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Nod cg prkdcscid j nod scid

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NOD.Cg-Prkdcscid/J (NOD/SCID) is a mouse strain that is deficient in the Prkdc gene, resulting in severe combined immunodeficiency (SCID). This strain lacks functional T and B cells, making it a useful model for xenograft and cell transplantation studies.

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Lab products found in correlation

Cd1 mice 022, by Charles River Laboratories (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) B6 bks d leprdb j db db, by Jackson ImmunoResearch (1 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

NOD/SCID mice (NOD.Cg-Prkdcscid/J NOD.Cg-Prkdcscid/J NOD/SCID

2 protocols using nod cg prkdcscid j nod scid

1

Mouse Strain Characterization for Research

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C57BL/6J (JAX 000664), FVB/NJ (FVB, JAX 001800), 129S1/SvImJ (SV129, JAX 002448), B6.Cg-Gt(ROSA)26Sortm14(CAG-TT)Hze/J (49 (link)) (R26R-TT, JAX 007914), and NOD.Cg-Prkdcscid/J (NOD/SCID, JAX 001303) were purchased from the Jackson Laboratory. CD1 mice (022) were purchased from Charles River Laboratories. Flt3Cre+ ROSAmTmG/mTmG mice (50 (link)–52 (link)) were a gift from Slava Epelman, University of Toronto, Toronto, Ontario, Canada. Tie2CreERT2 mice (gift of Ye Zheng, Cincinnati Children’s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio, USA), previously described (16 (link)), were crossed with the commercially available R26R-TT mice (above) to generate (Tie2TT) mice. The primers used for genotyping the abovementioned Cre or ROSA mice are shown in Supplemental Table 3. To induce Cre expression in Tie2CreERT2 and Cdh5(PAC)-CreERT2 mice, 50 mg/kg TAM was injected into the animals i.p. at appropriate time points (3–5 days).
Lin Y., Banno K., Gil C.H., Myslinski J., Hato T., Shelley W.C., Gao H., Xuei X., Liu Y., Basile D.P., Yoshimoto M., Prasain N., Tarnawsky S.P., Adams R.H., Naruse K., Yoshida J., Murphy M.P., Horie K, & Yoder M.C. (2023). Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans. JCI Insight, 8(5), e164781.
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2

Metabolic Phenotyping of Murine Diabetes Models

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C57BL/6J wild-type (WT) mice (Stock No. 000664), B6.BKS(D)-Leprdb/J (db/db) (Stock No. 000697), B6.Cg-Tg(Ins1-EGFP)1Hara/J (MIP-GFP) (Stock No. 006864), and NOD.Cg-PrkdcScid/J (NOD-Scid) (Stock No. 001303) were obtained from the Jackson Laboratory. Male and female mice were group-housed under a standard rodent chow at ambient temperature (22°C), 48% humidity in average and a 12 hour light-dark cycle in individually ventilated cages. Food and water were provided ad libitum. Body composition was analyzed using EchoMRI. Samples were obtained from female mice at 10 weeks of age and male mice at 10, 18 or 40 weeks of age. WT and MIP-GFP transgenic mice were fed a regular chow diet (RD; 5053, LabDiet) or a 60% high fat diet (HFD; D12492i, Research Diets) for 6 and 12 weeks after weaning. C57BL/6J wild-type fed with RD and HFD for 6 weeks were fasted 6 hours for glucose tolerance tests (GTT) and given an i.p. injection of D-Glucose (1g/kg). For insulin tolerance tests (ITT), mice were fasted for 4 hours and given an i.p. injection of insulin (0.75 units/kg body weight). Blood samples were taken at different times from the tail vein and measured with a glucometer.
Mankin A.S., Zyrianova I.M., Kagramanova V.K, & Garrett R.A. (1992). Introducing mutations into the single-copy chromosomal 23S rRNA gene of the archaeon Halobacterium halobium by using an rRNA operon-based transformation system.. Proceedings of the National Academy of Sciences of the United States of America, 89(14), 6535-6539.
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