Mouse cystatin c elisa kit

Wild-type BALB/c and C57BL/6J were treated with a single high dose (20 mg/kg) of dexamethasone given intraperitoneally (IP) at 9a.m. Dexamethasone 21-phosphate disodium salt (Sigma) was dissolved in PBS and filter sterilized prior to injection. Tail vein samples were taken 24- and 48-h following IP dosing, and plasma levels of CyC were determined with Mouse Cystatin C ELISA Kit (ab119590), Abcam.

Experiments with the C26 model were performed using 8-weeks old wild-type BALB/c male mice. Mice were inoculated subcutaneously in their right flank with the syngeneic C26 colorectal cancer cell line (2x10⁶ viable cells in 100μL RPMI vehicle) that induces cachexia. Prior to inoculation, C26 cells were dissociated with trypsin, followed by resuspension in FBS-free RPMI and counting of the viable cell concentration (trypan blue). C26-tumor bearing mice were termed pre-cachectic from 18 days post-inoculation and were defined as cachectic when their weight loss exceeded 15% from peak body weight. Plasma levels of CyC were determined with Mouse Cystatin C ELISA Kit (ab119590), Abcam. Corticosterone levels were quantified using Corticosterone ELISA (RE52211) from IBL International (TECAN).