Three groups of AF cells were seeded in 96-well culture plates and added with Hoechst 33258 dye solution (Beyotime, C0003) followed by removing the culture media at 12, 24, and 48 h, respectively. The procedures were done as previously described [21 (link)]. After washing with PBS twice, AF cells were added with 0.5 ml 4% formaldehyde solution and fixed at RT for 10 min. Then, AF cells were washed with PBS for 2-4 times and incubated with Hoechst 33258 solution for 20-60 min in the dark at RT. Cells were observed under a fluorescence microscope under UV light followed by washing with PBS and adding with antifade reagent.
Hoechst 33258 dye solution
Manufactured by Beyotime
Sourced in China
Hoechst 33258 dye solution is a fluorescent dye that binds to the minor groove of DNA. It is commonly used in laboratory applications for the detection and quantification of DNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using hoechst 33258 dye solution
1
Quantifying AF Cell Nuclei with Hoechst
2
Quantitative Analysis of PC Cell Nuclei
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PC cells (1 × 105 cells/mL) in the logarithmic phase were seeded into six-well plates (3 mL per well) with cover glasses. The cells were incubated (37°C, 5% CO2) for 24 h, and then the culture solution was discarded. Then, the cells were fixed with fixation solution for 10 min and stained with Hoechst 33258 dye solution (Beyotime Biotechnology Co., Ltd., Shanghai, China) for 5 min away from light at room temperature. The slides with cells attached were placed on the anti-fluorescence quencher droplets. A fluorescence microscope (BX60, Olympus Optical Co., Ltd, Tokyo, Japan) was used to observe the slides, and images were collected.
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