Aqueous PEG polymer solutions (10 wt.%) were filtered through a poly(vinylidene fluoride) filter (0.22 μm, Whatman, Fisher Scientific, USA). A total of 20 droplets (0.5 μl/droplet) for each PEG solution were pipetted onto a slide glass and placed inside the cryostage (LTS120, Linkam Scientific Instruments Ltd, UK). The cryostage was rapidly cooled to 5 °C at a rate of 30 °C/min and kept it for 3 min. The sample was then cooled to –30 °C at a cooling rate of –1 °C/min. The frozen fraction of the droplets was monitored. The nucleation temperatures defined by the temperatures at which half of the droplets were frozen were recorded [19 (link)].
Lts120
Manufactured by Linkam
Sourced in United Kingdom
The LTS120 is a temperature-controlled sample stage designed for use in a variety of microscopy and analytical applications. It is capable of maintaining sample temperatures between -196°C and 120°C. The LTS120 provides precise temperature control and stability, allowing for the observation and analysis of samples under controlled environmental conditions.
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Lab products found in correlation
9 protocols using lts120
1
Cryogenic Nucleation of PEG Droplets
2
Photocrosslinking of Polymer Micelles and Emulsions
3
Spatial Distribution of Fluorescent PCE
4
Characterization of Protein Spatial Distributions
5
Visualizing ELP-RBD and ssRNA1 Coacervation
6
Ice Recrystallization Inhibition Assay
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To compare ice recrystallization inhibition (IRI) activity, each polymer solution (1.0 wt.%, 10 μl) in phosphate buffered saline (PBS) was dropped using a micropipette through a plastic tube (with a height of 1 m and a diameter of 20 cm) onto a slide glass pre-cooled on a dry ice-chilled aluminum plate [8 (link), 9 (link)]. Upon impact, the drop instantly formed a 10 mm-diameter wafer of ice crystals on the slide glass, which was then transferred to a cryo-stage (LTS120, Linkam Scientific Instruments Ltd., UK) to be annealed at − 6 °C for 30 min. Afterwards, the cryomicroscopy images of the wafer were obtained using a microscope (CX40IT, Soptop, China) equipped with a 10X lens (UIS-2, Olympus Ltd., Japan) and a digital camera through crossed polarizers. The sizes of the ten largest crystals from each wafer were measured using the ImageJ software. The mean largest grain size was expressed as a relative size (%) obtained from PBS solution without polymers (control).
7
Microwire Fabrication and Characterization
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Gold microwires (length 1-2 mm, thickness 250 nm and width 2-16 µm) were patterned on glass and thermally oxidized silicon substrates by a photolithography method, as reported earlier. 29, (link)31, (link)34 (link) The substrates (and the microwires) were then coated with a 120-nm-thick film of 1 by vacuum thermal evaporation. The SCO-coated chip was connected to a source-meter (Keithley 2611A) via an eight-track connector 31 (link) and mounted on a variable-temperature microscope stage (Linkam Scientific Instruments, LTS120) to control the base temperature (Tb)
of the entire circuit. The microwire electrical characterizations and operation were carried out by applying current and not voltage bias in order to guarantee a reproducible Joule heating.
Optical reflectivity images were recorded at λ = 310 nm (LED source) and λ = 452 nm (halogen lamp with a spectral range reduced by a band-pass filter) using a custom-made optical microscope (compatible with UV light) equipped with a ×5 magnification objective (numerical aperture, NA= 0.12) and a CCD camera (Andor Technology Clara, 1392 × 1040 pixels of 6.45 µm size).
of the entire circuit. The microwire electrical characterizations and operation were carried out by applying current and not voltage bias in order to guarantee a reproducible Joule heating.
Optical reflectivity images were recorded at λ = 310 nm (LED source) and λ = 452 nm (halogen lamp with a spectral range reduced by a band-pass filter) using a custom-made optical microscope (compatible with UV light) equipped with a ×5 magnification objective (numerical aperture, NA= 0.12) and a CCD camera (Andor Technology Clara, 1392 × 1040 pixels of 6.45 µm size).
8
Ice Crystal Analysis via Splat Assay
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The size of the ice crystals was measured using a splat assay [21 (link)]. An amount of 10 μL of cryopreservation media containing polymeric supplements was dropped from a height of 1.4 m through a 10 cm diameter tube onto a polished aluminum (Al) block cooled with liquid nitrogen. The ice wafer (~1 cm) formed on the Al block was transferred to a precooled glass slide and then moved to a freezing stage connected to a temperature controller (LTS120; Linkam, Epsom Downs, UK) that had been equilibrated at −20 °C. The stage temperature was increased to −8 °C at a rate of 1 °C/min, and the wafer was then left to anneal for 30 min at −8 °C. The temperature was maintained during ice crystal measurement. The ice crystals were visualized using a microscope (DM2700M, Leica, Wetzlar, German) with crossed polarizing filters.
9
Vesicle Size Distribution Analysis
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Size distribution of the DPPC/Surfactants vesicles was studied by optical microscopy using a Zeiss (Jena, Germany) light microscope equipped with a Linkam LTS120 hot stage, controlled by a PE94 unit. Images were acquired with a Canon PowerShot S90 Wide Zoom digital camera. Sizing was performed using ImageJ software of calibrated images [31 (link)].
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