Gex4t 1 vector

Manufactured by Merck Group

The GEX4t-1 vector is a laboratory equipment product designed for genetic engineering applications. It serves as a tool for cloning and expressing target genes in various cell lines. The core function of the GEX4t-1 vector is to facilitate the insertion and controlled expression of genetic material in a research environment.

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Lab products found in correlation

Glutathione agarose, by Thermo Fisher Scientific (2 mentions)

2 protocols using gex4t 1 vector

Producing Polyclonal Antibodies for HyFat and HyDs

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Peptides corresponding to the C-terminal portions of HyFat (residues 4,191 to 4,393) and HyDs (residues 9,144 to 9,799) were expressed as GST fusions using the GEX4t-1 vector (Millipore) in Escherichia coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified proteins were used to immunize guinea pigs (Cocalico Biologicals) and rats (Covance). Polyclonal antibodies were purified by binding to antigen immobilized on glutathione-agarose beads and elution with 100 mM glycine (pH 2.5) into 1 M Tris, pH 8.0.

Brooun M., Klimovich A., Bashkurov M., Pearson B.J., Steele R.E, & McNeill H. (2020). Ancestral roles of atypical cadherins in planar cell polarity. Proceedings of the National Academy of Sciences of the United States of America, 117(32), 19310-19320.

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HyYap Protein Expression and Purification

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A peptide corresponding to residues 1 to 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in Escherichia coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified protein was used to immunize guinea pigs (Cocalico Biologicals).

Brooun M., Salvenmoser W., Dana C., Sudol M., Steele R., Hobmayer B, & McNeill H. (2022). The Hippo pathway regulates axis formation and morphogenesis in Hydra. Proceedings of the National Academy of Sciences of the United States of America, 119(29), e2203257119.

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