Human breast cancer cell lines MCF10A, MDA-MB-468 (MDA-468), BT20, and MDA-MB-231 (MDA-231) were purchased from ATCC (Manassas, VA, USA) and cultured as instructed. Bone-metastatic MDA-MB-231BoM (231-BoM), and brain-metastatic MDA-MB-231BrM (231-BrM) cell lines are from the Massagué laboratory and were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin [43 (link), 44 (link)]. Trastuzumab-resistant BT474 (BT474-TtzmR) were a kind gift from Dr. Dihua Yu [45 (link)]. All cell lines have been authenticated using the standard method, and tested for mycoplasma. If tested positive for mycoplasma contamination, cells were treated until free of contamination before use. Pacritinib, ruxolitinib, sonidegib, and vismodegib were obtained from AdooQ Bioscience (Irvine, CA, USA) and stock solutions were prepared using dimethyl sulfoxide (DMSO) for administration in cell culture.
Vismodegib
Manufactured by Adooq
Sourced in United States
Vismodegib is a small molecule inhibitor of the Hedgehog signaling pathway. It is used as a research tool in laboratory settings to study the role of Hedgehog signaling in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using vismodegib
1
Breast Cancer Cell Lines and Inhibitors
2
Breast Cancer Cell Lines and Inhibitors
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Human breast cancer cell lines MCF10A, MDA-MB-468 (MDA-468), BT20, and MDA-MB-231 (MDA-231) were purchased from ATCC (Manassas, VA, USA) and cultured as instructed. Bone-metastatic MDA-MB-231BoM (231-BoM), and brain-metastatic MDA-MB-231BrM (231-BrM) cell lines are from the Massagué laboratory and were cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin [43 (link), 44 (link)]. Trastuzumab-resistant BT474 (BT474-TtzmR) were a kind gift from Dr. Dihua Yu [45 (link)]. All cell lines have been authenticated using the standard method, and tested for mycoplasma. If tested positive for mycoplasma contamination, cells were treated until free of contamination before use. Pacritinib, ruxolitinib, sonidegib, and vismodegib were obtained from AdooQ Bioscience (Irvine, CA, USA) and stock solutions were prepared using dimethyl sulfoxide (DMSO) for administration in cell culture.
3
Benzidine Modulates Breast Cancer Stem Cells
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UM-UC-3 and EJ cells were cultured in RPMI-1640 supplemented with 10% fetal bovine serum (serum-supplemented medium; SSM) previously. Then cells were collected and resuspended (2,500 cells/ml) in serum-free medium (SFM; DMEM/F12) at a density of 5,000 cells/well (24-well plate), with 20 ng/ml EGF, 20 ng/ml bFGF and 2% B27. Photographic images of the tumorspheres were captured under a light microscope using a magnification of ×100 (Nikon Corporation). Benzidine was dissolved in DMSO and stored at −20°C. To explore the activating effect of benzidine on BCSCs, various concentrations of benzidine (0, 0.001, 0.005 and 0.025 µM) were added to the cells in each well, in the absence or presence of vismodegib (10 µM) (Adooq Bioscience). The final concentration of DMSO diluted in the culture medium was <1%. After 1 week of treatment at 37°C, the number of individual tumorspheres >100 µm in diameter was counted under a light microscope.
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