidase from bovine testes (EC 3.2.1.35), lyophilized tyrosinase from mushroom (EC 1.14.18.1), synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), N-succinyl-Ala-Ala-Ala-p-nitroaniline (AAAPVN), hyaluronic acid, L -3,4-dihydroxyphenylalanine (L -DOPA), L -tyrosine, N-tris(hydroxymethyl)methylglycine (tricine), bovine serum albumin (BSA), sodium chloride (NaCl), calcium chloride (CaCl2), sodium acetate trihydrate (CH3COONa·3H2O), ammonium thiocyanate (NH4SCN), ferrous chloride (FeCl3), disodium phosphate (Na2HPO4), monosodium phosphate (NaH2PO4), SLS, and Tris(hydroxymethyl)aminomethane (Tris base) were purchased from Sigma-Aldrich (Schnelldorf, Germany). DMSO, n-hexane, 95% v/v ethanol, glacial acetic acid, and hydrochloric acid (HCl) were purchased from RCI Labscan Ltd. (Bangkok, Thailand).
+ Expand
3
Temozolomide and LCC-09 Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Temozolomide (TMZ; ≥98% HPLC, T2577) was purchased from Sigma-Aldrich, while LCC-09 was synthesized as previously described (16). Stock solution of TMZ or LCC-09 were dissolved in dimethyl sulfoxide (10 mg/mL) (DMSO; Sigma Aldrich Co., St. Louis, MO, USA) and stored at −20 °C away from light before use, then diluted in sterile culture medium just before use. Gibco® RPMI-1640, fetal bovine serum (FBS), trypsin/EDTA, phosphate buffered saline (PBS), sulforhodamine B (SRB) medium, tris(hydroxymethyl)aminomethane (TRIS) base and acetic acid were all purchased from Sigma Aldrich Co. (St. Louis, MO, USA), A BCA protein assay was purchased from Pierce (Rockford, IL, USA) and the polyvinylidene difluoride (PVDF) membrane was purchased from Millipore (Bedford, MA, USA).
4
Membrane Vesicle Transport Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Membrane vesicles from
HEK293 cells overexpressing the human BCRP, P-gp, MRP2, MRP3, and
MRP4 as well as the stably transfected hOATP2B1-MDCK-II cells were
provided by Solvo Biotechnology (Budapest, Hungary). Cell culture
medium, i.e., Dulbecco’s modified Eagle’s medium (DMEM),
fetal bovine serum, 1% GlutaMAX-1, 100 U/mL penicillin, and 100 μg/mL
streptomycin were obtained from Sigma-Aldrich (St. Louis, MO). Furosemide,
diclofenac, estrone-3-sulfate, rosuvastatin, adenosine 5′-triphosphate
(ATP) disodium salt, adenosine 5′-monophosphate (AMP) monohydrate,
glutathione, tris[hydroxymethyl]aminomethane (tris-base), MgCl2, NaCl, sucrose, and 3-[N-morpholino] propane
sulfonic acid (MOPS) were purchased from Sigma-Aldrich (St. Louis,
MO). Bovine serum albumin (BSA), membrane protein extraction kit,
and trypsin digestion reagents were obtained from Thermo Fisher Scientific
(Rockford, IL). Hanks’ balanced salt solution (HBSS), phosphate
buffer saline (PBS), acetonitrile, and formic acid were purchased
from Thermo Fisher Scientific (Rockford, IL). The synthetic unlabeled
and stable labeled peptides were purchased from New England Peptides
(Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively.
Multiscreen HTS Vacuum Manifold and 96-well filter plates with class
B glass fiber filters were purchased from EMD Millipore (Billerica,
MA).
HEK293 cells overexpressing the human BCRP, P-gp, MRP2, MRP3, and
MRP4 as well as the stably transfected hOATP2B1-MDCK-II cells were
provided by Solvo Biotechnology (Budapest, Hungary). Cell culture
medium, i.e., Dulbecco’s modified Eagle’s medium (DMEM),
fetal bovine serum, 1% GlutaMAX-1, 100 U/mL penicillin, and 100 μg/mL
streptomycin were obtained from Sigma-Aldrich (St. Louis, MO). Furosemide,
diclofenac, estrone-3-sulfate, rosuvastatin, adenosine 5′-triphosphate
(ATP) disodium salt, adenosine 5′-monophosphate (AMP) monohydrate,
glutathione, tris[hydroxymethyl]aminomethane (tris-base), MgCl2, NaCl, sucrose, and 3-[N-morpholino] propane
sulfonic acid (MOPS) were purchased from Sigma-Aldrich (St. Louis,
MO). Bovine serum albumin (BSA), membrane protein extraction kit,
and trypsin digestion reagents were obtained from Thermo Fisher Scientific
(Rockford, IL). Hanks’ balanced salt solution (HBSS), phosphate
buffer saline (PBS), acetonitrile, and formic acid were purchased
from Thermo Fisher Scientific (Rockford, IL). The synthetic unlabeled
and stable labeled peptides were purchased from New England Peptides
(Boston, MA) and Thermo Fisher Scientific (Rockford, IL), respectively.
Multiscreen HTS Vacuum Manifold and 96-well filter plates with class
B glass fiber filters were purchased from EMD Millipore (Billerica,
MA).
5
Antioxidant Evaluation of Pomegranate Seed Oil
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Pomegranate seed oil (PSO), dimethylsulfoxide (DMSO), 1-1-diphenyl-2-picrylhydrazyl (DPPH), absolute ethanol, formaldehyde, tris-(hydroxymethyl)-amino methane (Tris-base), ethylenediaminetetracetic acid disodium salt (Na2EDTA), triton X-100, and ethidium bromide (EtBr) were purchased from Sigma-Aldrich (USA). Kits for all biochemical parameters were purchased from Bio-diagnostic Company (Giza, Egypt).
6
Thiolated DNA Probe Sequence Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC purified 22-mer ssDNA sequences (thiolated probe sequence: HS-(CH2)6-5′-CTTCACGATTGCCACTTTCCAC-3′ (pDNA), designed to be fully complementary to the target sequence, and target sequence: 5′-GTGGAAAGTGGCAATCGTGAAG-3′ (tDNA)) were purchased from Biomers (Ulm, Germany) and used as received.
Tris[hydroxymethyl]amino-methane (Tris base), ethylenediaminetetraacetic acid (EDTA), and 6-Mercapto-1-hexanol (HS–(CH2)6–OH, MCH) were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium chloride (NaCl) was purchased from Merck (Darmstadt, Germany).
All DNA and MCH solutions were prepared using ultrapure water from Millipore (resistivity 18.2 MΩ·cm). The experiments were performed in TE buffer (10 mM Tris, 1 mM EDTA, 1 M NaCl, pH adjusted to 7.2 using HCl (Fluka, Buchs, Switzerland)). EDTA was used to chelate metal cations, such as Mg2+, which are involved in the activation of DNase [50 (link)]. The presence of EDTA blocks DNase activity, preventing DNA degradation.
Tris[hydroxymethyl]amino-methane (Tris base), ethylenediaminetetraacetic acid (EDTA), and 6-Mercapto-1-hexanol (HS–(CH2)6–OH, MCH) were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium chloride (NaCl) was purchased from Merck (Darmstadt, Germany).
All DNA and MCH solutions were prepared using ultrapure water from Millipore (resistivity 18.2 MΩ·cm). The experiments were performed in TE buffer (10 mM Tris, 1 mM EDTA, 1 M NaCl, pH adjusted to 7.2 using HCl (Fluka, Buchs, Switzerland)). EDTA was used to chelate metal cations, such as Mg2+, which are involved in the activation of DNase [50 (link)]. The presence of EDTA blocks DNase activity, preventing DNA degradation.
7
PLGA-based Membrane Probe Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Poly(D,L-lactide-co-glycolide) (PLGA, lactide 50 mol %, glycolide 50 mol %, acid terminated, Mn 24 000, PDI 1.7, and Mn 8800, PDI 1.7) was purchased from Sigma-Aldrich and used as received. Sodium phosphate monobasic (>99.0%, Sigma-Aldrich) and sodium phosphate dibasic dihydrate (>99.0%, Sigma-Aldrich) were used to prepare 20 mM phosphate buffer solutions at pH 7.4. Tris(hydroxymethyl)aminomethane (Tris base, >99.8%, Sigma-Aldrich) and HCl (1 M) were used to prepare 20 mM Tris buffer with a pH of 7.4. Milli-Q water (Millipore) was used in all experiments. Membrane probe dSQ12S was synthesized as described before. 78
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!