The tomato plants were grown in the greenhouse of the Mediterranean Agronomic Institute of Chania under regular conditions. The leaf abscission explants were prepared from stems of mature tomato plants comprising around 10 cm of stem section with the petiole after removing the leaf blade. The explants were then placed upright in beakers with water and treated with approximately 15 μl L−1 of ethylene in jars with an open system installation for 48 h. The flower AZs were fixed in 10% formalin, then dehydrated through a series of graded ethanol and embedded in paraffin by using a paraffin embedding station (Leica TP1020). The AZs were then used to prepare longitudinal sections with a rotary microtome (Leica RM2135) for image analysis with a stereomicroscope Leica MZ7.5 (Meyer Instruments Inc.), a camera ProgRes® C12 plus (Jenoptik), and the CapturePro® 2.1 Image Acquisition Software.
Progres c12plus
Manufactured by Jenoptik
Sourced in Germany
The ProgRes C12plus is a digital camera for microscopy applications. It features a high-resolution 12-megapixel CMOS sensor and supports various image acquisition modes. The camera is designed to provide high-quality images for scientific and research purposes.
Automatically generated - may contain errors
3 protocols using progres c12plus
1
Tomato Leaf Abscission and Flower Anatomy
2
Confocal Microscopy of Whole Mounts
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Whole mounts were scanned with a confocal laser scanning microscope (CLSM, TCS SP5, Leica Microsystems, Wetzlar, Germany) with a 10 × oil immersion objective lens. Most image stacks were generated with a scan velocity of 200 Hz and a format of 1024 × 1024. A line average of 2 was used. The Cy3 and background signal was detected with a DPSS (561 nm) laser while Cy5‐fluorescence was detected with a HeNe (633 nm) laser. The data stacks were processed with Amira 5.33 (Advanced 3D Visualization and Volume Modeling, RRID: nif‐0000‐00262). Images from the peroxidase‐labeled preparations were captured using a compound microscope (Zeiss Axioskop) equipped with a digital camera (ProgRes C12plus, Jenoptik, Jena, Germany). The size, contrast, and brightness of the images were adjusted using Photoshop CS5 (Adobe Systems, Ireland). The composition of the figures and the lettering was done using Illustrator CS5 (Adobe Systems, Ireland).
3
Imaging and Tracing of Cerebellar Neurons
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Images from selected sections were captured by a digital camera (ProgRes C12plus, Jenoptik) mounted on a transmission light microscope (Axioskop, Zeiss, Oberkochen, Germany). Images were optimized in contrast and brightness using Photoshop CS3 and CC 2017 (Adobe Systems, San Jose, CA). Some figures show superimposed images from two to three adjacent sections (see Figure legends). In Figures 8j and 10a images were obtained by montaging adjacent areas from the sections using the "darken" function of Adobe Photoshop.
Immunostained neurons of the CBL and CBU were traced from serial brain sections through a camera lucida attachment on a compound microscope (Leitz, Wetzlar, Germany). The drawings were digitized with a scanner (CanoScan 9000F Mark II, Canon, Tokyo, Japan).
Images and the 2D-reconstructions were assembled in CorelDraw software (Ottawa, Ontario, Canada). Positional information in all fig-
ures is given with regard to the animal's body axis.
Immunostained neurons of the CBL and CBU were traced from serial brain sections through a camera lucida attachment on a compound microscope (Leitz, Wetzlar, Germany). The drawings were digitized with a scanner (CanoScan 9000F Mark II, Canon, Tokyo, Japan).
Images and the 2D-reconstructions were assembled in CorelDraw software (Ottawa, Ontario, Canada). Positional information in all fig-
ures is given with regard to the animal's body axis.
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